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MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression.

Lin Q, Mao Y, Song Y, Huang D - Mol Med Rep (2015)

Bottom Line: The effect of miR‑34a in PC12 cells has not yet been reported.It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression.These data indicate that miR‑34a may be important in neuropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.

ABSTRACT
MicroRNA‑34a (miR‑34a) is a direct target of p53 and was reported to induce cell cycle arrest, apoptosis and senescence. Inhibition of the NAD‑dependent deacetylase sirtuin‑1 (SIRT1) by miR‑34a leads to an increase in acetylated p53, which promotes cell apoptosis. B‑cell lymphoma 2 (Bcl‑2) is also involved in apoptosis, and was originally characterized with respect to its role in controlling outer mitochondrial membrane integrity. The effect of miR‑34a in PC12 cells has not yet been reported. In the present study, it was hypothesized that Bcl‑2 and SIRT1 may be critical downstream targets of miR‑34a that participate in apoptosis induction. miR‑34a mimics and inhibitors were transfected into PC12 cells, and the apoptosis and proliferation rates were compared between groups. It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression. These data indicate that miR‑34a may be important in neuropathy.

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Related in: MedlinePlus

Morphology of PC12 cells (A) after transfection with an miR-34a inhibitor (B) in the negative control group or (C) following transfection with miR-34a mimic. (A–C) The arrows indicate the non-adherent cells, which may be the apoptotic or senescent cells (magnifications, ×40). Representative images of SA β-gal staining of PC12 cells in (D) the miR-34a inhibitor group, (E) negative control group and (F) the miR-34a mimic group. Blue staining indicates the SA-β-gal-positive cells.
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f3-mmr-12-04-5709: Morphology of PC12 cells (A) after transfection with an miR-34a inhibitor (B) in the negative control group or (C) following transfection with miR-34a mimic. (A–C) The arrows indicate the non-adherent cells, which may be the apoptotic or senescent cells (magnifications, ×40). Representative images of SA β-gal staining of PC12 cells in (D) the miR-34a inhibitor group, (E) negative control group and (F) the miR-34a mimic group. Blue staining indicates the SA-β-gal-positive cells.

Mentions: The influence of miR-34a on cell senescence was then evaluated using the β-galactosidase staining assay. The number of non-adherent cells in the miR-34a mimic group was increasing compared with the miR-34a inhibitor group and the control group, however, no significant difference was identified between the miR-34a inhibitor group and the control group (Fig. 3A–C). SA-β-gal staining analysis showed that the miR-34a mimics greatly increased SA-β-gal activity (Fig. 3D–F). These results demonstrate that miR-34a increases PC12 cell apoptosis and senescence.


MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression.

Lin Q, Mao Y, Song Y, Huang D - Mol Med Rep (2015)

Morphology of PC12 cells (A) after transfection with an miR-34a inhibitor (B) in the negative control group or (C) following transfection with miR-34a mimic. (A–C) The arrows indicate the non-adherent cells, which may be the apoptotic or senescent cells (magnifications, ×40). Representative images of SA β-gal staining of PC12 cells in (D) the miR-34a inhibitor group, (E) negative control group and (F) the miR-34a mimic group. Blue staining indicates the SA-β-gal-positive cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581806&req=5

f3-mmr-12-04-5709: Morphology of PC12 cells (A) after transfection with an miR-34a inhibitor (B) in the negative control group or (C) following transfection with miR-34a mimic. (A–C) The arrows indicate the non-adherent cells, which may be the apoptotic or senescent cells (magnifications, ×40). Representative images of SA β-gal staining of PC12 cells in (D) the miR-34a inhibitor group, (E) negative control group and (F) the miR-34a mimic group. Blue staining indicates the SA-β-gal-positive cells.
Mentions: The influence of miR-34a on cell senescence was then evaluated using the β-galactosidase staining assay. The number of non-adherent cells in the miR-34a mimic group was increasing compared with the miR-34a inhibitor group and the control group, however, no significant difference was identified between the miR-34a inhibitor group and the control group (Fig. 3A–C). SA-β-gal staining analysis showed that the miR-34a mimics greatly increased SA-β-gal activity (Fig. 3D–F). These results demonstrate that miR-34a increases PC12 cell apoptosis and senescence.

Bottom Line: The effect of miR‑34a in PC12 cells has not yet been reported.It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression.These data indicate that miR‑34a may be important in neuropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.

ABSTRACT
MicroRNA‑34a (miR‑34a) is a direct target of p53 and was reported to induce cell cycle arrest, apoptosis and senescence. Inhibition of the NAD‑dependent deacetylase sirtuin‑1 (SIRT1) by miR‑34a leads to an increase in acetylated p53, which promotes cell apoptosis. B‑cell lymphoma 2 (Bcl‑2) is also involved in apoptosis, and was originally characterized with respect to its role in controlling outer mitochondrial membrane integrity. The effect of miR‑34a in PC12 cells has not yet been reported. In the present study, it was hypothesized that Bcl‑2 and SIRT1 may be critical downstream targets of miR‑34a that participate in apoptosis induction. miR‑34a mimics and inhibitors were transfected into PC12 cells, and the apoptosis and proliferation rates were compared between groups. It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression. These data indicate that miR‑34a may be important in neuropathy.

Show MeSH
Related in: MedlinePlus