Limits...
An in vitro investigation into the role of bone marrow‑derived mesenchymal stem cells in the control of disc degeneration.

Hu J, Deng G, Tian Y, Pu Y, Cao P, Yuan W - Mol Med Rep (2015)

Bottom Line: The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs.In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study.The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changzheng Hospital, Shanghai 200023, P.R. China.

ABSTRACT
Excessive apoptosis and high expression levels of interleukin‑1β (IL‑1β) in disc cells have been reported to serve important roles in intervertebral disc degeneration (IVDD). Previous studies investigating mesenchymal stem cells (MSCs) have indicated potential for their use in the treatment of IVDD. However, the therapeutic potential and anti‑apoptotic ability of MSCs remains to be fully elucidated. The present study aimed to establish an in vitro model for bone marrow‑derived MSC (BMSC) therapy by investigating the anti‑apoptotic effects, in addition to the migration of BMSCs to nucleus pulposus (NP) cells stimulated by IL‑1β. A co-culture system of BMSCs and NP cells was founded. Following inflammatory stimulation, the NP cells exhibited increased indexes for inflammation‑induced degeneration. The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs. Compared with the indirect co-culture group, the direct co-culture group exhibited an improved capacity for anti-apoptosis. In addition, IL‑1β‑stimulated NP cells attracted and mediated the migration of BMSCs. Mitochondrial transfer from BMSCs to NP cells by tunneling nanotubes was also observed. In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study. The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

Show MeSH

Related in: MedlinePlus

Reverse transcription-quantitative polymerase chain reaction analysis of the expression of (A) ADAMTS-4, (B) ADAMTS-5, (C) caspase-3, (D) MMP-13 and (E) TIMP-1. Significant reductions were observed following IL-1β (20 ng/ml) stimulation, and there was a clear increase in expression subsequent to bone marrow-derived mesenchymal stem cell indirect co-culture. The data are presented as the mean ± standard error of three independent experiments (*P<0.05 and **P<0.01). ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; IL-1β; interleukin-1β.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581747&req=5

f2-mmr-12-04-5701: Reverse transcription-quantitative polymerase chain reaction analysis of the expression of (A) ADAMTS-4, (B) ADAMTS-5, (C) caspase-3, (D) MMP-13 and (E) TIMP-1. Significant reductions were observed following IL-1β (20 ng/ml) stimulation, and there was a clear increase in expression subsequent to bone marrow-derived mesenchymal stem cell indirect co-culture. The data are presented as the mean ± standard error of three independent experiments (*P<0.05 and **P<0.01). ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; IL-1β; interleukin-1β.

Mentions: Compared with the unstimulated NP cells, the NP cells in the no co-culture groups exhibited markedly higher expression levels of ADAMTS-4, ADAMTS-5, caspase-3 and MMP-13 following inflammatory stimulation (Fig. 2A–D), whereas the expression of TIMP-1 was reduced (Fig. 2E). Compared with the no co-culture group, various indexes were significantly reduced in the co-culture group. In addition, the expression of TIMP-1 was increased.


An in vitro investigation into the role of bone marrow‑derived mesenchymal stem cells in the control of disc degeneration.

Hu J, Deng G, Tian Y, Pu Y, Cao P, Yuan W - Mol Med Rep (2015)

Reverse transcription-quantitative polymerase chain reaction analysis of the expression of (A) ADAMTS-4, (B) ADAMTS-5, (C) caspase-3, (D) MMP-13 and (E) TIMP-1. Significant reductions were observed following IL-1β (20 ng/ml) stimulation, and there was a clear increase in expression subsequent to bone marrow-derived mesenchymal stem cell indirect co-culture. The data are presented as the mean ± standard error of three independent experiments (*P<0.05 and **P<0.01). ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; IL-1β; interleukin-1β.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581747&req=5

f2-mmr-12-04-5701: Reverse transcription-quantitative polymerase chain reaction analysis of the expression of (A) ADAMTS-4, (B) ADAMTS-5, (C) caspase-3, (D) MMP-13 and (E) TIMP-1. Significant reductions were observed following IL-1β (20 ng/ml) stimulation, and there was a clear increase in expression subsequent to bone marrow-derived mesenchymal stem cell indirect co-culture. The data are presented as the mean ± standard error of three independent experiments (*P<0.05 and **P<0.01). ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; IL-1β; interleukin-1β.
Mentions: Compared with the unstimulated NP cells, the NP cells in the no co-culture groups exhibited markedly higher expression levels of ADAMTS-4, ADAMTS-5, caspase-3 and MMP-13 following inflammatory stimulation (Fig. 2A–D), whereas the expression of TIMP-1 was reduced (Fig. 2E). Compared with the no co-culture group, various indexes were significantly reduced in the co-culture group. In addition, the expression of TIMP-1 was increased.

Bottom Line: The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs.In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study.The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changzheng Hospital, Shanghai 200023, P.R. China.

ABSTRACT
Excessive apoptosis and high expression levels of interleukin‑1β (IL‑1β) in disc cells have been reported to serve important roles in intervertebral disc degeneration (IVDD). Previous studies investigating mesenchymal stem cells (MSCs) have indicated potential for their use in the treatment of IVDD. However, the therapeutic potential and anti‑apoptotic ability of MSCs remains to be fully elucidated. The present study aimed to establish an in vitro model for bone marrow‑derived MSC (BMSC) therapy by investigating the anti‑apoptotic effects, in addition to the migration of BMSCs to nucleus pulposus (NP) cells stimulated by IL‑1β. A co-culture system of BMSCs and NP cells was founded. Following inflammatory stimulation, the NP cells exhibited increased indexes for inflammation‑induced degeneration. The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs. Compared with the indirect co-culture group, the direct co-culture group exhibited an improved capacity for anti-apoptosis. In addition, IL‑1β‑stimulated NP cells attracted and mediated the migration of BMSCs. Mitochondrial transfer from BMSCs to NP cells by tunneling nanotubes was also observed. In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study. The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

Show MeSH
Related in: MedlinePlus