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Girdin regulates the migration and invasion of glioma cells via the PI3K-Akt signaling pathway.

Ni W, Fang Y, Tong L, Tong Z, Yi F, Qiu J, Wang R, Tong X - Mol Med Rep (2015)

Bottom Line: In the present study, short‑hairpin RNA technology was used to silence the gene expression of girdin.In addition, the expression levels and activity of matrix metalloproteinase (MMP)‑2 and MMP‑9 were also affected by girdin silencing.Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy, Histology and Embryology, China Medical University, Shenyang, Liaoning 110001, P.R. China.

ABSTRACT
Girdin, an actin‑binding protein, is associated with cell migration and is expressed at high levels in glioma cells. However, the association between girdin and the development of glioma remains to be elucidated. In the present study, short‑hairpin RNA technology was used to silence the gene expression of girdin. The effects of girdin silencing on glioma cell proliferation, migration and invasion were then assessed using a cell viability assay, wound‑healing assay, transwell invasion assay, reverse transcription‑quantitative polymerase chain reaction, western blot analysis and gelatin zymography. The results suggested that girdin silencing inhibited the proliferation, migration and invasion of glioma cells. In addition, the expression levels and activity of matrix metalloproteinase (MMP)‑2 and MMP‑9 were also affected by girdin silencing. Further mechanistic investigation indicated that girdin may regulate glioma cell migration and invasion through the phosphatidylinositol‑3‑kinase/protein kinase B (PI3K‑Akt) signaling pathway. Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs.

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Related in: MedlinePlus

Girdin silencing inhibits the proliferation, migration and invasion of glioma cells. (A) Changes of cell viability were detected using a 3-(4,5-dimethy -2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay following transfection. (B and C) Following transfection, a wound-healing assay was performed to investigate changes in migration capacity (scale bar, 100 µm). (D and E) A Transwell invasion assay was performed to detect cell invasion capacity following transfection. Images were captured under a light microscope (magnification, ×200). Each experiment was repeated three times. The experimental results are presented as the means ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001, compared with the NC group. shRNA, short hairpin RNA; NC, negative control.
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f2-mmr-12-04-5086: Girdin silencing inhibits the proliferation, migration and invasion of glioma cells. (A) Changes of cell viability were detected using a 3-(4,5-dimethy -2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay following transfection. (B and C) Following transfection, a wound-healing assay was performed to investigate changes in migration capacity (scale bar, 100 µm). (D and E) A Transwell invasion assay was performed to detect cell invasion capacity following transfection. Images were captured under a light microscope (magnification, ×200). Each experiment was repeated three times. The experimental results are presented as the means ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001, compared with the NC group. shRNA, short hairpin RNA; NC, negative control.

Mentions: To evaluate the effect of girdin silencing on glioma proliferation, migration and invasion, an MTT assay was used to determine the changes in cell viability following transfection with girdin shRNA. The MTT assay results demonstrated that girdin silencing significantly reduced the proliferation of glioma cells (P<0.01; Fig. 2A). A wound-healing assay was subsequently performed to detect changes in migration capacity of the cells. The results of the wound-healing assay demonstrated that the relative mobility of the glioma cells was significantly lower following girdin silencing, compared with that of the cells transfected with NC (P<0.05; Fig. 2B and C), suggesting that girdin silencing may impede cell migration. In addition, Transwell invasion assays were also performed to evaluate the effect of girdin silencing on glioma cell invasion. The results revealed that fewer cells passed through the microporous membrane following transfection with girdin shRNA (P<0.001; Fig. 2D and E), indicating that girdin silencing significantly reduced glioma cell invasion. The above results suggested that girdin silencing suppressed glioma cell proliferation, migration and invasion.


Girdin regulates the migration and invasion of glioma cells via the PI3K-Akt signaling pathway.

Ni W, Fang Y, Tong L, Tong Z, Yi F, Qiu J, Wang R, Tong X - Mol Med Rep (2015)

Girdin silencing inhibits the proliferation, migration and invasion of glioma cells. (A) Changes of cell viability were detected using a 3-(4,5-dimethy -2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay following transfection. (B and C) Following transfection, a wound-healing assay was performed to investigate changes in migration capacity (scale bar, 100 µm). (D and E) A Transwell invasion assay was performed to detect cell invasion capacity following transfection. Images were captured under a light microscope (magnification, ×200). Each experiment was repeated three times. The experimental results are presented as the means ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001, compared with the NC group. shRNA, short hairpin RNA; NC, negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581799&req=5

f2-mmr-12-04-5086: Girdin silencing inhibits the proliferation, migration and invasion of glioma cells. (A) Changes of cell viability were detected using a 3-(4,5-dimethy -2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay following transfection. (B and C) Following transfection, a wound-healing assay was performed to investigate changes in migration capacity (scale bar, 100 µm). (D and E) A Transwell invasion assay was performed to detect cell invasion capacity following transfection. Images were captured under a light microscope (magnification, ×200). Each experiment was repeated three times. The experimental results are presented as the means ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001, compared with the NC group. shRNA, short hairpin RNA; NC, negative control.
Mentions: To evaluate the effect of girdin silencing on glioma proliferation, migration and invasion, an MTT assay was used to determine the changes in cell viability following transfection with girdin shRNA. The MTT assay results demonstrated that girdin silencing significantly reduced the proliferation of glioma cells (P<0.01; Fig. 2A). A wound-healing assay was subsequently performed to detect changes in migration capacity of the cells. The results of the wound-healing assay demonstrated that the relative mobility of the glioma cells was significantly lower following girdin silencing, compared with that of the cells transfected with NC (P<0.05; Fig. 2B and C), suggesting that girdin silencing may impede cell migration. In addition, Transwell invasion assays were also performed to evaluate the effect of girdin silencing on glioma cell invasion. The results revealed that fewer cells passed through the microporous membrane following transfection with girdin shRNA (P<0.001; Fig. 2D and E), indicating that girdin silencing significantly reduced glioma cell invasion. The above results suggested that girdin silencing suppressed glioma cell proliferation, migration and invasion.

Bottom Line: In the present study, short‑hairpin RNA technology was used to silence the gene expression of girdin.In addition, the expression levels and activity of matrix metalloproteinase (MMP)‑2 and MMP‑9 were also affected by girdin silencing.Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy, Histology and Embryology, China Medical University, Shenyang, Liaoning 110001, P.R. China.

ABSTRACT
Girdin, an actin‑binding protein, is associated with cell migration and is expressed at high levels in glioma cells. However, the association between girdin and the development of glioma remains to be elucidated. In the present study, short‑hairpin RNA technology was used to silence the gene expression of girdin. The effects of girdin silencing on glioma cell proliferation, migration and invasion were then assessed using a cell viability assay, wound‑healing assay, transwell invasion assay, reverse transcription‑quantitative polymerase chain reaction, western blot analysis and gelatin zymography. The results suggested that girdin silencing inhibited the proliferation, migration and invasion of glioma cells. In addition, the expression levels and activity of matrix metalloproteinase (MMP)‑2 and MMP‑9 were also affected by girdin silencing. Further mechanistic investigation indicated that girdin may regulate glioma cell migration and invasion through the phosphatidylinositol‑3‑kinase/protein kinase B (PI3K‑Akt) signaling pathway. Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs.

Show MeSH
Related in: MedlinePlus