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Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema.

Wang Y, Bu J, Zhang Q, Chen K, Zhang J, Bao X - Mol Med Rep (2015)

Bottom Line: AQP1 was strongly expressed in the proximal tubules.The proportion of the AQP1‑positive area in kidney tissues from patients with NS with edema was significantly reduced, in comparison with the other two groups.The present study demonstrated the abnormal expression pattern of AQP1‑AQP4 in the kidney tissues of patients with NS, providing a basis for an improved understanding of the role of AQP in the pathogenesis of NS.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, P.R. China.

ABSTRACT
The association between the expression of aquaporins (AQPs) in kidney tissues and the occurrence of edema in nephrotic syndrome (NS) remains unclear. The current study aimed to investigate this association. A total of 54 patients with primary glomerular disease, diagnosed by renal biopsy, were divided into three groups: Control, NS without edema and NS with edema. The expression of AQP1, AQP2, AQP3 and AQP4 in kidney tissues from these patients was assessed using immunohistochemistry, and urinary AQP concentrations were quantified by ELISA. Comparison of the three groups was conducted using one way analysis of variance, independent samples t‑test or the Chi‑square test. AQP1 was strongly expressed in the proximal tubules. The proportion of the AQP1‑positive area in kidney tissues from patients with NS with edema was significantly reduced, in comparison with the other two groups. By contrast, the proportion of the AQP2‑positive area in the NS with edema group was significantly higher than that of the other two groups; significant differences were also observed between the control and NS without edema groups for this parameter. Urinary AQP2 concentrations in patients with NS (with and without edema) were significantly higher than that of the control group, and exhibited a significant positive correlation with kidney tissue AQP2 concentrations. The present study demonstrated the abnormal expression pattern of AQP1‑AQP4 in the kidney tissues of patients with NS, providing a basis for an improved understanding of the role of AQP in the pathogenesis of NS.

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Expression of AQP2 in kidney tissues in the control, NS without edema and NS with edema groups (magnification, ×400). Representative micrographs in each group demonstrate the immunostaining results for AQP2 in renal tubules (bottom panel) and glomeruli (top panel). AQP, aquaporin; NS, nephrotic syndrome.
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f2-mmr-12-04-5625: Expression of AQP2 in kidney tissues in the control, NS without edema and NS with edema groups (magnification, ×400). Representative micrographs in each group demonstrate the immunostaining results for AQP2 in renal tubules (bottom panel) and glomeruli (top panel). AQP, aquaporin; NS, nephrotic syndrome.

Mentions: AQP2 was strongly expressed in the collecting ducts, and was not detected in the glomerulus (Fig. 2). The AQP2 positive indices in patients with NS (with or without edema) were significantly higher than the index of the control group (both P<0.05; Table II). The quantity of AQP2 in the urine of patients with NS (with or without edema) was significantly higher than that in the control group (both P<0.05). However, no statistically significant difference was detected between the two NS groups (P>0.05, Table III). Notably, the quantity of AQP2 in the urine was not statistically associated with the expression of this protein in kidney tissues in all patients; while, a correlation was observed between these parameters in patients with NS (Table IV).


Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema.

Wang Y, Bu J, Zhang Q, Chen K, Zhang J, Bao X - Mol Med Rep (2015)

Expression of AQP2 in kidney tissues in the control, NS without edema and NS with edema groups (magnification, ×400). Representative micrographs in each group demonstrate the immunostaining results for AQP2 in renal tubules (bottom panel) and glomeruli (top panel). AQP, aquaporin; NS, nephrotic syndrome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581814&req=5

f2-mmr-12-04-5625: Expression of AQP2 in kidney tissues in the control, NS without edema and NS with edema groups (magnification, ×400). Representative micrographs in each group demonstrate the immunostaining results for AQP2 in renal tubules (bottom panel) and glomeruli (top panel). AQP, aquaporin; NS, nephrotic syndrome.
Mentions: AQP2 was strongly expressed in the collecting ducts, and was not detected in the glomerulus (Fig. 2). The AQP2 positive indices in patients with NS (with or without edema) were significantly higher than the index of the control group (both P<0.05; Table II). The quantity of AQP2 in the urine of patients with NS (with or without edema) was significantly higher than that in the control group (both P<0.05). However, no statistically significant difference was detected between the two NS groups (P>0.05, Table III). Notably, the quantity of AQP2 in the urine was not statistically associated with the expression of this protein in kidney tissues in all patients; while, a correlation was observed between these parameters in patients with NS (Table IV).

Bottom Line: AQP1 was strongly expressed in the proximal tubules.The proportion of the AQP1‑positive area in kidney tissues from patients with NS with edema was significantly reduced, in comparison with the other two groups.The present study demonstrated the abnormal expression pattern of AQP1‑AQP4 in the kidney tissues of patients with NS, providing a basis for an improved understanding of the role of AQP in the pathogenesis of NS.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, P.R. China.

ABSTRACT
The association between the expression of aquaporins (AQPs) in kidney tissues and the occurrence of edema in nephrotic syndrome (NS) remains unclear. The current study aimed to investigate this association. A total of 54 patients with primary glomerular disease, diagnosed by renal biopsy, were divided into three groups: Control, NS without edema and NS with edema. The expression of AQP1, AQP2, AQP3 and AQP4 in kidney tissues from these patients was assessed using immunohistochemistry, and urinary AQP concentrations were quantified by ELISA. Comparison of the three groups was conducted using one way analysis of variance, independent samples t‑test or the Chi‑square test. AQP1 was strongly expressed in the proximal tubules. The proportion of the AQP1‑positive area in kidney tissues from patients with NS with edema was significantly reduced, in comparison with the other two groups. By contrast, the proportion of the AQP2‑positive area in the NS with edema group was significantly higher than that of the other two groups; significant differences were also observed between the control and NS without edema groups for this parameter. Urinary AQP2 concentrations in patients with NS (with and without edema) were significantly higher than that of the control group, and exhibited a significant positive correlation with kidney tissue AQP2 concentrations. The present study demonstrated the abnormal expression pattern of AQP1‑AQP4 in the kidney tissues of patients with NS, providing a basis for an improved understanding of the role of AQP in the pathogenesis of NS.

Show MeSH
Related in: MedlinePlus