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MicroRNA‑20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing early growth response 2 expression.

Zhuo W, Ge W, Meng G, Jia S, Zhou X, Liu J - Mol Med Rep (2015)

Bottom Line: It was determined that miR‑20a expression is markedly upregulated in OS tissues and cells compared with the matched adjacent normal tissues and h‑FOB human osteoblast cell lines.Data from luciferase reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and translational levels.In conclusion, the data provide compelling evidence that miR‑20a functions as an onco‑miRNA, which is important in promoting cell proliferation in OS, and its oncogenic effect is mediated primarily through direct suppression of EGR2 expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Institute of Orthopedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710000, P.R. China.

ABSTRACT
MicroRNAs (miRNAs) are crucial in cancer development. However, the underlying mechanisms of miRNAs in osteosarcoma (OS) remain largely uncharacterized. The present study investigated the role of miR‑20a in OS cell proliferation. It was determined that miR‑20a expression is markedly upregulated in OS tissues and cells compared with the matched adjacent normal tissues and h‑FOB human osteoblast cell lines. Ectopic expression of miR‑20a promoted the proliferation and anchorage‑independent growth of OS cells, whereas inhibition of miR‑20a reduced this effect. Bioinformatics analysis further revealed early growth response 2 (EGR2), as a potential target of miR‑20a. Data from luciferase reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and translational levels. In functional assays, miR‑20a promoted OS cell proliferation and the cell cycle, which could be suppressed by an inhibitor of miR‑20a. In conclusion, the data provide compelling evidence that miR‑20a functions as an onco‑miRNA, which is important in promoting cell proliferation in OS, and its oncogenic effect is mediated primarily through direct suppression of EGR2 expression.

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Related in: MedlinePlus

miR-20a altered the levels of proteins associated with cell proliferation and the cell cycle in Saos-2 cells. (A) Reverse transcription-quantitative polymerase chain reaction analysis of expression of cyclin D1 and p21 in Saos-2 cells. (B) Western blot analysis of cyclin D1 and p21 protein in Saos-2 cells. α-tubulin served as the loading control. *P<0.05, compared with NC. miR, microRNA.
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f5-mmr-12-04-4989: miR-20a altered the levels of proteins associated with cell proliferation and the cell cycle in Saos-2 cells. (A) Reverse transcription-quantitative polymerase chain reaction analysis of expression of cyclin D1 and p21 in Saos-2 cells. (B) Western blot analysis of cyclin D1 and p21 protein in Saos-2 cells. α-tubulin served as the loading control. *P<0.05, compared with NC. miR, microRNA.

Mentions: To investigate the mechanism underlying cell proliferation and the cell cycle, the effect of miR-20a on critical cell-proliferation and cell cycle related regulators cyclin D1 and p21 were analyzed. The cyclin-dependent kinase inhibitor p21 and cyclin D1 are two miR-20a targeted proteins that negatively (p21) and positively (cyclin D1) regulate control cell cycle progression and proliferation (14,15). As shown in Fig. 5, cyclin D1 mRNA and protein were upregulated in Saos-2 cells transfected with miR-20a mimic, but the levels were decreased in the cells transfected with miR-20a-in, relative to control cells. In addition, overexpression of miR-20a caused the downregulation of p21 (mRNA and protein), but upregulation in the cells tranfected with miR-20a-in. Thus providing further evidence that miR-20a is important in OS cell proliferation and the cell cycle.


MicroRNA‑20a promotes the proliferation and cell cycle of human osteosarcoma cells by suppressing early growth response 2 expression.

Zhuo W, Ge W, Meng G, Jia S, Zhou X, Liu J - Mol Med Rep (2015)

miR-20a altered the levels of proteins associated with cell proliferation and the cell cycle in Saos-2 cells. (A) Reverse transcription-quantitative polymerase chain reaction analysis of expression of cyclin D1 and p21 in Saos-2 cells. (B) Western blot analysis of cyclin D1 and p21 protein in Saos-2 cells. α-tubulin served as the loading control. *P<0.05, compared with NC. miR, microRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581803&req=5

f5-mmr-12-04-4989: miR-20a altered the levels of proteins associated with cell proliferation and the cell cycle in Saos-2 cells. (A) Reverse transcription-quantitative polymerase chain reaction analysis of expression of cyclin D1 and p21 in Saos-2 cells. (B) Western blot analysis of cyclin D1 and p21 protein in Saos-2 cells. α-tubulin served as the loading control. *P<0.05, compared with NC. miR, microRNA.
Mentions: To investigate the mechanism underlying cell proliferation and the cell cycle, the effect of miR-20a on critical cell-proliferation and cell cycle related regulators cyclin D1 and p21 were analyzed. The cyclin-dependent kinase inhibitor p21 and cyclin D1 are two miR-20a targeted proteins that negatively (p21) and positively (cyclin D1) regulate control cell cycle progression and proliferation (14,15). As shown in Fig. 5, cyclin D1 mRNA and protein were upregulated in Saos-2 cells transfected with miR-20a mimic, but the levels were decreased in the cells transfected with miR-20a-in, relative to control cells. In addition, overexpression of miR-20a caused the downregulation of p21 (mRNA and protein), but upregulation in the cells tranfected with miR-20a-in. Thus providing further evidence that miR-20a is important in OS cell proliferation and the cell cycle.

Bottom Line: It was determined that miR‑20a expression is markedly upregulated in OS tissues and cells compared with the matched adjacent normal tissues and h‑FOB human osteoblast cell lines.Data from luciferase reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and translational levels.In conclusion, the data provide compelling evidence that miR‑20a functions as an onco‑miRNA, which is important in promoting cell proliferation in OS, and its oncogenic effect is mediated primarily through direct suppression of EGR2 expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Institute of Orthopedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710000, P.R. China.

ABSTRACT
MicroRNAs (miRNAs) are crucial in cancer development. However, the underlying mechanisms of miRNAs in osteosarcoma (OS) remain largely uncharacterized. The present study investigated the role of miR‑20a in OS cell proliferation. It was determined that miR‑20a expression is markedly upregulated in OS tissues and cells compared with the matched adjacent normal tissues and h‑FOB human osteoblast cell lines. Ectopic expression of miR‑20a promoted the proliferation and anchorage‑independent growth of OS cells, whereas inhibition of miR‑20a reduced this effect. Bioinformatics analysis further revealed early growth response 2 (EGR2), as a potential target of miR‑20a. Data from luciferase reporter assays showed that miR‑20a directly binds to the 3'‑untranslated region (3'‑UTR) of EGR2 mRNA and represses expression at the transcriptional and translational levels. In functional assays, miR‑20a promoted OS cell proliferation and the cell cycle, which could be suppressed by an inhibitor of miR‑20a. In conclusion, the data provide compelling evidence that miR‑20a functions as an onco‑miRNA, which is important in promoting cell proliferation in OS, and its oncogenic effect is mediated primarily through direct suppression of EGR2 expression.

Show MeSH
Related in: MedlinePlus