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Association between xanthine dehydrogenase tag single nucleotide polymorphisms and essential hypertension.

Wu B, Hao Y, Shi J, Geng N, Li T, Chen Y, Sun Z, Zheng L, Li H, Li N, Zhang X, Sun Y - Mol Med Rep (2015)

Bottom Line: Han Chinese individuals, who had lived in rural areas of Fuxin, were selected as subjects for the present study.Five tag single nucleotide polymorphisms (SNP) of the XDH gene were selected.These results suggest that the XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 may be associated with hypertension in the rural Han Chinese population.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.

ABSTRACT
The present study aimed to investigate the association between xanthine dehydrogenase (XDH) gene polymorphism and essential hypertension in the rural Han Chinese population of Fuxin, Liaoning. Han Chinese individuals, who had lived in rural areas of Fuxin, were selected as subjects for the present study. A total of 521 unrelated patients with hypertension were selected, along with a further 533 unrelated individuals with normal blood pressure, in order to serve as controls. Five tag single nucleotide polymorphisms (SNP) of the XDH gene were selected. An estimation of SNP allele frequency was determined using DNA pooling and pyrosequencing methods. Prior to Bonferroni correction, T allele frequency for rs206811 was significantly higher in patients with hypertension, as compared with the controls (64.1 vs. 59.4%; P=0.031); C allele frequency for rs1042039 was significantly higher in patients with hypertension, as compared with the controls (66.1 vs. 60.6%; P=0.011), C allele frequency for rs1054889 was significantly lower in patients with hypertension, as compared with the controls (38.8 vs. 44.8%; P=0.007); and A allele frequency for rs2073316 was significantly lower in patients with hypertension, as compared with the controls (29.2 vs. 34.4%; P=0.013). However, once a Bonferroni correction for multiple testing was applied, the XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 were shown to be associated with hypertension (P=0.044, 0.035, and 0.039, respectively). These results suggest that the XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 may be associated with hypertension in the rural Han Chinese population.

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rs1042039 case group 1 peak value.
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f4-mmr-12-04-5685: rs1042039 case group 1 peak value.

Mentions: For each pooled sample, the peak height of the SNP alleles was determined using the PSQ96 AQ software, and the ratio of one allele peak height to the sum of the heights of both allele peaks was calculated. Peak values of five SNPs of controls and hypertensives were shown in Figs. 1–10. Using the standard curve, the SNP allele peak height ratios were converted to allele frequencies in the DNA pools. Allele frequency for each DNA pool was calculated as the mean value of the three replicates of each pooled sample. Using the previously determined allele frequencies of each DNA pool, the mean value of five DNA pools was then calculated in order to represent the allele frequency in hypertensives and controls. Prior to the application of Bonferroni correction, T allele frequency for rs206811 was significantly higher in hypertensives, as compared with controls (64.1 vs. 59.4%, P=0.031); C allele frequency for rs1042039 was significantly higher in hypertensives, as compared with controls (66.1 vs. 60.6%, P=0.011), C allele frequency for rs1054889 was significantly lower in hypertensives, as compared with controls (38.8 vs. 44.8%, P=0.007); and A allele frequency for rs2073316 was significantly lower in hypertensives, as compared with the controls (29.2 vs. 34.4%, P=0.013). Following Bonferroni correction for multiple testing, the rs1042039, rs1054889, and rs2073316 polymorphisms of the XDH gene were associated with hypertension (P=0.044, 0.035, and 0.039, respectively). All results are shown in Table III.


Association between xanthine dehydrogenase tag single nucleotide polymorphisms and essential hypertension.

Wu B, Hao Y, Shi J, Geng N, Li T, Chen Y, Sun Z, Zheng L, Li H, Li N, Zhang X, Sun Y - Mol Med Rep (2015)

rs1042039 case group 1 peak value.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581766&req=5

f4-mmr-12-04-5685: rs1042039 case group 1 peak value.
Mentions: For each pooled sample, the peak height of the SNP alleles was determined using the PSQ96 AQ software, and the ratio of one allele peak height to the sum of the heights of both allele peaks was calculated. Peak values of five SNPs of controls and hypertensives were shown in Figs. 1–10. Using the standard curve, the SNP allele peak height ratios were converted to allele frequencies in the DNA pools. Allele frequency for each DNA pool was calculated as the mean value of the three replicates of each pooled sample. Using the previously determined allele frequencies of each DNA pool, the mean value of five DNA pools was then calculated in order to represent the allele frequency in hypertensives and controls. Prior to the application of Bonferroni correction, T allele frequency for rs206811 was significantly higher in hypertensives, as compared with controls (64.1 vs. 59.4%, P=0.031); C allele frequency for rs1042039 was significantly higher in hypertensives, as compared with controls (66.1 vs. 60.6%, P=0.011), C allele frequency for rs1054889 was significantly lower in hypertensives, as compared with controls (38.8 vs. 44.8%, P=0.007); and A allele frequency for rs2073316 was significantly lower in hypertensives, as compared with the controls (29.2 vs. 34.4%, P=0.013). Following Bonferroni correction for multiple testing, the rs1042039, rs1054889, and rs2073316 polymorphisms of the XDH gene were associated with hypertension (P=0.044, 0.035, and 0.039, respectively). All results are shown in Table III.

Bottom Line: Han Chinese individuals, who had lived in rural areas of Fuxin, were selected as subjects for the present study.Five tag single nucleotide polymorphisms (SNP) of the XDH gene were selected.These results suggest that the XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 may be associated with hypertension in the rural Han Chinese population.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.

ABSTRACT
The present study aimed to investigate the association between xanthine dehydrogenase (XDH) gene polymorphism and essential hypertension in the rural Han Chinese population of Fuxin, Liaoning. Han Chinese individuals, who had lived in rural areas of Fuxin, were selected as subjects for the present study. A total of 521 unrelated patients with hypertension were selected, along with a further 533 unrelated individuals with normal blood pressure, in order to serve as controls. Five tag single nucleotide polymorphisms (SNP) of the XDH gene were selected. An estimation of SNP allele frequency was determined using DNA pooling and pyrosequencing methods. Prior to Bonferroni correction, T allele frequency for rs206811 was significantly higher in patients with hypertension, as compared with the controls (64.1 vs. 59.4%; P=0.031); C allele frequency for rs1042039 was significantly higher in patients with hypertension, as compared with the controls (66.1 vs. 60.6%; P=0.011), C allele frequency for rs1054889 was significantly lower in patients with hypertension, as compared with the controls (38.8 vs. 44.8%; P=0.007); and A allele frequency for rs2073316 was significantly lower in patients with hypertension, as compared with the controls (29.2 vs. 34.4%; P=0.013). However, once a Bonferroni correction for multiple testing was applied, the XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 were shown to be associated with hypertension (P=0.044, 0.035, and 0.039, respectively). These results suggest that the XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 may be associated with hypertension in the rural Han Chinese population.

Show MeSH
Related in: MedlinePlus