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HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

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Related in: MedlinePlus

MCF-7 cells transfected with HOXB7-siRNA-NC-CY3 (fluorescent negative control) demonstrated red fluorescence (magnification, ×100). (A) Blank controls. Cells were incubated for 4–6 h with (B) 0.1 μM siRNA, (C) 0.2 μM siRNA or (D) 0.4 μM siRNA. HOXB7, homeobox B7.
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f3-mmr-12-04-4901: MCF-7 cells transfected with HOXB7-siRNA-NC-CY3 (fluorescent negative control) demonstrated red fluorescence (magnification, ×100). (A) Blank controls. Cells were incubated for 4–6 h with (B) 0.1 μM siRNA, (C) 0.2 μM siRNA or (D) 0.4 μM siRNA. HOXB7, homeobox B7.

Mentions: To optimize conditions of transfection, three different concentrations of HOXB7-siRNA-NC-CY3 (fluorescent negative control) were transfected into MCF-7 breast cancer cells. As shown in Fig. 3, all MCF-7 cells transfected with HOXB7-siRNA for 4–6 h exhibited red fluorescence under a fluorescence microscope. It was observed that the untransfected MCF-7 exhibited no red fluorescence (Fig. 3A) and the MCF-7 cells transfected with 0.1 μM siRNA demonstrated a little red fluorescence (Fig. 3B). By contrast, ~80% infected cells exhibited red fluorescence at an siRNA concentration of 0.2 μM as shown in Fig. 3C, while 90% exhibited red fluorescence at an siRNA concentration of 0.4 μM as shown in Fig. 3D, suggesting successful transfection. Thus, the concentration of 0.4 μM siRNA that exhibited the highest transfection efficiency was selected for the following experiments.


HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

MCF-7 cells transfected with HOXB7-siRNA-NC-CY3 (fluorescent negative control) demonstrated red fluorescence (magnification, ×100). (A) Blank controls. Cells were incubated for 4–6 h with (B) 0.1 μM siRNA, (C) 0.2 μM siRNA or (D) 0.4 μM siRNA. HOXB7, homeobox B7.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581815&req=5

f3-mmr-12-04-4901: MCF-7 cells transfected with HOXB7-siRNA-NC-CY3 (fluorescent negative control) demonstrated red fluorescence (magnification, ×100). (A) Blank controls. Cells were incubated for 4–6 h with (B) 0.1 μM siRNA, (C) 0.2 μM siRNA or (D) 0.4 μM siRNA. HOXB7, homeobox B7.
Mentions: To optimize conditions of transfection, three different concentrations of HOXB7-siRNA-NC-CY3 (fluorescent negative control) were transfected into MCF-7 breast cancer cells. As shown in Fig. 3, all MCF-7 cells transfected with HOXB7-siRNA for 4–6 h exhibited red fluorescence under a fluorescence microscope. It was observed that the untransfected MCF-7 exhibited no red fluorescence (Fig. 3A) and the MCF-7 cells transfected with 0.1 μM siRNA demonstrated a little red fluorescence (Fig. 3B). By contrast, ~80% infected cells exhibited red fluorescence at an siRNA concentration of 0.2 μM as shown in Fig. 3C, while 90% exhibited red fluorescence at an siRNA concentration of 0.4 μM as shown in Fig. 3D, suggesting successful transfection. Thus, the concentration of 0.4 μM siRNA that exhibited the highest transfection efficiency was selected for the following experiments.

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

Show MeSH
Related in: MedlinePlus