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MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression.

Lin Q, Mao Y, Song Y, Huang D - Mol Med Rep (2015)

Bottom Line: The effect of miR‑34a in PC12 cells has not yet been reported.It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression.These data indicate that miR‑34a may be important in neuropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.

ABSTRACT
MicroRNA‑34a (miR‑34a) is a direct target of p53 and was reported to induce cell cycle arrest, apoptosis and senescence. Inhibition of the NAD‑dependent deacetylase sirtuin‑1 (SIRT1) by miR‑34a leads to an increase in acetylated p53, which promotes cell apoptosis. B‑cell lymphoma 2 (Bcl‑2) is also involved in apoptosis, and was originally characterized with respect to its role in controlling outer mitochondrial membrane integrity. The effect of miR‑34a in PC12 cells has not yet been reported. In the present study, it was hypothesized that Bcl‑2 and SIRT1 may be critical downstream targets of miR‑34a that participate in apoptosis induction. miR‑34a mimics and inhibitors were transfected into PC12 cells, and the apoptosis and proliferation rates were compared between groups. It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression. These data indicate that miR‑34a may be important in neuropathy.

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Related in: MedlinePlus

Effects of miR-34a mimics and inhibitors on cell proliferation determined by 3-(4,5-dimethylthiazol-2-yl)-2-5 diphenyltetrazolium bromide assays. Data are expressed as the mean ± standard deviation, **P<0.01, compared with the negative control group and ##P<0.01 compared with the miR-34a inhibitor group, n=5 for each group. miR, microRNA; OD, optical density.
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f1-mmr-12-04-5709: Effects of miR-34a mimics and inhibitors on cell proliferation determined by 3-(4,5-dimethylthiazol-2-yl)-2-5 diphenyltetrazolium bromide assays. Data are expressed as the mean ± standard deviation, **P<0.01, compared with the negative control group and ##P<0.01 compared with the miR-34a inhibitor group, n=5 for each group. miR, microRNA; OD, optical density.

Mentions: To assess the biological role of miR-34a during the proliferation of PC12 cells, cells were transiently transfected with miR-34a mimics or inhibitors, and the proliferation was measured 24, 48 and 72 h following transfection using an MTT assay. As shown in Fig. 1, following transfection with miR-34a mimics, the proliferation of PC12 cells was significantly decreased compared with that of the negative control group (P<0.01). The proliferation of the miR-34a inhibitor group was marginally higher than that of the negative control group; however, no significant difference was identified (P>0.05).


MicroRNA‑34a induces apoptosis in PC12 cells by reducing B‑cell lymphoma 2 and sirtuin‑1 expression.

Lin Q, Mao Y, Song Y, Huang D - Mol Med Rep (2015)

Effects of miR-34a mimics and inhibitors on cell proliferation determined by 3-(4,5-dimethylthiazol-2-yl)-2-5 diphenyltetrazolium bromide assays. Data are expressed as the mean ± standard deviation, **P<0.01, compared with the negative control group and ##P<0.01 compared with the miR-34a inhibitor group, n=5 for each group. miR, microRNA; OD, optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581806&req=5

f1-mmr-12-04-5709: Effects of miR-34a mimics and inhibitors on cell proliferation determined by 3-(4,5-dimethylthiazol-2-yl)-2-5 diphenyltetrazolium bromide assays. Data are expressed as the mean ± standard deviation, **P<0.01, compared with the negative control group and ##P<0.01 compared with the miR-34a inhibitor group, n=5 for each group. miR, microRNA; OD, optical density.
Mentions: To assess the biological role of miR-34a during the proliferation of PC12 cells, cells were transiently transfected with miR-34a mimics or inhibitors, and the proliferation was measured 24, 48 and 72 h following transfection using an MTT assay. As shown in Fig. 1, following transfection with miR-34a mimics, the proliferation of PC12 cells was significantly decreased compared with that of the negative control group (P<0.01). The proliferation of the miR-34a inhibitor group was marginally higher than that of the negative control group; however, no significant difference was identified (P>0.05).

Bottom Line: The effect of miR‑34a in PC12 cells has not yet been reported.It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression.These data indicate that miR‑34a may be important in neuropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China.

ABSTRACT
MicroRNA‑34a (miR‑34a) is a direct target of p53 and was reported to induce cell cycle arrest, apoptosis and senescence. Inhibition of the NAD‑dependent deacetylase sirtuin‑1 (SIRT1) by miR‑34a leads to an increase in acetylated p53, which promotes cell apoptosis. B‑cell lymphoma 2 (Bcl‑2) is also involved in apoptosis, and was originally characterized with respect to its role in controlling outer mitochondrial membrane integrity. The effect of miR‑34a in PC12 cells has not yet been reported. In the present study, it was hypothesized that Bcl‑2 and SIRT1 may be critical downstream targets of miR‑34a that participate in apoptosis induction. miR‑34a mimics and inhibitors were transfected into PC12 cells, and the apoptosis and proliferation rates were compared between groups. It was demonstrated that induction of miR‑34a promotes apoptosis and senescence, inhibits proliferation, and leads to marked alterations in SIRT1, Bcl‑12 and acetyl (ac)‑p53 expression. These data indicate that miR‑34a may be important in neuropathy.

Show MeSH
Related in: MedlinePlus