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An in vitro investigation into the role of bone marrow‑derived mesenchymal stem cells in the control of disc degeneration.

Hu J, Deng G, Tian Y, Pu Y, Cao P, Yuan W - Mol Med Rep (2015)

Bottom Line: The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs.In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study.The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changzheng Hospital, Shanghai 200023, P.R. China.

ABSTRACT
Excessive apoptosis and high expression levels of interleukin‑1β (IL‑1β) in disc cells have been reported to serve important roles in intervertebral disc degeneration (IVDD). Previous studies investigating mesenchymal stem cells (MSCs) have indicated potential for their use in the treatment of IVDD. However, the therapeutic potential and anti‑apoptotic ability of MSCs remains to be fully elucidated. The present study aimed to establish an in vitro model for bone marrow‑derived MSC (BMSC) therapy by investigating the anti‑apoptotic effects, in addition to the migration of BMSCs to nucleus pulposus (NP) cells stimulated by IL‑1β. A co-culture system of BMSCs and NP cells was founded. Following inflammatory stimulation, the NP cells exhibited increased indexes for inflammation‑induced degeneration. The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs. Compared with the indirect co-culture group, the direct co-culture group exhibited an improved capacity for anti-apoptosis. In addition, IL‑1β‑stimulated NP cells attracted and mediated the migration of BMSCs. Mitochondrial transfer from BMSCs to NP cells by tunneling nanotubes was also observed. In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study. The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

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Identification of isolated BMSCs. Flow cytometric analysis of (A) CD90, (B) CD45, (C) CD29 and (D) CD31. BMSCs (>85%) were positive for CD29 and CD90, whereas <0.1% of the BMSCs were positive for CD31 and CD45. Following 2 week induced differentiation, (E) osteogenic differentiation of the cells was examined by Alizarin red staining and (F) adipogenic differentiation was assessed by Oil Red O staining. BMSCs, bone marrow-derived mesenchymal stem cells; CD90, cluster of differentiation 90.
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f1-mmr-12-04-5701: Identification of isolated BMSCs. Flow cytometric analysis of (A) CD90, (B) CD45, (C) CD29 and (D) CD31. BMSCs (>85%) were positive for CD29 and CD90, whereas <0.1% of the BMSCs were positive for CD31 and CD45. Following 2 week induced differentiation, (E) osteogenic differentiation of the cells was examined by Alizarin red staining and (F) adipogenic differentiation was assessed by Oil Red O staining. BMSCs, bone marrow-derived mesenchymal stem cells; CD90, cluster of differentiation 90.

Mentions: Flow cytometric analysis was used to detect CD90 (Fig. 1A), CD45 (Fig. 1B), CD29 (Fig. 1C) and CD90 (Fig. 1D) expression. BMSCs (>85%) were positive for CD31 and CD45, whereas <0.1% of BMSCs were positive for CD31 and CD45. Following 2 weeks of induced differentiation, osteogenic differentiation was examined by Alizarin red staining (Fig. 1E), and adipogenic differentiation was assessed by Oil Red O staining (Fig. 1F). The results suggest that cells cultured in this manner can be induced to differntiate into both osteogenic and adipogenic cells.


An in vitro investigation into the role of bone marrow‑derived mesenchymal stem cells in the control of disc degeneration.

Hu J, Deng G, Tian Y, Pu Y, Cao P, Yuan W - Mol Med Rep (2015)

Identification of isolated BMSCs. Flow cytometric analysis of (A) CD90, (B) CD45, (C) CD29 and (D) CD31. BMSCs (>85%) were positive for CD29 and CD90, whereas <0.1% of the BMSCs were positive for CD31 and CD45. Following 2 week induced differentiation, (E) osteogenic differentiation of the cells was examined by Alizarin red staining and (F) adipogenic differentiation was assessed by Oil Red O staining. BMSCs, bone marrow-derived mesenchymal stem cells; CD90, cluster of differentiation 90.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581747&req=5

f1-mmr-12-04-5701: Identification of isolated BMSCs. Flow cytometric analysis of (A) CD90, (B) CD45, (C) CD29 and (D) CD31. BMSCs (>85%) were positive for CD29 and CD90, whereas <0.1% of the BMSCs were positive for CD31 and CD45. Following 2 week induced differentiation, (E) osteogenic differentiation of the cells was examined by Alizarin red staining and (F) adipogenic differentiation was assessed by Oil Red O staining. BMSCs, bone marrow-derived mesenchymal stem cells; CD90, cluster of differentiation 90.
Mentions: Flow cytometric analysis was used to detect CD90 (Fig. 1A), CD45 (Fig. 1B), CD29 (Fig. 1C) and CD90 (Fig. 1D) expression. BMSCs (>85%) were positive for CD31 and CD45, whereas <0.1% of BMSCs were positive for CD31 and CD45. Following 2 weeks of induced differentiation, osteogenic differentiation was examined by Alizarin red staining (Fig. 1E), and adipogenic differentiation was assessed by Oil Red O staining (Fig. 1F). The results suggest that cells cultured in this manner can be induced to differntiate into both osteogenic and adipogenic cells.

Bottom Line: The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs.In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study.The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Changzheng Hospital, Shanghai 200023, P.R. China.

ABSTRACT
Excessive apoptosis and high expression levels of interleukin‑1β (IL‑1β) in disc cells have been reported to serve important roles in intervertebral disc degeneration (IVDD). Previous studies investigating mesenchymal stem cells (MSCs) have indicated potential for their use in the treatment of IVDD. However, the therapeutic potential and anti‑apoptotic ability of MSCs remains to be fully elucidated. The present study aimed to establish an in vitro model for bone marrow‑derived MSC (BMSC) therapy by investigating the anti‑apoptotic effects, in addition to the migration of BMSCs to nucleus pulposus (NP) cells stimulated by IL‑1β. A co-culture system of BMSCs and NP cells was founded. Following inflammatory stimulation, the NP cells exhibited increased indexes for inflammation‑induced degeneration. The degenerative and apoptotic indexes were significantly reduced when NP cells were co‑cultured with BMSCs. Compared with the indirect co-culture group, the direct co-culture group exhibited an improved capacity for anti-apoptosis. In addition, IL‑1β‑stimulated NP cells attracted and mediated the migration of BMSCs. Mitochondrial transfer from BMSCs to NP cells by tunneling nanotubes was also observed. In conclusion, the anti‑apoptosis and the migration, in addition to mitochondrial transfer associated with BMSC treatments in IVDD, were investigated in vitro in the present study. The interaction between stimulated NP cells and BMSCs is likely involved in to simulating the in vivo process of stem cell‑mediated repair.

Show MeSH
Related in: MedlinePlus