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The Effect of Mesenchymal Stem Cells and Chitosan Gel on Full Thickness Skin Wound Healing in Albino Rats: Histological, Immunohistochemical and Fluorescent Study.

El Sadik AO, El Ghamrawy TA, Abd El-Galil TI - PLoS ONE (2015)

Bottom Line: Both chitosan and mesenchymal stem cells (MSCs) were used in treating skin wounds.Collagen fibers were arranged in many directions, with significant increase in their area percent, surrounding fully regenerated hair follicles and sebaceous glands in the dermis of the healed areas more than in other groups.MSCs enhanced the healing process of wound closure more than chitosan gel treatment.

View Article: PubMed Central - PubMed

Affiliation: Anatomy and Embryology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT

Background: Wound healing involves the integration of complex biological processes. Several studies examined numerous approaches to enhance wound healing and to minimize its related morbidity. Both chitosan and mesenchymal stem cells (MSCs) were used in treating skin wounds. The aim of the current work was to compare MSCs versus chitosan in wound healing, evaluate the most efficient route of administration of MSCs, either intradermal or systemic injection, and elicit the mechanisms inducing epidermal and dermal cell regeneration using histological, immunohistochemical and fluorescent techniques.

Material and methods: Forty adult male Sprague Dawley albino rats were divided into four equal groups (ten rats in each group): control group (Group I); full thickness surgical skin wound model, Group II: Wound and chitosan gel. Group III: Wound treated with systemic injection of MSCs and Group IV: Wound treated with intradermal injection of MSCs. The healing ulcer was examined on day 3, 5, 10 and 15 for gross morphological evaluation and on day 10 and 15 for histological, immunohistochemical and fluorescent studies.

Results: Chitosan was proved to promote wound healing more than the control group but none of their wound reached complete closure. Better and faster healing of wounds in MSCs treated groups were manifested more than the control or chitosan treated groups. It was found that the intradermal route of administration of stem cells enhanced the rate of healing of skin wounds better than the systemic administration to the extent that, by the end of the fifteenth day of the experiment, the wounds were completely healed in all rats of this group. Histologically, the wound areas of group IV were hardly demarcated from the adjacent normal skin and showed complete regeneration of the epidermis, dermis, hypodermis and underlying muscle fibers. Collagen fibers were arranged in many directions, with significant increase in their area percent, surrounding fully regenerated hair follicles and sebaceous glands in the dermis of the healed areas more than in other groups.

Conclusion: MSCs enhanced the healing process of wound closure more than chitosan gel treatment. Furthermore, MSCs injected intradermally, were more efficient in accelerating wound healing than any other mode of treatment.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of the skin wound area on the 10th day.a) Group III showing red fluorescent cells (arrows) within the proliferated granulation tissue in the ulcer. b) Group IV showing fluorescent cells (arrows) within the proliferated granulation tissue, the new regenerated epidermis (E) and new blood vessels (V). By the 15th day: c) Group III showing increasing area of fluorescent cells within the regenerated epidermis (E) and within the regenerated dermis (D) than day 10 groups. d) Group IV showed the highest distribution than previous groups of fluorescent cells within the regenerated epidermis (E), the regenerated dermis (D), blood vessels (V) and hair follicle (F). (PKH, x100)
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pone.0137544.g005: Photomicrographs of the skin wound area on the 10th day.a) Group III showing red fluorescent cells (arrows) within the proliferated granulation tissue in the ulcer. b) Group IV showing fluorescent cells (arrows) within the proliferated granulation tissue, the new regenerated epidermis (E) and new blood vessels (V). By the 15th day: c) Group III showing increasing area of fluorescent cells within the regenerated epidermis (E) and within the regenerated dermis (D) than day 10 groups. d) Group IV showed the highest distribution than previous groups of fluorescent cells within the regenerated epidermis (E), the regenerated dermis (D), blood vessels (V) and hair follicle (F). (PKH, x100)

Mentions: On the 10th day, MSCs systemically treated group (group III) showed PKH labelled red fluorescent cells within the proliferated granulation tissue of the wound area (Fig 5a). Intradermally injected MSCs group (group IV) had red fluorescent cells within the granulation tissue, in addition to the new regenerated epidermis and the new blood vessels within the wound area (Fig 5b). By the 15th day, group III revealed increased labelled fluorescent cells compared to day 10 within the regenerated epidermis in addition to its dermis (Fig 5c). Group IV had the highest labelled cells within the regenerated epidermis, dermis, blood vessels and hair follicles, compared to the other group (Fig 5d).


