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CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae.

Watve SS, Thomas J, Hammer BK - PLoS ONE (2015)

Bottom Line: Through high-throughput RNA sequencing (RNA-seq), we show that CytR positively regulates the majority of competence genes, the three type VI secretion operons, and the four known or predicted chitinases.We used transcriptional reporters and phenotypic analysis to determine the individual contributions of quorum sensing, which is controlled by the transcription factors HapR and QstR; chitin utilization that is mediated by TfoX; and pyrimidine starvation that is orchestrated by CytR, toward each of these processes.We find that in V. cholerae, CytR is a global regulator of multiple behaviors affecting fitness and adaptability in the environment.

View Article: PubMed Central - PubMed

Affiliation: School of Biology, Georgia Institute of Technology, Atlanta, Georgia, United States of America.

ABSTRACT
The facultative pathogen Vibrio cholerae transitions between its human host and aquatic reservoirs where it colonizes chitinous surfaces. Growth on chitin induces expression of chitin utilization genes, genes involved in DNA uptake by natural transformation, and a type VI secretion system that allows contact-dependent killing of neighboring bacteria. We have previously shown that the transcription factor CytR, thought to primarily regulate the pyrimidine nucleoside scavenging response, is required for natural competence in V. cholerae. Through high-throughput RNA sequencing (RNA-seq), we show that CytR positively regulates the majority of competence genes, the three type VI secretion operons, and the four known or predicted chitinases. We used transcriptional reporters and phenotypic analysis to determine the individual contributions of quorum sensing, which is controlled by the transcription factors HapR and QstR; chitin utilization that is mediated by TfoX; and pyrimidine starvation that is orchestrated by CytR, toward each of these processes. We find that in V. cholerae, CytR is a global regulator of multiple behaviors affecting fitness and adaptability in the environment.

No MeSH data available.


Related in: MedlinePlus

The differential roles of TfoX, CytR, HapR and QstR in natural transformation, Type VI secretion and chitinase expression.Natural transformation requires inputs from four regulators TfoX, CytR, HapR and QstR. Type VI secretion requires inputs from the four regulators above, but QstR overexpression bypasses the need for TfoX, CytR, and HapR. Chitinase expression requires inputs from TfoX, CytR but not from HapR and QstR under the conditions tested.
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pone.0138834.g005: The differential roles of TfoX, CytR, HapR and QstR in natural transformation, Type VI secretion and chitinase expression.Natural transformation requires inputs from four regulators TfoX, CytR, HapR and QstR. Type VI secretion requires inputs from the four regulators above, but QstR overexpression bypasses the need for TfoX, CytR, and HapR. Chitinase expression requires inputs from TfoX, CytR but not from HapR and QstR under the conditions tested.

Mentions: We have demonstrated that in V. cholerae, the regulator CytR not only represses genes involved in nucleoside metabolism and transport, but also positively regulates natural transformation, the type VI secretion system, and chitin degradation indicating novel roles for CytR regulation. Although each of these phenotypes requires CytR for expression, the specific mechanism of regulation and the involvement of other transcription factors appear to differ in each case (Fig 5).


CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae.

Watve SS, Thomas J, Hammer BK - PLoS ONE (2015)

The differential roles of TfoX, CytR, HapR and QstR in natural transformation, Type VI secretion and chitinase expression.Natural transformation requires inputs from four regulators TfoX, CytR, HapR and QstR. Type VI secretion requires inputs from the four regulators above, but QstR overexpression bypasses the need for TfoX, CytR, and HapR. Chitinase expression requires inputs from TfoX, CytR but not from HapR and QstR under the conditions tested.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581735&req=5

pone.0138834.g005: The differential roles of TfoX, CytR, HapR and QstR in natural transformation, Type VI secretion and chitinase expression.Natural transformation requires inputs from four regulators TfoX, CytR, HapR and QstR. Type VI secretion requires inputs from the four regulators above, but QstR overexpression bypasses the need for TfoX, CytR, and HapR. Chitinase expression requires inputs from TfoX, CytR but not from HapR and QstR under the conditions tested.
Mentions: We have demonstrated that in V. cholerae, the regulator CytR not only represses genes involved in nucleoside metabolism and transport, but also positively regulates natural transformation, the type VI secretion system, and chitin degradation indicating novel roles for CytR regulation. Although each of these phenotypes requires CytR for expression, the specific mechanism of regulation and the involvement of other transcription factors appear to differ in each case (Fig 5).

Bottom Line: Through high-throughput RNA sequencing (RNA-seq), we show that CytR positively regulates the majority of competence genes, the three type VI secretion operons, and the four known or predicted chitinases.We used transcriptional reporters and phenotypic analysis to determine the individual contributions of quorum sensing, which is controlled by the transcription factors HapR and QstR; chitin utilization that is mediated by TfoX; and pyrimidine starvation that is orchestrated by CytR, toward each of these processes.We find that in V. cholerae, CytR is a global regulator of multiple behaviors affecting fitness and adaptability in the environment.

View Article: PubMed Central - PubMed

Affiliation: School of Biology, Georgia Institute of Technology, Atlanta, Georgia, United States of America.

ABSTRACT
The facultative pathogen Vibrio cholerae transitions between its human host and aquatic reservoirs where it colonizes chitinous surfaces. Growth on chitin induces expression of chitin utilization genes, genes involved in DNA uptake by natural transformation, and a type VI secretion system that allows contact-dependent killing of neighboring bacteria. We have previously shown that the transcription factor CytR, thought to primarily regulate the pyrimidine nucleoside scavenging response, is required for natural competence in V. cholerae. Through high-throughput RNA sequencing (RNA-seq), we show that CytR positively regulates the majority of competence genes, the three type VI secretion operons, and the four known or predicted chitinases. We used transcriptional reporters and phenotypic analysis to determine the individual contributions of quorum sensing, which is controlled by the transcription factors HapR and QstR; chitin utilization that is mediated by TfoX; and pyrimidine starvation that is orchestrated by CytR, toward each of these processes. We find that in V. cholerae, CytR is a global regulator of multiple behaviors affecting fitness and adaptability in the environment.

No MeSH data available.


Related in: MedlinePlus