Limits...
An in vitro investigation into the role of bone marrow‑derived mesenchymal stem cells in the control of disc degeneration.

Hu J, Deng G, Tian Y, Pu Y, Cao P, Yuan W - Mol Med Rep (2015)

Bottom Line: The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs.In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study.The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changzheng Hospital, Shanghai 200023, P.R. China.

ABSTRACT
Excessive apoptosis and high expression levels of interleukin‑1β (IL‑1β) in disc cells have been reported to serve important roles in intervertebral disc degeneration (IVDD). Previous studies investigating mesenchymal stem cells (MSCs) have indicated potential for their use in the treatment of IVDD. However, the therapeutic potential and anti‑apoptotic ability of MSCs remains to be fully elucidated. The present study aimed to establish an in vitro model for bone marrow‑derived MSC (BMSC) therapy by investigating the anti‑apoptotic effects, in addition to the migration of BMSCs to nucleus pulposus (NP) cells stimulated by IL‑1β. A co-culture system of BMSCs and NP cells was founded. Following inflammatory stimulation, the NP cells exhibited increased indexes for inflammation‑induced degeneration. The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs. Compared with the indirect co-culture group, the direct co-culture group exhibited an improved capacity for anti-apoptosis. In addition, IL‑1β‑stimulated NP cells attracted and mediated the migration of BMSCs. Mitochondrial transfer from BMSCs to NP cells by tunneling nanotubes was also observed. In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study. The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

Show MeSH

Related in: MedlinePlus

Apoptosis rate detection by TUNEL-staining and caspase-3 ELISA kit. (A) The apoptotic response of the nucleus pulposus cells without simulation was low. At 24 h following stimulation with IL-1β (20 ng/ml), the level of apoptosis was significantly increased and the indirect co-culture significantly reversed this increase. (B) Using the caspase-3 ELISA kit, comparable results were obtained with 20 and 50 ng/ml IL-1β (*P<0.05). IL-1β; interleukin-1β.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581747&req=5

f3-mmr-12-04-5701: Apoptosis rate detection by TUNEL-staining and caspase-3 ELISA kit. (A) The apoptotic response of the nucleus pulposus cells without simulation was low. At 24 h following stimulation with IL-1β (20 ng/ml), the level of apoptosis was significantly increased and the indirect co-culture significantly reversed this increase. (B) Using the caspase-3 ELISA kit, comparable results were obtained with 20 and 50 ng/ml IL-1β (*P<0.05). IL-1β; interleukin-1β.

Mentions: TUNEL staining allows for the detection and quantification of apoptosis at the cellular level. The number of TUNEL-positive cells increased with inflammatory factors (20 ng/ml), whereas the number of TUNEL-positive cells was reduced in the co-culture group (Fig. 3A).


An in vitro investigation into the role of bone marrow‑derived mesenchymal stem cells in the control of disc degeneration.

Hu J, Deng G, Tian Y, Pu Y, Cao P, Yuan W - Mol Med Rep (2015)

Apoptosis rate detection by TUNEL-staining and caspase-3 ELISA kit. (A) The apoptotic response of the nucleus pulposus cells without simulation was low. At 24 h following stimulation with IL-1β (20 ng/ml), the level of apoptosis was significantly increased and the indirect co-culture significantly reversed this increase. (B) Using the caspase-3 ELISA kit, comparable results were obtained with 20 and 50 ng/ml IL-1β (*P<0.05). IL-1β; interleukin-1β.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581747&req=5

f3-mmr-12-04-5701: Apoptosis rate detection by TUNEL-staining and caspase-3 ELISA kit. (A) The apoptotic response of the nucleus pulposus cells without simulation was low. At 24 h following stimulation with IL-1β (20 ng/ml), the level of apoptosis was significantly increased and the indirect co-culture significantly reversed this increase. (B) Using the caspase-3 ELISA kit, comparable results were obtained with 20 and 50 ng/ml IL-1β (*P<0.05). IL-1β; interleukin-1β.
Mentions: TUNEL staining allows for the detection and quantification of apoptosis at the cellular level. The number of TUNEL-positive cells increased with inflammatory factors (20 ng/ml), whereas the number of TUNEL-positive cells was reduced in the co-culture group (Fig. 3A).

Bottom Line: The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs.In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study.The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changzheng Hospital, Shanghai 200023, P.R. China.

ABSTRACT
Excessive apoptosis and high expression levels of interleukin‑1β (IL‑1β) in disc cells have been reported to serve important roles in intervertebral disc degeneration (IVDD). Previous studies investigating mesenchymal stem cells (MSCs) have indicated potential for their use in the treatment of IVDD. However, the therapeutic potential and anti‑apoptotic ability of MSCs remains to be fully elucidated. The present study aimed to establish an in vitro model for bone marrow‑derived MSC (BMSC) therapy by investigating the anti‑apoptotic effects, in addition to the migration of BMSCs to nucleus pulposus (NP) cells stimulated by IL‑1β. A co-culture system of BMSCs and NP cells was founded. Following inflammatory stimulation, the NP cells exhibited increased indexes for inflammation‑induced degeneration. The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs. Compared with the indirect co-culture group, the direct co-culture group exhibited an improved capacity for anti-apoptosis. In addition, IL‑1β‑stimulated NP cells attracted and mediated the migration of BMSCs. Mitochondrial transfer from BMSCs to NP cells by tunneling nanotubes was also observed. In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study. The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

Show MeSH
Related in: MedlinePlus