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Propofol suppresses proliferation and invasion of glioma cells by upregulating microRNA-218 expression.

Xu J, Xu W, Zhu J - Mol Med Rep (2015)

Bottom Line: Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent.In addition, treatment with propofol efficiently reduced MMP‑2 protein expression levels, and overexpression of miR‑218 also decreased MMP‑2 protein expression levels.Whereas, neutralization of miR‑218 using the anti‑miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Jinhua Municipal Central Hospital, Jinhua, Zhejiang 321000, P.R. China.

ABSTRACT
Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent. The present study aimed to assess the effect of propofol on the proliferation and invasion of human glioma cells, and to determine the potential underlying molecular mechanisms. The effects of propofol on U373 glioblastoma cell proliferation, apoptosis and invasion were detected by an MTT assay, caspase‑3 activity measurement and a Matrigel™ invasion assay, respectively. MicroRNA (miR)‑218 expression and matrix metalloproteinase (MMP)‑2 protein expression levels were analyzed by quantitative polymerase chain reaction and western blot analysis, respectively. In addition, miR‑218 precursor was transfected into the cells to assess whether overexpression of miR‑218 could affect MMP‑2 expression. Anti‑miR‑218 was transfected into the cells to evaluate the role of miR‑218 in the effects of propofol on the biological behavior of glioma cells. The results of the present study demonstrated that propofol significantly increased the expression levels of miR‑218, inhibited U373 cell proliferation and invasion, and facilitated apoptosis. In addition, treatment with propofol efficiently reduced MMP‑2 protein expression levels, and overexpression of miR‑218 also decreased MMP‑2 protein expression levels. Whereas, neutralization of miR‑218 using the anti‑miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression. In conclusion, propofol may effectively suppress proliferation and invasion, and induce the apoptosis of glioma cells, at least partially through upregulation of miR-218 expression.

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Effects of propofol on cell proliferation, apoptosis and invasion. (A) The MTT assay showed that the proliferation of U373 cells was markedly suppressed by propofol in a dose- and time-dependent manner. (B) Hoechst 33258 staining demonstrated promoted U373 cell apoptosis following propofol stimulation. (C) High caspase-3 activity was observed following propofol treatment. (D) A Matrigel invasion assay showed a significant decrease in the number of invaded cell numbers following propofol stimulation. *P<0.01, compared with the untreated control group.
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f1-mmr-12-04-4815: Effects of propofol on cell proliferation, apoptosis and invasion. (A) The MTT assay showed that the proliferation of U373 cells was markedly suppressed by propofol in a dose- and time-dependent manner. (B) Hoechst 33258 staining demonstrated promoted U373 cell apoptosis following propofol stimulation. (C) High caspase-3 activity was observed following propofol treatment. (D) A Matrigel invasion assay showed a significant decrease in the number of invaded cell numbers following propofol stimulation. *P<0.01, compared with the untreated control group.

Mentions: The present study aimed to determine the effects of propofol on proliferation, apoptosis and invasion in the U373 glioma cell line. Following treatment with various concentrations of propofol, the proliferation of U373 cells was measured using the MTT method. The proliferation of U373 cells was markedly suppressed by propofol in a dose- and time-dependent manner (Fig. 1A). Treatment with propofol at 5 and 10 μg/ml significantly inhibited the proliferation of the cells at 48 and 72 h. Furthermore, the rate of cell apoptosis was evaluated by Hoechst 33258 staining and caspase-3 activity measurement. The U373 cells had an increased rate of apoptosis following treatment with propofol for 48 h (Fig. 1B and C). A Matrigel™ invasion assay also demonstrated that propofol significantly reduced cell invasion when the cells were treated with 5 and 10 μg/ml (Fig. 1D). These results indicate that propofol inhibits proliferation and invasion, and promotes apoptosis of U373 cells.


Propofol suppresses proliferation and invasion of glioma cells by upregulating microRNA-218 expression.

Xu J, Xu W, Zhu J - Mol Med Rep (2015)

Effects of propofol on cell proliferation, apoptosis and invasion. (A) The MTT assay showed that the proliferation of U373 cells was markedly suppressed by propofol in a dose- and time-dependent manner. (B) Hoechst 33258 staining demonstrated promoted U373 cell apoptosis following propofol stimulation. (C) High caspase-3 activity was observed following propofol treatment. (D) A Matrigel invasion assay showed a significant decrease in the number of invaded cell numbers following propofol stimulation. *P<0.01, compared with the untreated control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581763&req=5

f1-mmr-12-04-4815: Effects of propofol on cell proliferation, apoptosis and invasion. (A) The MTT assay showed that the proliferation of U373 cells was markedly suppressed by propofol in a dose- and time-dependent manner. (B) Hoechst 33258 staining demonstrated promoted U373 cell apoptosis following propofol stimulation. (C) High caspase-3 activity was observed following propofol treatment. (D) A Matrigel invasion assay showed a significant decrease in the number of invaded cell numbers following propofol stimulation. *P<0.01, compared with the untreated control group.
Mentions: The present study aimed to determine the effects of propofol on proliferation, apoptosis and invasion in the U373 glioma cell line. Following treatment with various concentrations of propofol, the proliferation of U373 cells was measured using the MTT method. The proliferation of U373 cells was markedly suppressed by propofol in a dose- and time-dependent manner (Fig. 1A). Treatment with propofol at 5 and 10 μg/ml significantly inhibited the proliferation of the cells at 48 and 72 h. Furthermore, the rate of cell apoptosis was evaluated by Hoechst 33258 staining and caspase-3 activity measurement. The U373 cells had an increased rate of apoptosis following treatment with propofol for 48 h (Fig. 1B and C). A Matrigel™ invasion assay also demonstrated that propofol significantly reduced cell invasion when the cells were treated with 5 and 10 μg/ml (Fig. 1D). These results indicate that propofol inhibits proliferation and invasion, and promotes apoptosis of U373 cells.

Bottom Line: Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent.In addition, treatment with propofol efficiently reduced MMP‑2 protein expression levels, and overexpression of miR‑218 also decreased MMP‑2 protein expression levels.Whereas, neutralization of miR‑218 using the anti‑miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Jinhua Municipal Central Hospital, Jinhua, Zhejiang 321000, P.R. China.

ABSTRACT
Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent. The present study aimed to assess the effect of propofol on the proliferation and invasion of human glioma cells, and to determine the potential underlying molecular mechanisms. The effects of propofol on U373 glioblastoma cell proliferation, apoptosis and invasion were detected by an MTT assay, caspase‑3 activity measurement and a Matrigel™ invasion assay, respectively. MicroRNA (miR)‑218 expression and matrix metalloproteinase (MMP)‑2 protein expression levels were analyzed by quantitative polymerase chain reaction and western blot analysis, respectively. In addition, miR‑218 precursor was transfected into the cells to assess whether overexpression of miR‑218 could affect MMP‑2 expression. Anti‑miR‑218 was transfected into the cells to evaluate the role of miR‑218 in the effects of propofol on the biological behavior of glioma cells. The results of the present study demonstrated that propofol significantly increased the expression levels of miR‑218, inhibited U373 cell proliferation and invasion, and facilitated apoptosis. In addition, treatment with propofol efficiently reduced MMP‑2 protein expression levels, and overexpression of miR‑218 also decreased MMP‑2 protein expression levels. Whereas, neutralization of miR‑218 using the anti‑miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression. In conclusion, propofol may effectively suppress proliferation and invasion, and induce the apoptosis of glioma cells, at least partially through upregulation of miR-218 expression.

Show MeSH
Related in: MedlinePlus