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Essential oil of Artemisia vestita exhibits potent in vitro and in vivo antibacterial activity: Investigation of the effect of oil on biofilm formation, leakage of potassium ions and survival curve measurement.

Yang C, Hu DH, Feng Y - Mol Med Rep (2015)

Bottom Line: Survival curves indicated that the essential oil led to a reduction in the viability of different bacteria.The essential oil also induced significant leakage of potassium ions from S. pyogenes.In conclusion, the in vitro and in vivo results suggested that the essential oil of A. vestita and one of its major constituents, grandisol, can significantly inhibit the growth of different bacterial strains.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Zhongshan Hospital of Hubei Province, Wuhan, Hubei 430033, P.R. China.

ABSTRACT
The aim of the present study was to investigate the chemical composition of the essential oil of Artemisia vestita and to determine the antibacterial activity of the essential oil and its two major components, grandisol and 1,8‑cineole, against certain respiratory infection‑causing bacterial strains, in vitro and in vivo. The chemical composition of the essential oil was analyzed using gas chromatography‑mass spectrometry. A micro‑well dilution method was used to determine the minimum inhibition concentration (MIC) values of the essential oil and its major constituents. A model of Streptococcus pyogenes infection in mice was used to determine its in vivo activities. Lung and blood samples were obtained to assess bacterial cell counts. Toxicity evaluation of the essential oil and its components was completed by performing biochemical analysis of the serum, particularly monitoring aspartate transaminase, alanine transaminase, urea and creatinine. The essential oil exhibited potent antibacterial activity, whereas the two major constituents were less potent. The essential oil exhibited MIC values between 20 and 80 µg/ml, while the values of the two constituents were between 130 and 200 µg/ml. Scanning electron microscopy results demonstrated that the essential oil inhibited biofilm formation and altered its architecture. Survival curves indicated that the essential oil led to a reduction in the viability of different bacteria. The essential oil also induced significant leakage of potassium ions from S. pyogenes. The essential oil (100 µg/mouse) and grandisol (135 µg/mouse) significantly reduced the number of viable bacterial cells in the lungs (P<0.01). However, intake of 100 µg/mouse of essential oil or grandisol 135 µg/mouse once or twice each day for 9 days did not produce any toxic effects in the mice. In conclusion, the in vitro and in vivo results suggested that the essential oil of A. vestita and one of its major constituents, grandisol, can significantly inhibit the growth of different bacterial strains.

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Effects of treatment with 50 μg/ml Artemisia vestita essential oil on the efflux of potassium ions in cell suspensions containing S. pyogenes. S. pyogenes, Streptococcus pyogenes.
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f3-mmr-12-04-5762: Effects of treatment with 50 μg/ml Artemisia vestita essential oil on the efflux of potassium ions in cell suspensions containing S. pyogenes. S. pyogenes, Streptococcus pyogenes.

Mentions: A. vestita essential oil (50 μg/ml) induced potent leakage of K+ ions from S. pyogenes (Fig. 3). The results indicated that the leakage of K+ ions started immediately following the addition of A. vestita essential oil to the bacterial cultures of S. pyogenes. As Fig. 3 shows, following a time interval of 50 min, the extent of K+ ion leakage reached 100% of the total cellular free K+ free ions.


Essential oil of Artemisia vestita exhibits potent in vitro and in vivo antibacterial activity: Investigation of the effect of oil on biofilm formation, leakage of potassium ions and survival curve measurement.

Yang C, Hu DH, Feng Y - Mol Med Rep (2015)

Effects of treatment with 50 μg/ml Artemisia vestita essential oil on the efflux of potassium ions in cell suspensions containing S. pyogenes. S. pyogenes, Streptococcus pyogenes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581751&req=5

f3-mmr-12-04-5762: Effects of treatment with 50 μg/ml Artemisia vestita essential oil on the efflux of potassium ions in cell suspensions containing S. pyogenes. S. pyogenes, Streptococcus pyogenes.
Mentions: A. vestita essential oil (50 μg/ml) induced potent leakage of K+ ions from S. pyogenes (Fig. 3). The results indicated that the leakage of K+ ions started immediately following the addition of A. vestita essential oil to the bacterial cultures of S. pyogenes. As Fig. 3 shows, following a time interval of 50 min, the extent of K+ ion leakage reached 100% of the total cellular free K+ free ions.

Bottom Line: Survival curves indicated that the essential oil led to a reduction in the viability of different bacteria.The essential oil also induced significant leakage of potassium ions from S. pyogenes.In conclusion, the in vitro and in vivo results suggested that the essential oil of A. vestita and one of its major constituents, grandisol, can significantly inhibit the growth of different bacterial strains.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Zhongshan Hospital of Hubei Province, Wuhan, Hubei 430033, P.R. China.

ABSTRACT
The aim of the present study was to investigate the chemical composition of the essential oil of Artemisia vestita and to determine the antibacterial activity of the essential oil and its two major components, grandisol and 1,8‑cineole, against certain respiratory infection‑causing bacterial strains, in vitro and in vivo. The chemical composition of the essential oil was analyzed using gas chromatography‑mass spectrometry. A micro‑well dilution method was used to determine the minimum inhibition concentration (MIC) values of the essential oil and its major constituents. A model of Streptococcus pyogenes infection in mice was used to determine its in vivo activities. Lung and blood samples were obtained to assess bacterial cell counts. Toxicity evaluation of the essential oil and its components was completed by performing biochemical analysis of the serum, particularly monitoring aspartate transaminase, alanine transaminase, urea and creatinine. The essential oil exhibited potent antibacterial activity, whereas the two major constituents were less potent. The essential oil exhibited MIC values between 20 and 80 µg/ml, while the values of the two constituents were between 130 and 200 µg/ml. Scanning electron microscopy results demonstrated that the essential oil inhibited biofilm formation and altered its architecture. Survival curves indicated that the essential oil led to a reduction in the viability of different bacteria. The essential oil also induced significant leakage of potassium ions from S. pyogenes. The essential oil (100 µg/mouse) and grandisol (135 µg/mouse) significantly reduced the number of viable bacterial cells in the lungs (P<0.01). However, intake of 100 µg/mouse of essential oil or grandisol 135 µg/mouse once or twice each day for 9 days did not produce any toxic effects in the mice. In conclusion, the in vitro and in vivo results suggested that the essential oil of A. vestita and one of its major constituents, grandisol, can significantly inhibit the growth of different bacterial strains.

Show MeSH
Related in: MedlinePlus