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MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression.

Lin Q, Mao Y, Song Y, Huang D - Mol Med Rep (2015)

Bottom Line: The effect of miR‑34a in PC12 cells has not yet been reported.It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression.These data indicate that miR‑34a may be important in neuropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.

ABSTRACT
MicroRNA‑34a (miR‑34a) is a direct target of p53 and was reported to induce cell cycle arrest, apoptosis and senescence. Inhibition of the NAD‑dependent deacetylase sirtuin‑1 (SIRT1) by miR‑34a leads to an increase in acetylated p53, which promotes cell apoptosis. B‑cell lymphoma 2 (Bcl‑2) is also involved in apoptosis, and was originally characterized with respect to its role in controlling outer mitochondrial membrane integrity. The effect of miR‑34a in PC12 cells has not yet been reported. In the present study, it was hypothesized that Bcl‑2 and SIRT1 may be critical downstream targets of miR‑34a that participate in apoptosis induction. miR‑34a mimics and inhibitors were transfected into PC12 cells, and the apoptosis and proliferation rates were compared between groups. It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression. These data indicate that miR‑34a may be important in neuropathy.

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Protein expression levels of SIRT1, Bcl-2 and ac-p53. The protein levels were determined by western blot analysis, using GAPDH levels as a loading control. All western blot data are presented as the mean ± standard deviation (n=3), *P<0.05 and **P<0.01. SIRT1, sirtuin 1; Bcl-2, B-cell lymphoma 2; miR, microRNA.
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f4-mmr-12-04-5709: Protein expression levels of SIRT1, Bcl-2 and ac-p53. The protein levels were determined by western blot analysis, using GAPDH levels as a loading control. All western blot data are presented as the mean ± standard deviation (n=3), *P<0.05 and **P<0.01. SIRT1, sirtuin 1; Bcl-2, B-cell lymphoma 2; miR, microRNA.

Mentions: To determine whether miR-34a expression levels correlate with the ac-p53, Bcl-2 and SIRT1 levels in PC12 cells, the expression of each protein was quantified by western blot analysis after transfection with miR-34a mimics or inhibitors. As Fig. 4 shows, compared with the control group, the expression levels of Bcl-2 (P<0.01) and SIRT1 (P<0.05) in the miR-34a mimic group were significantly reduced, while the levels of ac-p53 (P<0.05) were elevated in this group. In addition, the ac-p53 in miR-34a inhibitor group was reduced compared with the control group (P<0.05). The levels of SIRT1 in the miR-34a inhibitor group were significantly elevated compared with the miR-34a mimics group (P<0.05), however, the SIRT1 levels were not significantly different from the control group. The levels of Bcl-2 in the miR-34a inhibitor group were marginally elevated compared with the miR-34a mimic and the control groups, however the difference was not significant.


MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression.

Lin Q, Mao Y, Song Y, Huang D - Mol Med Rep (2015)

Protein expression levels of SIRT1, Bcl-2 and ac-p53. The protein levels were determined by western blot analysis, using GAPDH levels as a loading control. All western blot data are presented as the mean ± standard deviation (n=3), *P<0.05 and **P<0.01. SIRT1, sirtuin 1; Bcl-2, B-cell lymphoma 2; miR, microRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581806&req=5

f4-mmr-12-04-5709: Protein expression levels of SIRT1, Bcl-2 and ac-p53. The protein levels were determined by western blot analysis, using GAPDH levels as a loading control. All western blot data are presented as the mean ± standard deviation (n=3), *P<0.05 and **P<0.01. SIRT1, sirtuin 1; Bcl-2, B-cell lymphoma 2; miR, microRNA.
Mentions: To determine whether miR-34a expression levels correlate with the ac-p53, Bcl-2 and SIRT1 levels in PC12 cells, the expression of each protein was quantified by western blot analysis after transfection with miR-34a mimics or inhibitors. As Fig. 4 shows, compared with the control group, the expression levels of Bcl-2 (P<0.01) and SIRT1 (P<0.05) in the miR-34a mimic group were significantly reduced, while the levels of ac-p53 (P<0.05) were elevated in this group. In addition, the ac-p53 in miR-34a inhibitor group was reduced compared with the control group (P<0.05). The levels of SIRT1 in the miR-34a inhibitor group were significantly elevated compared with the miR-34a mimics group (P<0.05), however, the SIRT1 levels were not significantly different from the control group. The levels of Bcl-2 in the miR-34a inhibitor group were marginally elevated compared with the miR-34a mimic and the control groups, however the difference was not significant.

Bottom Line: The effect of miR‑34a in PC12 cells has not yet been reported.It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression.These data indicate that miR‑34a may be important in neuropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.

ABSTRACT
MicroRNA‑34a (miR‑34a) is a direct target of p53 and was reported to induce cell cycle arrest, apoptosis and senescence. Inhibition of the NAD‑dependent deacetylase sirtuin‑1 (SIRT1) by miR‑34a leads to an increase in acetylated p53, which promotes cell apoptosis. B‑cell lymphoma 2 (Bcl‑2) is also involved in apoptosis, and was originally characterized with respect to its role in controlling outer mitochondrial membrane integrity. The effect of miR‑34a in PC12 cells has not yet been reported. In the present study, it was hypothesized that Bcl‑2 and SIRT1 may be critical downstream targets of miR‑34a that participate in apoptosis induction. miR‑34a mimics and inhibitors were transfected into PC12 cells, and the apoptosis and proliferation rates were compared between groups. It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression. These data indicate that miR‑34a may be important in neuropathy.

Show MeSH
Related in: MedlinePlus