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MicroRNA‑20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing early growth response 2 expression.

Zhuo W, Ge W, Meng G, Jia S, Zhou X, Liu J - Mol Med Rep (2015)

Bottom Line: It was determined that miR‑20a expression is markedly upregulated in OS tissues and cells compared with the matched adjacent normal tissues and h‑FOB human osteoblast cell lines.Data from luciferase reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and translational levels.In conclusion, the data provide compelling evidence that miR‑20a functions as an onco‑miRNA, which is important in promoting cell proliferation in OS, and its oncogenic effect is mediated primarily through direct suppression of EGR2 expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Institute of Orthopedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710000, P.R. China.

ABSTRACT
MicroRNAs (miRNAs) are crucial in cancer development. However, the underlying mechanisms of miRNAs in osteosarcoma (OS) remain largely uncharacterized. The present study investigated the role of miR‑20a in OS cell proliferation. It was determined that miR‑20a expression is markedly upregulated in OS tissues and cells compared with the matched adjacent normal tissues and h‑FOB human osteoblast cell lines. Ectopic expression of miR‑20a promoted the proliferation and anchorage‑independent growth of OS cells, whereas inhibition of miR‑20a reduced this effect. Bioinformatics analysis further revealed early growth response 2 (EGR2), as a potential target of miR‑20a. Data from luciferase reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and translational levels. In functional assays, miR‑20a promoted OS cell proliferation and the cell cycle, which could be suppressed by an inhibitor of miR‑20a. In conclusion, the data provide compelling evidence that miR‑20a functions as an onco‑miRNA, which is important in promoting cell proliferation in OS, and its oncogenic effect is mediated primarily through direct suppression of EGR2 expression.

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Related in: MedlinePlus

EGR-2 upregulation is required for miR-20a-in-induced proliferation of OS cells. (A) Western blot analysis verified that silencing EGR-2 effectively decreased the expression of EGR-2 in miR-20a-in-transfected Saos-2 cells. (B) miR-20a-in-transfected Saos-2 cells after transfection with EGR-2-siRNA promoted cell colony formation. Each bar represents the mean of three independent experiments. *P<0.05, compared with NC. EGR2, early growth response 2; miR, microRNA; OS, osteosarcoma; siRNA, small interfering RNA.
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f6-mmr-12-04-4989: EGR-2 upregulation is required for miR-20a-in-induced proliferation of OS cells. (A) Western blot analysis verified that silencing EGR-2 effectively decreased the expression of EGR-2 in miR-20a-in-transfected Saos-2 cells. (B) miR-20a-in-transfected Saos-2 cells after transfection with EGR-2-siRNA promoted cell colony formation. Each bar represents the mean of three independent experiments. *P<0.05, compared with NC. EGR2, early growth response 2; miR, microRNA; OS, osteosarcoma; siRNA, small interfering RNA.

Mentions: To further investigate the role of EGR2 promotion in miR-20a-in-induced OS proliferation, the effects of EGR2 downregulation on OS cell proliferation were first examined. As predicted, western blot analysis verified that EGR2-siRNA effectively decreased the expression of EGR2 in miR-20a-in-transfected Saos-2 cells (Fig. 6A). Colony formation assays showed that EGR2-silencing and miR-20a-in-transfection in Saos-2 cells exhibited an additive effect on cell proliferation (Fig. 6B). These results demonstrate that direct EGR2 downregulation is required for miR-20a-induced OS cell proliferation.


MicroRNA‑20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing early growth response 2 expression.

Zhuo W, Ge W, Meng G, Jia S, Zhou X, Liu J - Mol Med Rep (2015)

EGR-2 upregulation is required for miR-20a-in-induced proliferation of OS cells. (A) Western blot analysis verified that silencing EGR-2 effectively decreased the expression of EGR-2 in miR-20a-in-transfected Saos-2 cells. (B) miR-20a-in-transfected Saos-2 cells after transfection with EGR-2-siRNA promoted cell colony formation. Each bar represents the mean of three independent experiments. *P<0.05, compared with NC. EGR2, early growth response 2; miR, microRNA; OS, osteosarcoma; siRNA, small interfering RNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581803&req=5

f6-mmr-12-04-4989: EGR-2 upregulation is required for miR-20a-in-induced proliferation of OS cells. (A) Western blot analysis verified that silencing EGR-2 effectively decreased the expression of EGR-2 in miR-20a-in-transfected Saos-2 cells. (B) miR-20a-in-transfected Saos-2 cells after transfection with EGR-2-siRNA promoted cell colony formation. Each bar represents the mean of three independent experiments. *P<0.05, compared with NC. EGR2, early growth response 2; miR, microRNA; OS, osteosarcoma; siRNA, small interfering RNA.
Mentions: To further investigate the role of EGR2 promotion in miR-20a-in-induced OS proliferation, the effects of EGR2 downregulation on OS cell proliferation were first examined. As predicted, western blot analysis verified that EGR2-siRNA effectively decreased the expression of EGR2 in miR-20a-in-transfected Saos-2 cells (Fig. 6A). Colony formation assays showed that EGR2-silencing and miR-20a-in-transfection in Saos-2 cells exhibited an additive effect on cell proliferation (Fig. 6B). These results demonstrate that direct EGR2 downregulation is required for miR-20a-induced OS cell proliferation.

Bottom Line: It was determined that miR‑20a expression is markedly upregulated in OS tissues and cells compared with the matched adjacent normal tissues and h‑FOB human osteoblast cell lines.Data from luciferase reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and translational levels.In conclusion, the data provide compelling evidence that miR‑20a functions as an onco‑miRNA, which is important in promoting cell proliferation in OS, and its oncogenic effect is mediated primarily through direct suppression of EGR2 expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Institute of Orthopedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710000, P.R. China.

ABSTRACT
MicroRNAs (miRNAs) are crucial in cancer development. However, the underlying mechanisms of miRNAs in osteosarcoma (OS) remain largely uncharacterized. The present study investigated the role of miR‑20a in OS cell proliferation. It was determined that miR‑20a expression is markedly upregulated in OS tissues and cells compared with the matched adjacent normal tissues and h‑FOB human osteoblast cell lines. Ectopic expression of miR‑20a promoted the proliferation and anchorage‑independent growth of OS cells, whereas inhibition of miR‑20a reduced this effect. Bioinformatics analysis further revealed early growth response 2 (EGR2), as a potential target of miR‑20a. Data from luciferase reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and translational levels. In functional assays, miR‑20a promoted OS cell proliferation and the cell cycle, which could be suppressed by an inhibitor of miR‑20a. In conclusion, the data provide compelling evidence that miR‑20a functions as an onco‑miRNA, which is important in promoting cell proliferation in OS, and its oncogenic effect is mediated primarily through direct suppression of EGR2 expression.

Show MeSH
Related in: MedlinePlus