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Prostaglandin E₁ protects bone marrow-derived mesenchymal stem cells against serum deprivation-induced apoptosis.

Zeng K, Deng BP, Jiang HQ, Wang M, Hua P, Zhang HW, Deng YB, Yang YQ - Mol Med Rep (2015)

Bottom Line: Prostaglandin E1 (PGE1) is known to have anti‑inflammatory and anti‑apoptotic effects on the myocardium.The results of the present study demonstrated that SD induced apoptosis of MSCs, and that treatment with PGE1 attenuated the morphological changes characteristic of apoptosis.Furthermore, treatment with PGE1 significantly reduced SD‑induced apoptosis, decreased the protein expression levels of Bax and caspase‑3, and increased the expression levels of Bcl‑2.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiac Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China.

ABSTRACT
Mesenchymal stem cells (MSCs) have become a recent focus of experimental and clinical research regarding myocardial regeneration. However, the therapeutic potential of these cells is limited by poor survival. Prostaglandin E1 (PGE1) is known to have anti‑inflammatory and anti‑apoptotic effects on the myocardium. The aim of the present study was to determine whether PGE1 could protect MSCs against serum deprivation (SD)‑induced apoptosis. An SD model was used to induce apoptosis in MSCs in vitro. Apoptotic morphological changes were detected by Hoechst 33258 fluorescent nuclear staining; and Annexin V‑fluorescein isothiocyanate/propidium iodide (PI) double staining and flow cytometry was used to quantify the rate of apoptosis. Western blot analysis was used to detect the expression levels of the apoptosis‑associated proteins Bcl‑2, Bax and caspase‑3. The results of the present study demonstrated that SD induced apoptosis of MSCs, and that treatment with PGE1 attenuated the morphological changes characteristic of apoptosis. Annexin V/PI staining showed that the rate of apoptosis gradually increased with the duration of ischemia. Furthermore, treatment with PGE1 significantly reduced SD‑induced apoptosis, decreased the protein expression levels of Bax and caspase‑3, and increased the expression levels of Bcl‑2. These data suggest that PGE1 is able to influence the survival of MSCs under certain conditions. These results may aid in improving the therapeutic efficacy of MSC transplantation used to treat chronic ischemic heart disease.

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Related in: MedlinePlus

Protein expression levels of Bcl-2, Bax and caspase-3 in MSCs, as determined by western blotting following 12, 24 and 48 h of serum deprivation. Protein expression levels of (A) Bcl-2, (B) Bax and (C) cleaved caspase-3. GAPDH was used as a loading control, and the expression levels of the target proteins were determined relative to the levels of GAPDH. Blots shown are from ≥three independent experiments. The data are presented as the mean ± standard error (n=5). *P<0.05 and **P<0.01, as compared with the control group. MSCs, mesenchymal stem cells; Con, control; SD, serum deprived.
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f3-mmr-12-04-5723: Protein expression levels of Bcl-2, Bax and caspase-3 in MSCs, as determined by western blotting following 12, 24 and 48 h of serum deprivation. Protein expression levels of (A) Bcl-2, (B) Bax and (C) cleaved caspase-3. GAPDH was used as a loading control, and the expression levels of the target proteins were determined relative to the levels of GAPDH. Blots shown are from ≥three independent experiments. The data are presented as the mean ± standard error (n=5). *P<0.05 and **P<0.01, as compared with the control group. MSCs, mesenchymal stem cells; Con, control; SD, serum deprived.

Mentions: The present study determined whether SD could affect the expression levels of the proapoptotic Bcl-2 family members, Bax and Bcl-2. Western blot analysis showed that the protein expression levels of Bax were significantly increased and the protein expression levels of Bcl-2 gradually decreased, following exposure to SD for 12–24 h (Fig. 3A and B). Cleaved caspase-3 expression was not detected in the control MSCs, however its expression was significantly increased in the MSCs cultured in SD medium (Fig. 3C).


