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Propofol suppresses proliferation and invasion of glioma cells by upregulating microRNA-218 expression.

Xu J, Xu W, Zhu J - Mol Med Rep (2015)

Bottom Line: Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent.In addition, treatment with propofol efficiently reduced MMP‑2 protein expression levels, and overexpression of miR‑218 also decreased MMP‑2 protein expression levels.Whereas, neutralization of miR‑218 using the anti‑miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Jinhua Municipal Central Hospital, Jinhua, Zhejiang 321000, P.R. China.

ABSTRACT
Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent. The present study aimed to assess the effect of propofol on the proliferation and invasion of human glioma cells, and to determine the potential underlying molecular mechanisms. The effects of propofol on U373 glioblastoma cell proliferation, apoptosis and invasion were detected by an MTT assay, caspase‑3 activity measurement and a Matrigel™ invasion assay, respectively. MicroRNA (miR)‑218 expression and matrix metalloproteinase (MMP)‑2 protein expression levels were analyzed by quantitative polymerase chain reaction and western blot analysis, respectively. In addition, miR‑218 precursor was transfected into the cells to assess whether overexpression of miR‑218 could affect MMP‑2 expression. Anti‑miR‑218 was transfected into the cells to evaluate the role of miR‑218 in the effects of propofol on the biological behavior of glioma cells. The results of the present study demonstrated that propofol significantly increased the expression levels of miR‑218, inhibited U373 cell proliferation and invasion, and facilitated apoptosis. In addition, treatment with propofol efficiently reduced MMP‑2 protein expression levels, and overexpression of miR‑218 also decreased MMP‑2 protein expression levels. Whereas, neutralization of miR‑218 using the anti‑miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression. In conclusion, propofol may effectively suppress proliferation and invasion, and induce the apoptosis of glioma cells, at least partially through upregulation of miR-218 expression.

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Propofol stimulates microRNA (miR)-218 expression in U373 human glioma cells. Propofol treatment increased the expression levels of miR-218 in a dose-dependent manner. *P<0.01, compared with the control untreated group.
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f3-mmr-12-04-4815: Propofol stimulates microRNA (miR)-218 expression in U373 human glioma cells. Propofol treatment increased the expression levels of miR-218 in a dose-dependent manner. *P<0.01, compared with the control untreated group.

Mentions: Treatment with propofol dose-dependently augmented miR-218 expression in U373 cells (Fig. 3). Specifically, treatment with 5μg/ml propofol enhanced the miR-218 expression in U373 cells 2.19 fold and 10 μg/ml propofol elevated the miR-218 expression in U373 cells 3.07 fold.


Propofol suppresses proliferation and invasion of glioma cells by upregulating microRNA-218 expression.

Xu J, Xu W, Zhu J - Mol Med Rep (2015)

Propofol stimulates microRNA (miR)-218 expression in U373 human glioma cells. Propofol treatment increased the expression levels of miR-218 in a dose-dependent manner. *P<0.01, compared with the control untreated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581763&req=5

f3-mmr-12-04-4815: Propofol stimulates microRNA (miR)-218 expression in U373 human glioma cells. Propofol treatment increased the expression levels of miR-218 in a dose-dependent manner. *P<0.01, compared with the control untreated group.
Mentions: Treatment with propofol dose-dependently augmented miR-218 expression in U373 cells (Fig. 3). Specifically, treatment with 5μg/ml propofol enhanced the miR-218 expression in U373 cells 2.19 fold and 10 μg/ml propofol elevated the miR-218 expression in U373 cells 3.07 fold.

Bottom Line: Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent.In addition, treatment with propofol efficiently reduced MMP‑2 protein expression levels, and overexpression of miR‑218 also decreased MMP‑2 protein expression levels.Whereas, neutralization of miR‑218 using the anti‑miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Jinhua Municipal Central Hospital, Jinhua, Zhejiang 321000, P.R. China.

ABSTRACT
Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent. The present study aimed to assess the effect of propofol on the proliferation and invasion of human glioma cells, and to determine the potential underlying molecular mechanisms. The effects of propofol on U373 glioblastoma cell proliferation, apoptosis and invasion were detected by an MTT assay, caspase‑3 activity measurement and a Matrigel™ invasion assay, respectively. MicroRNA (miR)‑218 expression and matrix metalloproteinase (MMP)‑2 protein expression levels were analyzed by quantitative polymerase chain reaction and western blot analysis, respectively. In addition, miR‑218 precursor was transfected into the cells to assess whether overexpression of miR‑218 could affect MMP‑2 expression. Anti‑miR‑218 was transfected into the cells to evaluate the role of miR‑218 in the effects of propofol on the biological behavior of glioma cells. The results of the present study demonstrated that propofol significantly increased the expression levels of miR‑218, inhibited U373 cell proliferation and invasion, and facilitated apoptosis. In addition, treatment with propofol efficiently reduced MMP‑2 protein expression levels, and overexpression of miR‑218 also decreased MMP‑2 protein expression levels. Whereas, neutralization of miR‑218 using the anti‑miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression. In conclusion, propofol may effectively suppress proliferation and invasion, and induce the apoptosis of glioma cells, at least partially through upregulation of miR-218 expression.

Show MeSH
Related in: MedlinePlus