The Effect of Mesenchymal Stem Cells and Chitosan Gel on Full Thickness Skin Wound Healing in Albino Rats: Histological, Immunohistochemical and Fluorescent Study.

El Sadik AO, El Ghamrawy TA, Abd El-Galil TI - PLoS ONE (2015)

Photomicrographs of the skin wound area on the 10th day.a) Group III showing red fluorescent cells (arrows) within the proliferated granulation tissue in the ulcer. b) Group IV showing fluorescent cells (arrows) within the proliferated granulation tissue, the new regenerated epidermis (E) and new blood vessels (V). By the 15th day: c) Group III showing increasing area of fluorescent cells within the regenerated epidermis (E) and within the regenerated dermis (D) than day 10 groups. d) Group IV showed the highest distribution than previous groups of fluorescent cells within the regenerated epidermis (E), the regenerated dermis (D), blood vessels (V) and hair follicle (F). (PKH, x100)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581728&req=5

pone.0137544.g005: Photomicrographs of the skin wound area on the 10th day.a) Group III showing red fluorescent cells (arrows) within the proliferated granulation tissue in the ulcer. b) Group IV showing fluorescent cells (arrows) within the proliferated granulation tissue, the new regenerated epidermis (E) and new blood vessels (V). By the 15th day: c) Group III showing increasing area of fluorescent cells within the regenerated epidermis (E) and within the regenerated dermis (D) than day 10 groups. d) Group IV showed the highest distribution than previous groups of fluorescent cells within the regenerated epidermis (E), the regenerated dermis (D), blood vessels (V) and hair follicle (F). (PKH, x100)
Mentions: On the 10th day, MSCs systemically treated group (group III) showed PKH labelled red fluorescent cells within the proliferated granulation tissue of the wound area (Fig 5a). Intradermally injected MSCs group (group IV) had red fluorescent cells within the granulation tissue, in addition to the new regenerated epidermis and the new blood vessels within the wound area (Fig 5b). By the 15th day, group III revealed increased labelled fluorescent cells compared to day 10 within the regenerated epidermis in addition to its dermis (Fig 5c). Group IV had the highest labelled cells within the regenerated epidermis, dermis, blood vessels and hair follicles, compared to the other group (Fig 5d).

Bottom Line: Both chitosan and mesenchymal stem cells (MSCs) were used in treating skin wounds.Collagen fibers were arranged in many directions, with significant increase in their area percent, surrounding fully regenerated hair follicles and sebaceous glands in the dermis of the healed areas more than in other groups.MSCs enhanced the healing process of wound closure more than chitosan gel treatment.

View Article: PubMed Central - PubMed

Affiliation: Anatomy and Embryology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT

Background: Wound healing involves the integration of complex biological processes. Several studies examined numerous approaches to enhance wound healing and to minimize its related morbidity. Both chitosan and mesenchymal stem cells (MSCs) were used in treating skin wounds. The aim of the current work was to compare MSCs versus chitosan in wound healing, evaluate the most efficient route of administration of MSCs, either intradermal or systemic injection, and elicit the mechanisms inducing epidermal and dermal cell regeneration using histological, immunohistochemical and fluorescent techniques.

Material and methods: Forty adult male Sprague Dawley albino rats were divided into four equal groups (ten rats in each group): control group (Group I); full thickness surgical skin wound model, Group II: Wound and chitosan gel. Group III: Wound treated with systemic injection of MSCs and Group IV: Wound treated with intradermal injection of MSCs. The healing ulcer was examined on day 3, 5, 10 and 15 for gross morphological evaluation and on day 10 and 15 for histological, immunohistochemical and fluorescent studies.

Results: Chitosan was proved to promote wound healing more than the control group but none of their wound reached complete closure. Better and faster healing of wounds in MSCs treated groups were manifested more than the control or chitosan treated groups. It was found that the intradermal route of administration of stem cells enhanced the rate of healing of skin wounds better than the systemic administration to the extent that, by the end of the fifteenth day of the experiment, the wounds were completely healed in all rats of this group. Histologically, the wound areas of group IV were hardly demarcated from the adjacent normal skin and showed complete regeneration of the epidermis, dermis, hypodermis and underlying muscle fibers. Collagen fibers were arranged in many directions, with significant increase in their area percent, surrounding fully regenerated hair follicles and sebaceous glands in the dermis of the healed areas more than in other groups.

Conclusion: MSCs enhanced the healing process of wound closure more than chitosan gel treatment. Furthermore, MSCs injected intradermally, were more efficient in accelerating wound healing than any other mode of treatment.

No MeSH data available.


Related in: MedlinePlus