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MicroRNA‑205 promotes the tumorigenesis of nasopharyngeal carcinoma through targeting tumor protein p53-inducible nuclear protein 1.

Nie G, Duan H, Li X, Yu Z, Luo L, Lu R, Ji Z, Zhang W - Mol Med Rep (2015)

Bottom Line: The present study aimed to explore the potential role of miR‑205 in NPC.The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues.Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

ABSTRACT
Nasopharyngeal carcinoma (NPC) is a common type of cancer in southern China, miRNAs have been shown to be involved in the tumorigenesis of multiple cancer types. The present study aimed to explore the potential role of miR‑205 in NPC. Reverse transcription quantitative polymerase chain reaction was used to determine the expression levels of miR‑205 in 20 fresh NPC specimens and 20 normal nasopharyngeal tissues. The function of miR‑205 in the proliferation, migration, invasion and apoptosis of NPC‑derived cells was detected by MTT assay, colony formation assay, wound healing assay, Transwell assay and flow cytometry. Furthermore, a target gene of miR‑205 was identified using the luciferase reporter assay. The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues. Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed. Tumor protein p53-inducible nuclear protein 1 was identified as a target gene of miR‑205. Overall, the present study demonstrated that miR‑205 may function as an oncogene in NPC tumorigenesis.

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Related in: MedlinePlus

Effects of miR-205 on cell migration and invasion of NPC cells. (A) Cell migration was determined by a wound healing assay. The promotion rates of cell migration were 56.8% in CNE2 cells (P=0.003), 108.6% in 6-10B cells (P=0.003) and 95.2% in 9-4E cells (P=0.004) after transfection with miR-205. (B) Cell invasion was determined by a Transwell assay. The number of invaded cells was increased in CNE2, 6-10B and 9-4E cells by 61.4% (P=0.000), 67.4% (P=0.008) and 86.6% (P=0.003), respectively, after transfection with miR-205. Cells are stained with crystal violet; magnification, ×10. Values are expressed as the mean ± standard deviation. *P<0.05; **P<0.001 vs. NC. miR, microRNA; NC, negative control; NPC, nasopharyngeal carcinoma.
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f5-mmr-12-04-5715: Effects of miR-205 on cell migration and invasion of NPC cells. (A) Cell migration was determined by a wound healing assay. The promotion rates of cell migration were 56.8% in CNE2 cells (P=0.003), 108.6% in 6-10B cells (P=0.003) and 95.2% in 9-4E cells (P=0.004) after transfection with miR-205. (B) Cell invasion was determined by a Transwell assay. The number of invaded cells was increased in CNE2, 6-10B and 9-4E cells by 61.4% (P=0.000), 67.4% (P=0.008) and 86.6% (P=0.003), respectively, after transfection with miR-205. Cells are stained with crystal violet; magnification, ×10. Values are expressed as the mean ± standard deviation. *P<0.05; **P<0.001 vs. NC. miR, microRNA; NC, negative control; NPC, nasopharyngeal carcinoma.

Mentions: The wound-healing and invasion assays indicated that overexpression of miR-205 increased the rates of migration and invasion of CNE2, 6-10B and 9-4E cells compared with those of the negative control cells (Fig. 5A and B).


MicroRNA‑205 promotes the tumorigenesis of nasopharyngeal carcinoma through targeting tumor protein p53-inducible nuclear protein 1.

Nie G, Duan H, Li X, Yu Z, Luo L, Lu R, Ji Z, Zhang W - Mol Med Rep (2015)

Effects of miR-205 on cell migration and invasion of NPC cells. (A) Cell migration was determined by a wound healing assay. The promotion rates of cell migration were 56.8% in CNE2 cells (P=0.003), 108.6% in 6-10B cells (P=0.003) and 95.2% in 9-4E cells (P=0.004) after transfection with miR-205. (B) Cell invasion was determined by a Transwell assay. The number of invaded cells was increased in CNE2, 6-10B and 9-4E cells by 61.4% (P=0.000), 67.4% (P=0.008) and 86.6% (P=0.003), respectively, after transfection with miR-205. Cells are stained with crystal violet; magnification, ×10. Values are expressed as the mean ± standard deviation. *P<0.05; **P<0.001 vs. NC. miR, microRNA; NC, negative control; NPC, nasopharyngeal carcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581759&req=5

f5-mmr-12-04-5715: Effects of miR-205 on cell migration and invasion of NPC cells. (A) Cell migration was determined by a wound healing assay. The promotion rates of cell migration were 56.8% in CNE2 cells (P=0.003), 108.6% in 6-10B cells (P=0.003) and 95.2% in 9-4E cells (P=0.004) after transfection with miR-205. (B) Cell invasion was determined by a Transwell assay. The number of invaded cells was increased in CNE2, 6-10B and 9-4E cells by 61.4% (P=0.000), 67.4% (P=0.008) and 86.6% (P=0.003), respectively, after transfection with miR-205. Cells are stained with crystal violet; magnification, ×10. Values are expressed as the mean ± standard deviation. *P<0.05; **P<0.001 vs. NC. miR, microRNA; NC, negative control; NPC, nasopharyngeal carcinoma.
Mentions: The wound-healing and invasion assays indicated that overexpression of miR-205 increased the rates of migration and invasion of CNE2, 6-10B and 9-4E cells compared with those of the negative control cells (Fig. 5A and B).

Bottom Line: The present study aimed to explore the potential role of miR‑205 in NPC.The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues.Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

ABSTRACT
Nasopharyngeal carcinoma (NPC) is a common type of cancer in southern China, miRNAs have been shown to be involved in the tumorigenesis of multiple cancer types. The present study aimed to explore the potential role of miR‑205 in NPC. Reverse transcription quantitative polymerase chain reaction was used to determine the expression levels of miR‑205 in 20 fresh NPC specimens and 20 normal nasopharyngeal tissues. The function of miR‑205 in the proliferation, migration, invasion and apoptosis of NPC‑derived cells was detected by MTT assay, colony formation assay, wound healing assay, Transwell assay and flow cytometry. Furthermore, a target gene of miR‑205 was identified using the luciferase reporter assay. The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues. Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed. Tumor protein p53-inducible nuclear protein 1 was identified as a target gene of miR‑205. Overall, the present study demonstrated that miR‑205 may function as an oncogene in NPC tumorigenesis.

Show MeSH
Related in: MedlinePlus