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Polyphenolic composition of grape stem extracts affects antioxidant activity in endothelial and muscle cells.

Goutzourelas N, Stagos D, Spanidis Y, Liosi M, Apostolou A, Priftis A, Haroutounian S, Spandidos DA, Tsatsakis AM, Kouretas D - Mol Med Rep (2015)

Bottom Line: The results revealed that treatment of the EA.hy926 cells with Mandilaria extract significantly decreased the TBARS levels by 14.8% and the CARB levels by 25.9 %, while it increased the GSH levels by 15.8% compared to the controls.Treatment of the C2C12 cells with Mandilaria extract significantly decreased the TBARS levels by 47.3 %, the CARB levels by 39.0 % and the ROS levels by 21.8%, while it increased the GSH levels by 22.6% compared to the controls.In conclusion, to the best of our knowledgel, our results demonstrate for the first time that treatment with grape stem extracts at low concentrations improves the redox status of endothelial and muscle cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, 41221 Larissa, Greece.

ABSTRACT
The aim of the present study was the assessment of the antioxidant effects of polyphenolic extracts derived from the stems of three Greek grape varieties (Moshomayro, Mavrotragano and Mandilaria) in endothelial (EA.hy926) and muscle (C2C12) cells. We also investigated the effects of the polyphenolic composition on the antioxidant effects of the grape stem extracts. For this purpose, the endothelial and muscle cells were treated with low non-cytotoxic concentrations of the extracts for 24 h in order to assess the effects of the extracts on cellular redox status using oxidative stress biomarkers. The oxidative stress markers were thiobarbituric acid reactive substances (TBARS), protein carbonyl (CARB) levels, reactive oxygen species (ROS) levels and glutathione (GSH) levels. The results revealed that treatment of the EA.hy926 cells with Mandilaria extract significantly decreased the TBARS levels by 14.8% and the CARB levels by 25.9 %, while it increased the GSH levels by 15.8% compared to the controls. Moreover, treatment of the EA.hy926 cells with Mavrotragano extract significantly increased the GSH levels by 20.2%, while it significantly decreased the TBARS and CARB levels by 12.5% and 16.6%, respectively. Treatment of the C2C12 cells with Mandilaria extract significantly decreased the TBARS levels by 47.3 %, the CARB levels by 39.0 % and the ROS levels by 21.8%, while it increased the GSH levels by 22.6% compared to the controls. Moreover, treatment of the C2C12 cells with Mavrotragano significantly decreased the TBARS, CARB and ROS levels by 36.2%, 35.9% and 16.5%, respectively. In conclusion, to the best of our knowledgel, our results demonstrate for the first time that treatment with grape stem extracts at low concentrations improves the redox status of endothelial and muscle cells. Thus, grape stem extracts may be used for developing antioxidant food supplements or biofunctional foods. However, it was also found that the polyphenolic composition of grape stem extracts affects their antioxidant capacity. For example, the results suggested that trans-resveratrol, gallic acid, (+)-catechin, ferulic acid, caffeic acid, quercetin, coumaric acid and kaempferol may be essential for the antioxidant activity of grape stem extracts.

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(A) Percentage 2,2-diphenyl-1-picrylhydrazyl (DPPH●) radical scavenging activity, (B) percentage 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS●+) radical scavenging activity and (C) reducing power of grape stem extracts from Mavrotragano, Moshomavro and Mandilaria varieties. Values are presented as the means ± SEM.
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f1-mmr-12-04-5846: (A) Percentage 2,2-diphenyl-1-picrylhydrazyl (DPPH●) radical scavenging activity, (B) percentage 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS●+) radical scavenging activity and (C) reducing power of grape stem extracts from Mavrotragano, Moshomavro and Mandilaria varieties. Values are presented as the means ± SEM.

Mentions: All the extracts were examined for their free radical scavenging activity. The results revealed that all the extracts scavenged DPPH● and ABTS●+ radicals in a dose-dependent manner at low concentrations (Fig. 1A and B). In order to compare the radical scavenging efficiency of the extracts, the IC50 value indicating the concentration that caused the scavenging of 50% of DPPH● and ABTS●+ radicals was calculated; graphs were created to plot the percentage of radical scavenging capacity against the extract concentration. The IC50 values calcuated from the DPPH radical scavenging assay were in the following order: Mandilaria extract (2.8±0.2 µg polyphenols/ml) > Mavrotragano extract (3.6±0.17 µg polyphenols/ml) > Moshomavro extract (6.5±0.23 µg polyphenols/ml) (Table II). In the ABTS●+ radical scavenging assay, the order of potency of the extracts according to their IC50 values was: Mandilaria extract (0.42±0.08 µg polyphenols/ml) > Mavrotragano extract (0.71±0.10 µg polyphenols/ml) > Moshomavro extract (0.90±0.11 µg polyphenols/ml) (Table II).


