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Changes in Variable Number of Tandem Repeats in 'Candidatus Liberibacter asiaticus' through Insect Transmission.

Katoh H, Inoue H, Iwanami T - PLoS ONE (2015)

Bottom Line: The disease is associated with three species of 'Candidatus Liberibacter' among which 'Ca.The results showed that some tandem repeats showed detectable changes after insect transmission.In contrast, changes in the number of tandem repeats increased with longer acquisition and inoculation access periods, whereas changes were not observed through psyllid transmission after relatively short acquisition and inoculation access periods, up to 20 and 19 days, respectively.

View Article: PubMed Central - PubMed

Affiliation: NARO Institute of Fruit Tree Science, Fujimoto 2-1, Tsukuba, Ibaraki 305-8605, Japan.

ABSTRACT
Citrus greening (huanglongbing) is the most destructive citrus disease worldwide. The disease is associated with three species of 'Candidatus Liberibacter' among which 'Ca. Liberibacter asiaticus' has the widest distribution. 'Ca. L. asiaticus' is commonly transmitted by a phloem-feeding insect vector, the Asian citrus psyllid Diaphorina citri. A previous study showed that isolates of 'Ca. L. asiaticus' were clearly differentiated by variable number of tandem repeat (VNTR) profiles at four loci in the genome. In this study, the VNTR analysis was further validated by assessing the stability of these repeats after multiplication of the pathogen upon host-to-host transmission using a 'Ca. L. asiaticus' strain from Japan. The results showed that some tandem repeats showed detectable changes after insect transmission. To our knowledge, this is the first report to demonstrate that the repeat numbers VNTR 002 and 077 of 'Ca. L. asiaticus' change through psyllid transmission. VNTRs in the recipient plant were apparently unrelated to the growing phase of the vector. In contrast, changes in the number of tandem repeats increased with longer acquisition and inoculation access periods, whereas changes were not observed through psyllid transmission after relatively short acquisition and inoculation access periods, up to 20 and 19 days, respectively.

No MeSH data available.


Related in: MedlinePlus

Schematic model of strategy of Table 3.The black circle indicates the area used for DNA extraction. Numbers at the lower right, which are x n, indicate the number of samples in Table 3.
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pone.0138699.g002: Schematic model of strategy of Table 3.The black circle indicates the area used for DNA extraction. Numbers at the lower right, which are x n, indicate the number of samples in Table 3.

Mentions: Changes in VNTRs in the ‘Ca. L. asiaticus’ genome occurred while the bacterium was in the psyllid body (Table 3, Fig 2). These VNTR changes in the psyllid body might have contributed to the changes of VNTRs in the ‘Ca. L. asiaticus’ genome in the recipient plant in some cases. For example, the profile of VNTRs of ‘Ca. L. asiaticus’ in one yuzu recipient plant (sample ID: Recipient-Y17) perfectly matches the VNTRs of ‘Ca. L. asiaticus’ in the psyllid body parts (Table 3).


Changes in Variable Number of Tandem Repeats in 'Candidatus Liberibacter asiaticus' through Insect Transmission.

Katoh H, Inoue H, Iwanami T - PLoS ONE (2015)

Schematic model of strategy of Table 3.The black circle indicates the area used for DNA extraction. Numbers at the lower right, which are x n, indicate the number of samples in Table 3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581716&req=5

pone.0138699.g002: Schematic model of strategy of Table 3.The black circle indicates the area used for DNA extraction. Numbers at the lower right, which are x n, indicate the number of samples in Table 3.
Mentions: Changes in VNTRs in the ‘Ca. L. asiaticus’ genome occurred while the bacterium was in the psyllid body (Table 3, Fig 2). These VNTR changes in the psyllid body might have contributed to the changes of VNTRs in the ‘Ca. L. asiaticus’ genome in the recipient plant in some cases. For example, the profile of VNTRs of ‘Ca. L. asiaticus’ in one yuzu recipient plant (sample ID: Recipient-Y17) perfectly matches the VNTRs of ‘Ca. L. asiaticus’ in the psyllid body parts (Table 3).

Bottom Line: The disease is associated with three species of 'Candidatus Liberibacter' among which 'Ca.The results showed that some tandem repeats showed detectable changes after insect transmission.In contrast, changes in the number of tandem repeats increased with longer acquisition and inoculation access periods, whereas changes were not observed through psyllid transmission after relatively short acquisition and inoculation access periods, up to 20 and 19 days, respectively.

View Article: PubMed Central - PubMed

Affiliation: NARO Institute of Fruit Tree Science, Fujimoto 2-1, Tsukuba, Ibaraki 305-8605, Japan.

ABSTRACT
Citrus greening (huanglongbing) is the most destructive citrus disease worldwide. The disease is associated with three species of 'Candidatus Liberibacter' among which 'Ca. Liberibacter asiaticus' has the widest distribution. 'Ca. L. asiaticus' is commonly transmitted by a phloem-feeding insect vector, the Asian citrus psyllid Diaphorina citri. A previous study showed that isolates of 'Ca. L. asiaticus' were clearly differentiated by variable number of tandem repeat (VNTR) profiles at four loci in the genome. In this study, the VNTR analysis was further validated by assessing the stability of these repeats after multiplication of the pathogen upon host-to-host transmission using a 'Ca. L. asiaticus' strain from Japan. The results showed that some tandem repeats showed detectable changes after insect transmission. To our knowledge, this is the first report to demonstrate that the repeat numbers VNTR 002 and 077 of 'Ca. L. asiaticus' change through psyllid transmission. VNTRs in the recipient plant were apparently unrelated to the growing phase of the vector. In contrast, changes in the number of tandem repeats increased with longer acquisition and inoculation access periods, whereas changes were not observed through psyllid transmission after relatively short acquisition and inoculation access periods, up to 20 and 19 days, respectively.

No MeSH data available.


Related in: MedlinePlus