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HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

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Related in: MedlinePlus

Protein expression level of HOXB7 in two breast cancer cell lines measured by western blotting. The optical density for target protein is shown as the proportion of GAPDH optical density. (A) HOXB7 protein is overexpressed in MDA-MB-231 and MCF-7 breast cancer cell lines and (B) the expression levels in MCF-7 breast cancer cells were significantly higher compared with that in MDA-MB-231 breast cancer cells (***P<0.001). HOXB7, homeobox B7.
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f2-mmr-12-04-4901: Protein expression level of HOXB7 in two breast cancer cell lines measured by western blotting. The optical density for target protein is shown as the proportion of GAPDH optical density. (A) HOXB7 protein is overexpressed in MDA-MB-231 and MCF-7 breast cancer cell lines and (B) the expression levels in MCF-7 breast cancer cells were significantly higher compared with that in MDA-MB-231 breast cancer cells (***P<0.001). HOXB7, homeobox B7.

Mentions: To detect whether the protein expression level of HOXB7 was in accordance with the results of the mRNA expression level in breast cancer cells, western blotting was used in the same two human breast cancer cell lines, namely MDA-MB-231 and MCF-7 cells. The results of the western blotting revealed that HOXB7 protein was also overexpressed in MDA-MB-231 and MCF-7 breast cancer cell lines and the expression level in MCF-7 breast cancer cells was significantly higher compared with that in MDA-231 breast cancer cells (***P<0.001; Fig. 2). Therefore, combined with the result in Fig. 1, the MCF-7 human breast cancer cell line was selected for further experiments.


HOXB7-S3 inhibits the proliferation and invasion of MCF-7 human breast cancer cells.

Ma R, Zhang D, Hu PC, Li Q, Lin CY - Mol Med Rep (2015)

Protein expression level of HOXB7 in two breast cancer cell lines measured by western blotting. The optical density for target protein is shown as the proportion of GAPDH optical density. (A) HOXB7 protein is overexpressed in MDA-MB-231 and MCF-7 breast cancer cell lines and (B) the expression levels in MCF-7 breast cancer cells were significantly higher compared with that in MDA-MB-231 breast cancer cells (***P<0.001). HOXB7, homeobox B7.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581815&req=5

f2-mmr-12-04-4901: Protein expression level of HOXB7 in two breast cancer cell lines measured by western blotting. The optical density for target protein is shown as the proportion of GAPDH optical density. (A) HOXB7 protein is overexpressed in MDA-MB-231 and MCF-7 breast cancer cell lines and (B) the expression levels in MCF-7 breast cancer cells were significantly higher compared with that in MDA-MB-231 breast cancer cells (***P<0.001). HOXB7, homeobox B7.
Mentions: To detect whether the protein expression level of HOXB7 was in accordance with the results of the mRNA expression level in breast cancer cells, western blotting was used in the same two human breast cancer cell lines, namely MDA-MB-231 and MCF-7 cells. The results of the western blotting revealed that HOXB7 protein was also overexpressed in MDA-MB-231 and MCF-7 breast cancer cell lines and the expression level in MCF-7 breast cancer cells was significantly higher compared with that in MDA-231 breast cancer cells (***P<0.001; Fig. 2). Therefore, combined with the result in Fig. 1, the MCF-7 human breast cancer cell line was selected for further experiments.

Bottom Line: Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively.Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells.In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Key Laboratory of Tumor Biological Behavior of Hubei, Wuhan, Hubei 430071, P.R. China.

ABSTRACT
Homeobox B7 (HOXB7) has been found to be overexpressed in numerous types of human cancer. However, the role of HOXB7 in breast cancer remains to be elucidated. The aim of the present study was to investigate the effects of HOXB7 on the proliferation and invasion of breast cancer cells. Initially, reverse transcription quantitative polymerase chain reaction and western blotting were respectively employed to detect the mRNA and protein expression levels of the HOXB7 gene in the MDA‑MB‑231 and MCF‑7 human breast cancer cell lines. Subsequently, small interfering RNAs designed to interfere with the expression of HOXB7 were used to knockdown the expression of HOXB7 in the MCF‑7 cell line, the effects of which on cell proliferation, the apoptotic rate and invasion capacity were measured using a Cell Counting kit‑8 assay, flow cytometry and transwell chambers, respectively. The results demonstrated that HOXB7 mRNA and protein were all overexpressed in MDA‑MB‑231 and MCF‑7 breast cancer cell lines. Furthermore, HOXB7‑S3 effectively inhibited the proliferation and invasion of MCF‑7 breast cancer cells. In conclusion, these results demonstrated that HOXB7 may be a potential therapeutic target in human breast cancer.

Show MeSH
Related in: MedlinePlus