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An in vitro investigation into the role of bone marrow‑derived mesenchymal stem cells in the control of disc degeneration.

Hu J, Deng G, Tian Y, Pu Y, Cao P, Yuan W - Mol Med Rep (2015)

Bottom Line: The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs.In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study.The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changzheng Hospital, Shanghai 200023, P.R. China.

ABSTRACT
Excessive apoptosis and high expression levels of interleukin‑1β (IL‑1β) in disc cells have been reported to serve important roles in intervertebral disc degeneration (IVDD). Previous studies investigating mesenchymal stem cells (MSCs) have indicated potential for their use in the treatment of IVDD. However, the therapeutic potential and anti‑apoptotic ability of MSCs remains to be fully elucidated. The present study aimed to establish an in vitro model for bone marrow‑derived MSC (BMSC) therapy by investigating the anti‑apoptotic effects, in addition to the migration of BMSCs to nucleus pulposus (NP) cells stimulated by IL‑1β. A co-culture system of BMSCs and NP cells was founded. Following inflammatory stimulation, the NP cells exhibited increased indexes for inflammation‑induced degeneration. The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs. Compared with the indirect co-culture group, the direct co-culture group exhibited an improved capacity for anti-apoptosis. In addition, IL‑1β‑stimulated NP cells attracted and mediated the migration of BMSCs. Mitochondrial transfer from BMSCs to NP cells by tunneling nanotubes was also observed. In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study. The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

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Mitochondrial transfer measured by confocal microscopy. Phase-contrast images were captured in three fluorescence channels. (A) DAPI, (B) GFP (fluorescein isothiocyanate), (C) MitoTracker® Deep Red and (D) merged image. The arrows indicate thin TnT-like structures. Pre-lableling MitoTracker® (red) mitochondria were observed transferring from BMSCs to stimulated nucleus pulposus cells through TnT-like structures at 24 h of co-culture. Scale bars, 50 µm. TnT, tunneling nanotubes; GFP, green fluorescent protein; BMSCs, bone marrow-derived mesenchymal stem cells.
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f6-mmr-12-04-5701: Mitochondrial transfer measured by confocal microscopy. Phase-contrast images were captured in three fluorescence channels. (A) DAPI, (B) GFP (fluorescein isothiocyanate), (C) MitoTracker® Deep Red and (D) merged image. The arrows indicate thin TnT-like structures. Pre-lableling MitoTracker® (red) mitochondria were observed transferring from BMSCs to stimulated nucleus pulposus cells through TnT-like structures at 24 h of co-culture. Scale bars, 50 µm. TnT, tunneling nanotubes; GFP, green fluorescent protein; BMSCs, bone marrow-derived mesenchymal stem cells.

Mentions: The arrows in Fig. 6 indicate that TnT-like structures were observed between BMSCs and NP cells by confocal microscopy. Mitochondria were clearly observed in the red fluorescence channel, transferring from relabeled GFP BMSCs to stimulated NP cells via TnTs (Fig. 6).


An in vitro investigation into the role of bone marrow‑derived mesenchymal stem cells in the control of disc degeneration.

Hu J, Deng G, Tian Y, Pu Y, Cao P, Yuan W - Mol Med Rep (2015)

Mitochondrial transfer measured by confocal microscopy. Phase-contrast images were captured in three fluorescence channels. (A) DAPI, (B) GFP (fluorescein isothiocyanate), (C) MitoTracker® Deep Red and (D) merged image. The arrows indicate thin TnT-like structures. Pre-lableling MitoTracker® (red) mitochondria were observed transferring from BMSCs to stimulated nucleus pulposus cells through TnT-like structures at 24 h of co-culture. Scale bars, 50 µm. TnT, tunneling nanotubes; GFP, green fluorescent protein; BMSCs, bone marrow-derived mesenchymal stem cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581747&req=5

f6-mmr-12-04-5701: Mitochondrial transfer measured by confocal microscopy. Phase-contrast images were captured in three fluorescence channels. (A) DAPI, (B) GFP (fluorescein isothiocyanate), (C) MitoTracker® Deep Red and (D) merged image. The arrows indicate thin TnT-like structures. Pre-lableling MitoTracker® (red) mitochondria were observed transferring from BMSCs to stimulated nucleus pulposus cells through TnT-like structures at 24 h of co-culture. Scale bars, 50 µm. TnT, tunneling nanotubes; GFP, green fluorescent protein; BMSCs, bone marrow-derived mesenchymal stem cells.
Mentions: The arrows in Fig. 6 indicate that TnT-like structures were observed between BMSCs and NP cells by confocal microscopy. Mitochondria were clearly observed in the red fluorescence channel, transferring from relabeled GFP BMSCs to stimulated NP cells via TnTs (Fig. 6).

Bottom Line: The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs.In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study.The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changzheng Hospital, Shanghai 200023, P.R. China.

ABSTRACT
Excessive apoptosis and high expression levels of interleukin‑1β (IL‑1β) in disc cells have been reported to serve important roles in intervertebral disc degeneration (IVDD). Previous studies investigating mesenchymal stem cells (MSCs) have indicated potential for their use in the treatment of IVDD. However, the therapeutic potential and anti‑apoptotic ability of MSCs remains to be fully elucidated. The present study aimed to establish an in vitro model for bone marrow‑derived MSC (BMSC) therapy by investigating the anti‑apoptotic effects, in addition to the migration of BMSCs to nucleus pulposus (NP) cells stimulated by IL‑1β. A co-culture system of BMSCs and NP cells was founded. Following inflammatory stimulation, the NP cells exhibited increased indexes for inflammation‑induced degeneration. The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs. Compared with the indirect co-culture group, the direct co-culture group exhibited an improved capacity for anti-apoptosis. In addition, IL‑1β‑stimulated NP cells attracted and mediated the migration of BMSCs. Mitochondrial transfer from BMSCs to NP cells by tunneling nanotubes was also observed. In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study. The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

Show MeSH
Related in: MedlinePlus