Limits...
Aberrant Wnt/β-catenin signaling and elevated expression of stem cell proteins are associated with osteosarcoma side population cells of high tumorigenicity.

Yi XJ, Zhao YH, Qiao LX, Jin CL, Tian J, Li QS - Mol Med Rep (2015)

Bottom Line: The results of subsequent western blot and reverse transcription‑quantitative polymerase chain reaction analyses revealed that the protein levels of β‑catenin and cyclin D1 were markedly upregulated in the fluorescence‑activated cell sorted osteosarcoma SP cells.In addition, the elevated expression levels of stem cell proteins, including CD133, nestin Oct‑4, Sox‑2 and Nanog were significantly higher in the SP cells, which contributed to self‑renewal and enhanced the proliferation rate of the SP cells.Taken together, these data suggested that the identification of novel anticancer drugs, which suppress the Wnt/β‑catenin signaling and its downstream pathway may assist in eradicating osteosarcoma stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Traumatology, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China.

ABSTRACT
According to the cancer stem cell theory, the presence of a small sub‑population of cancer cells, termed cancer stem cells (CSCs), have a significant implication on cancer treatment and are responsible for tumor recurrence. Previous studies have reported that alterations in the Wnt/β‑catenin signaling are crucial in the maintenance of CSCs. In the present study, the characteristic features and activation of Wnt/β‑catenin signaling in CSCs from osteosarcoma, an aggressive human bone tumor, were investigated. In total, ~2.1% of the cancer stem‑like side population (SP) cells were identified in the osteosarcoma samples. The results of subsequent western blot and reverse transcription‑quantitative polymerase chain reaction analyses revealed that the protein levels of β‑catenin and cyclin D1 were markedly upregulated in the fluorescence‑activated cell sorted osteosarcoma SP cells. In addition, the elevated expression levels of stem cell proteins, including CD133, nestin Oct‑4, Sox‑2 and Nanog were significantly higher in the SP cells, which contributed to self‑renewal and enhanced the proliferation rate of the SP cells. Furthermore, the SP cells were found to be highly invasive and able to form tumors in vivo. Taken together, these data suggested that the identification of novel anticancer drugs, which suppress the Wnt/β‑catenin signaling and its downstream pathway may assist in eradicating osteosarcoma stem cells.

Show MeSH

Related in: MedlinePlus

Tumorigenic potential of osteosarcoma SP cells. Gross tumor images of the tumors derived after 27 days of subcutaneously injected SP and non-SP cells into NOD/SCID mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581745&req=5

f5-mmr-12-04-5042: Tumorigenic potential of osteosarcoma SP cells. Gross tumor images of the tumors derived after 27 days of subcutaneously injected SP and non-SP cells into NOD/SCID mice.

Mentions: The SP cells were able to regenerate tumors even following transplantation at the lowest cell density (5,000 cells), however, the non-SP cells were unable to repopulate the tumor cells at this cell concentration (data not shown). As shown in Fig. 5, injection of the SP cells into the NOD/SCID mice formed tumor spheres in vivo and these tumors grew significantly faster than the tumors formed from non-SP cells. Therefore, the osteosarcoma SP cells exhibited multi-drug resistance, enhanced survival rate and increased tumorigenicity.


Aberrant Wnt/β-catenin signaling and elevated expression of stem cell proteins are associated with osteosarcoma side population cells of high tumorigenicity.

Yi XJ, Zhao YH, Qiao LX, Jin CL, Tian J, Li QS - Mol Med Rep (2015)

Tumorigenic potential of osteosarcoma SP cells. Gross tumor images of the tumors derived after 27 days of subcutaneously injected SP and non-SP cells into NOD/SCID mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581745&req=5

f5-mmr-12-04-5042: Tumorigenic potential of osteosarcoma SP cells. Gross tumor images of the tumors derived after 27 days of subcutaneously injected SP and non-SP cells into NOD/SCID mice.
Mentions: The SP cells were able to regenerate tumors even following transplantation at the lowest cell density (5,000 cells), however, the non-SP cells were unable to repopulate the tumor cells at this cell concentration (data not shown). As shown in Fig. 5, injection of the SP cells into the NOD/SCID mice formed tumor spheres in vivo and these tumors grew significantly faster than the tumors formed from non-SP cells. Therefore, the osteosarcoma SP cells exhibited multi-drug resistance, enhanced survival rate and increased tumorigenicity.

Bottom Line: The results of subsequent western blot and reverse transcription‑quantitative polymerase chain reaction analyses revealed that the protein levels of β‑catenin and cyclin D1 were markedly upregulated in the fluorescence‑activated cell sorted osteosarcoma SP cells.In addition, the elevated expression levels of stem cell proteins, including CD133, nestin Oct‑4, Sox‑2 and Nanog were significantly higher in the SP cells, which contributed to self‑renewal and enhanced the proliferation rate of the SP cells.Taken together, these data suggested that the identification of novel anticancer drugs, which suppress the Wnt/β‑catenin signaling and its downstream pathway may assist in eradicating osteosarcoma stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Traumatology, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China.

ABSTRACT
According to the cancer stem cell theory, the presence of a small sub‑population of cancer cells, termed cancer stem cells (CSCs), have a significant implication on cancer treatment and are responsible for tumor recurrence. Previous studies have reported that alterations in the Wnt/β‑catenin signaling are crucial in the maintenance of CSCs. In the present study, the characteristic features and activation of Wnt/β‑catenin signaling in CSCs from osteosarcoma, an aggressive human bone tumor, were investigated. In total, ~2.1% of the cancer stem‑like side population (SP) cells were identified in the osteosarcoma samples. The results of subsequent western blot and reverse transcription‑quantitative polymerase chain reaction analyses revealed that the protein levels of β‑catenin and cyclin D1 were markedly upregulated in the fluorescence‑activated cell sorted osteosarcoma SP cells. In addition, the elevated expression levels of stem cell proteins, including CD133, nestin Oct‑4, Sox‑2 and Nanog were significantly higher in the SP cells, which contributed to self‑renewal and enhanced the proliferation rate of the SP cells. Furthermore, the SP cells were found to be highly invasive and able to form tumors in vivo. Taken together, these data suggested that the identification of novel anticancer drugs, which suppress the Wnt/β‑catenin signaling and its downstream pathway may assist in eradicating osteosarcoma stem cells.

Show MeSH
Related in: MedlinePlus