Prostaglandin E₁ protects bone marrow-derived mesenchymal stem cells against serum deprivation-induced apoptosis.

Zeng K, Deng BP, Jiang HQ, Wang M, Hua P, Zhang HW, Deng YB, Yang YQ - Mol Med Rep (2015)

Protein expression levels of Bcl-2, Bax and caspase-3 in MSCs, as determined by western blotting following 12, 24 and 48 h of serum deprivation. Protein expression levels of (A) Bcl-2, (B) Bax and (C) cleaved caspase-3. GAPDH was used as a loading control, and the expression levels of the target proteins were determined relative to the levels of GAPDH. Blots shown are from ≥three independent experiments. The data are presented as the mean ± standard error (n=5). *P<0.05 and **P<0.01, as compared with the control group. MSCs, mesenchymal stem cells; Con, control; SD, serum deprived.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581785&req=5

f3-mmr-12-04-5723: Protein expression levels of Bcl-2, Bax and caspase-3 in MSCs, as determined by western blotting following 12, 24 and 48 h of serum deprivation. Protein expression levels of (A) Bcl-2, (B) Bax and (C) cleaved caspase-3. GAPDH was used as a loading control, and the expression levels of the target proteins were determined relative to the levels of GAPDH. Blots shown are from ≥three independent experiments. The data are presented as the mean ± standard error (n=5). *P<0.05 and **P<0.01, as compared with the control group. MSCs, mesenchymal stem cells; Con, control; SD, serum deprived.
Mentions: The present study determined whether SD could affect the expression levels of the proapoptotic Bcl-2 family members, Bax and Bcl-2. Western blot analysis showed that the protein expression levels of Bax were significantly increased and the protein expression levels of Bcl-2 gradually decreased, following exposure to SD for 12–24 h (Fig. 3A and B). Cleaved caspase-3 expression was not detected in the control MSCs, however its expression was significantly increased in the MSCs cultured in SD medium (Fig. 3C).

Bottom Line: Prostaglandin E1 (PGE1) is known to have anti‑inflammatory and anti‑apoptotic effects on the myocardium.The results of the present study demonstrated that SD induced apoptosis of MSCs, and that treatment with PGE1 attenuated the morphological changes characteristic of apoptosis.Furthermore, treatment with PGE1 significantly reduced SD‑induced apoptosis, decreased the protein expression levels of Bax and caspase‑3, and increased the expression levels of Bcl‑2.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiac Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China.

ABSTRACT
Mesenchymal stem cells (MSCs) have become a recent focus of experimental and clinical research regarding myocardial regeneration. However, the therapeutic potential of these cells is limited by poor survival. Prostaglandin E1 (PGE1) is known to have anti‑inflammatory and anti‑apoptotic effects on the myocardium. The aim of the present study was to determine whether PGE1 could protect MSCs against serum deprivation (SD)‑induced apoptosis. An SD model was used to induce apoptosis in MSCs in vitro. Apoptotic morphological changes were detected by Hoechst 33258 fluorescent nuclear staining; and Annexin V‑fluorescein isothiocyanate/propidium iodide (PI) double staining and flow cytometry was used to quantify the rate of apoptosis. Western blot analysis was used to detect the expression levels of the apoptosis‑associated proteins Bcl‑2, Bax and caspase‑3. The results of the present study demonstrated that SD induced apoptosis of MSCs, and that treatment with PGE1 attenuated the morphological changes characteristic of apoptosis. Annexin V/PI staining showed that the rate of apoptosis gradually increased with the duration of ischemia. Furthermore, treatment with PGE1 significantly reduced SD‑induced apoptosis, decreased the protein expression levels of Bax and caspase‑3, and increased the expression levels of Bcl‑2. These data suggest that PGE1 is able to influence the survival of MSCs under certain conditions. These results may aid in improving the therapeutic efficacy of MSC transplantation used to treat chronic ischemic heart disease.

Show MeSH
Related in: MedlinePlus