Polyphenolic composition of grape stem extracts affects antioxidant activity in endothelial and muscle cells.

Goutzourelas N, Stagos D, Spanidis Y, Liosi M, Apostolou A, Priftis A, Haroutounian S, Spandidos DA, Tsatsakis AM, Kouretas D - Mol Med Rep (2015)

(A) Percentage 2,2-diphenyl-1-picrylhydrazyl (DPPH●) radical scavenging activity, (B) percentage 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS●+) radical scavenging activity and (C) reducing power of grape stem extracts from Mavrotragano, Moshomavro and Mandilaria varieties. Values are presented as the means ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581749&req=5

f1-mmr-12-04-5846: (A) Percentage 2,2-diphenyl-1-picrylhydrazyl (DPPH●) radical scavenging activity, (B) percentage 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS●+) radical scavenging activity and (C) reducing power of grape stem extracts from Mavrotragano, Moshomavro and Mandilaria varieties. Values are presented as the means ± SEM.
Mentions: All the extracts were examined for their free radical scavenging activity. The results revealed that all the extracts scavenged DPPH● and ABTS●+ radicals in a dose-dependent manner at low concentrations (Fig. 1A and B). In order to compare the radical scavenging efficiency of the extracts, the IC50 value indicating the concentration that caused the scavenging of 50% of DPPH● and ABTS●+ radicals was calculated; graphs were created to plot the percentage of radical scavenging capacity against the extract concentration. The IC50 values calcuated from the DPPH radical scavenging assay were in the following order: Mandilaria extract (2.8±0.2 µg polyphenols/ml) > Mavrotragano extract (3.6±0.17 µg polyphenols/ml) > Moshomavro extract (6.5±0.23 µg polyphenols/ml) (Table II). In the ABTS●+ radical scavenging assay, the order of potency of the extracts according to their IC50 values was: Mandilaria extract (0.42±0.08 µg polyphenols/ml) > Mavrotragano extract (0.71±0.10 µg polyphenols/ml) > Moshomavro extract (0.90±0.11 µg polyphenols/ml) (Table II).

Bottom Line: The results revealed that treatment of the EA.hy926 cells with Mandilaria extract significantly decreased the TBARS levels by 14.8% and the CARB levels by 25.9 %, while it increased the GSH levels by 15.8% compared to the controls.Treatment of the C2C12 cells with Mandilaria extract significantly decreased the TBARS levels by 47.3 %, the CARB levels by 39.0 % and the ROS levels by 21.8%, while it increased the GSH levels by 22.6% compared to the controls.In conclusion, to the best of our knowledgel, our results demonstrate for the first time that treatment with grape stem extracts at low concentrations improves the redox status of endothelial and muscle cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, 41221 Larissa, Greece.

ABSTRACT
The aim of the present study was the assessment of the antioxidant effects of polyphenolic extracts derived from the stems of three Greek grape varieties (Moshomayro, Mavrotragano and Mandilaria) in endothelial (EA.hy926) and muscle (C2C12) cells. We also investigated the effects of the polyphenolic composition on the antioxidant effects of the grape stem extracts. For this purpose, the endothelial and muscle cells were treated with low non-cytotoxic concentrations of the extracts for 24 h in order to assess the effects of the extracts on cellular redox status using oxidative stress biomarkers. The oxidative stress markers were thiobarbituric acid reactive substances (TBARS), protein carbonyl (CARB) levels, reactive oxygen species (ROS) levels and glutathione (GSH) levels. The results revealed that treatment of the EA.hy926 cells with Mandilaria extract significantly decreased the TBARS levels by 14.8% and the CARB levels by 25.9 %, while it increased the GSH levels by 15.8% compared to the controls. Moreover, treatment of the EA.hy926 cells with Mavrotragano extract significantly increased the GSH levels by 20.2%, while it significantly decreased the TBARS and CARB levels by 12.5% and 16.6%, respectively. Treatment of the C2C12 cells with Mandilaria extract significantly decreased the TBARS levels by 47.3 %, the CARB levels by 39.0 % and the ROS levels by 21.8%, while it increased the GSH levels by 22.6% compared to the controls. Moreover, treatment of the C2C12 cells with Mavrotragano significantly decreased the TBARS, CARB and ROS levels by 36.2%, 35.9% and 16.5%, respectively. In conclusion, to the best of our knowledgel, our results demonstrate for the first time that treatment with grape stem extracts at low concentrations improves the redox status of endothelial and muscle cells. Thus, grape stem extracts may be used for developing antioxidant food supplements or biofunctional foods. However, it was also found that the polyphenolic composition of grape stem extracts affects their antioxidant capacity. For example, the results suggested that trans-resveratrol, gallic acid, (+)-catechin, ferulic acid, caffeic acid, quercetin, coumaric acid and kaempferol may be essential for the antioxidant activity of grape stem extracts.

Show MeSH
Related in: MedlinePlus