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MicroRNA‑205 promotes the tumorigenesis of nasopharyngeal carcinoma through targeting tumor protein p53-inducible nuclear protein 1.

Nie G, Duan H, Li X, Yu Z, Luo L, Lu R, Ji Z, Zhang W - Mol Med Rep (2015)

Bottom Line: The present study aimed to explore the potential role of miR‑205 in NPC.The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues.Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

ABSTRACT
Nasopharyngeal carcinoma (NPC) is a common type of cancer in southern China, miRNAs have been shown to be involved in the tumorigenesis of multiple cancer types. The present study aimed to explore the potential role of miR‑205 in NPC. Reverse transcription quantitative polymerase chain reaction was used to determine the expression levels of miR‑205 in 20 fresh NPC specimens and 20 normal nasopharyngeal tissues. The function of miR‑205 in the proliferation, migration, invasion and apoptosis of NPC‑derived cells was detected by MTT assay, colony formation assay, wound healing assay, Transwell assay and flow cytometry. Furthermore, a target gene of miR‑205 was identified using the luciferase reporter assay. The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues. Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed. Tumor protein p53-inducible nuclear protein 1 was identified as a target gene of miR‑205. Overall, the present study demonstrated that miR‑205 may function as an oncogene in NPC tumorigenesis.

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miR-205 promotes cell proliferation and colony formation of NPC cells. (A) The proliferation was determined using an MTT assay. The proliferation of CNE2 cells was increased by 12.5% (24 h), 18.6% (48 h) and 29.9% (72 h) (P=0.035), the proliferation of 6-10B cells was increased by 11.1% (24 h), 14.8% (48 h) and 14.3% (72 h) (P=0.001) and the proliferation of 9-4E cells was increased by 17.4% (24 h), 27.8% (48 h) and 38.6% (72 h) (P<0.001). (B) The clonogenic potential of the cells was determined using a colony formation assay. The promotion rates of colony formation were 75.4% in CNE2 cells, 271.7% in 6-10B cells and 66.9% in 9-4E cells. Values are expressed as the mean ± standard deviation. *P<0.05, **P<0.001 vs. NC. NPC, nasopharyngeal carcinoma; miR, microRNA; NC, negative control.
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f3-mmr-12-04-5715: miR-205 promotes cell proliferation and colony formation of NPC cells. (A) The proliferation was determined using an MTT assay. The proliferation of CNE2 cells was increased by 12.5% (24 h), 18.6% (48 h) and 29.9% (72 h) (P=0.035), the proliferation of 6-10B cells was increased by 11.1% (24 h), 14.8% (48 h) and 14.3% (72 h) (P=0.001) and the proliferation of 9-4E cells was increased by 17.4% (24 h), 27.8% (48 h) and 38.6% (72 h) (P<0.001). (B) The clonogenic potential of the cells was determined using a colony formation assay. The promotion rates of colony formation were 75.4% in CNE2 cells, 271.7% in 6-10B cells and 66.9% in 9-4E cells. Values are expressed as the mean ± standard deviation. *P<0.05, **P<0.001 vs. NC. NPC, nasopharyngeal carcinoma; miR, microRNA; NC, negative control.

Mentions: The MTT assay showed that the cell proliferation was significantly increased by overexpression of miR-205 in CNE2, 6-10B and 9-4E cells compared with that of the negative control cells. (Fig. 3A).


MicroRNA‑205 promotes the tumorigenesis of nasopharyngeal carcinoma through targeting tumor protein p53-inducible nuclear protein 1.

Nie G, Duan H, Li X, Yu Z, Luo L, Lu R, Ji Z, Zhang W - Mol Med Rep (2015)

miR-205 promotes cell proliferation and colony formation of NPC cells. (A) The proliferation was determined using an MTT assay. The proliferation of CNE2 cells was increased by 12.5% (24 h), 18.6% (48 h) and 29.9% (72 h) (P=0.035), the proliferation of 6-10B cells was increased by 11.1% (24 h), 14.8% (48 h) and 14.3% (72 h) (P=0.001) and the proliferation of 9-4E cells was increased by 17.4% (24 h), 27.8% (48 h) and 38.6% (72 h) (P<0.001). (B) The clonogenic potential of the cells was determined using a colony formation assay. The promotion rates of colony formation were 75.4% in CNE2 cells, 271.7% in 6-10B cells and 66.9% in 9-4E cells. Values are expressed as the mean ± standard deviation. *P<0.05, **P<0.001 vs. NC. NPC, nasopharyngeal carcinoma; miR, microRNA; NC, negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581759&req=5

f3-mmr-12-04-5715: miR-205 promotes cell proliferation and colony formation of NPC cells. (A) The proliferation was determined using an MTT assay. The proliferation of CNE2 cells was increased by 12.5% (24 h), 18.6% (48 h) and 29.9% (72 h) (P=0.035), the proliferation of 6-10B cells was increased by 11.1% (24 h), 14.8% (48 h) and 14.3% (72 h) (P=0.001) and the proliferation of 9-4E cells was increased by 17.4% (24 h), 27.8% (48 h) and 38.6% (72 h) (P<0.001). (B) The clonogenic potential of the cells was determined using a colony formation assay. The promotion rates of colony formation were 75.4% in CNE2 cells, 271.7% in 6-10B cells and 66.9% in 9-4E cells. Values are expressed as the mean ± standard deviation. *P<0.05, **P<0.001 vs. NC. NPC, nasopharyngeal carcinoma; miR, microRNA; NC, negative control.
Mentions: The MTT assay showed that the cell proliferation was significantly increased by overexpression of miR-205 in CNE2, 6-10B and 9-4E cells compared with that of the negative control cells. (Fig. 3A).

Bottom Line: The present study aimed to explore the potential role of miR‑205 in NPC.The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues.Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

ABSTRACT
Nasopharyngeal carcinoma (NPC) is a common type of cancer in southern China, miRNAs have been shown to be involved in the tumorigenesis of multiple cancer types. The present study aimed to explore the potential role of miR‑205 in NPC. Reverse transcription quantitative polymerase chain reaction was used to determine the expression levels of miR‑205 in 20 fresh NPC specimens and 20 normal nasopharyngeal tissues. The function of miR‑205 in the proliferation, migration, invasion and apoptosis of NPC‑derived cells was detected by MTT assay, colony formation assay, wound healing assay, Transwell assay and flow cytometry. Furthermore, a target gene of miR‑205 was identified using the luciferase reporter assay. The expression of miR‑205 was increased in NPC tissues compared with that in normal tissues. Overexpression of miR‑205 was found to promote the proliferation, migration and invasion of NPC‑derived cells, while apoptosis was suppressed. Tumor protein p53-inducible nuclear protein 1 was identified as a target gene of miR‑205. Overall, the present study demonstrated that miR‑205 may function as an oncogene in NPC tumorigenesis.

Show MeSH
Related in: MedlinePlus