Limits...
Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice.

Zhao Y, Liu F, Liu Y, Zhou D, Dai Q, Liu S - PLoS ONE (2015)

Bottom Line: To date, there are no effective therapies to slow the progress of this degenerative condition.Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion.Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate the anti-arthritic effect of chebulanin, an abundant anti-inflammatory agent isolated from Terminalia chebula, in collagen induced arthritis in DBA/1 mice by intragastric administration. Arthritic severity was scored by performing histopathological evaluation of the joints and measuring the expression of inflammatory cytokines and relative enzymes by immunohistochemical staining. In parallel, bone destruction and erosion were confirmed by micro-CT. Our data revealed that chebulanin significantly improved the severity of arthritis. Specifically, the histopathological characteristics of the tissues were improved and expression of TNF-α, IL-6, MMP-3 and COX-2 in the paws and joints of the treated mice decreased in a dose-dependent manner compared with control mice. Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion. Taken together, our findings suggest that chebulanin suppresses the expression of inflammatory mediators and prevents cartilage destruction and bone erosion in mice. Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

No MeSH data available.


Related in: MedlinePlus

Chebulanin treatment decreases the expression of the inflammation- related enzymes COX-2 and MMP-3 in CIA mice.Immunohistochemical staining of (A) COX-2 and (B) MMP-3 expression in the hind paws of treated and control mice and semi-quantitative analysis of the staining score. Photomicrographs were obtained at 40x magnification and data are expressed as mean ± standard deviation (n = 6). *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581703&req=5

pone.0139052.g006: Chebulanin treatment decreases the expression of the inflammation- related enzymes COX-2 and MMP-3 in CIA mice.Immunohistochemical staining of (A) COX-2 and (B) MMP-3 expression in the hind paws of treated and control mice and semi-quantitative analysis of the staining score. Photomicrographs were obtained at 40x magnification and data are expressed as mean ± standard deviation (n = 6). *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.

Mentions: Similar results were obtained for COX-2 (Fig 6A) and MMP-3 (Fig 6B) expression. Semi-quantitative pathological analysis confirmed that the expression levels for these two markers were markedly reduced in chebulanin-treated mice compared to untreated CIA mice and that this effect was dose-dependent.


Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice.

Zhao Y, Liu F, Liu Y, Zhou D, Dai Q, Liu S - PLoS ONE (2015)

Chebulanin treatment decreases the expression of the inflammation- related enzymes COX-2 and MMP-3 in CIA mice.Immunohistochemical staining of (A) COX-2 and (B) MMP-3 expression in the hind paws of treated and control mice and semi-quantitative analysis of the staining score. Photomicrographs were obtained at 40x magnification and data are expressed as mean ± standard deviation (n = 6). *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581703&req=5

pone.0139052.g006: Chebulanin treatment decreases the expression of the inflammation- related enzymes COX-2 and MMP-3 in CIA mice.Immunohistochemical staining of (A) COX-2 and (B) MMP-3 expression in the hind paws of treated and control mice and semi-quantitative analysis of the staining score. Photomicrographs were obtained at 40x magnification and data are expressed as mean ± standard deviation (n = 6). *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
Mentions: Similar results were obtained for COX-2 (Fig 6A) and MMP-3 (Fig 6B) expression. Semi-quantitative pathological analysis confirmed that the expression levels for these two markers were markedly reduced in chebulanin-treated mice compared to untreated CIA mice and that this effect was dose-dependent.

Bottom Line: To date, there are no effective therapies to slow the progress of this degenerative condition.Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion.Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate the anti-arthritic effect of chebulanin, an abundant anti-inflammatory agent isolated from Terminalia chebula, in collagen induced arthritis in DBA/1 mice by intragastric administration. Arthritic severity was scored by performing histopathological evaluation of the joints and measuring the expression of inflammatory cytokines and relative enzymes by immunohistochemical staining. In parallel, bone destruction and erosion were confirmed by micro-CT. Our data revealed that chebulanin significantly improved the severity of arthritis. Specifically, the histopathological characteristics of the tissues were improved and expression of TNF-α, IL-6, MMP-3 and COX-2 in the paws and joints of the treated mice decreased in a dose-dependent manner compared with control mice. Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion. Taken together, our findings suggest that chebulanin suppresses the expression of inflammatory mediators and prevents cartilage destruction and bone erosion in mice. Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

No MeSH data available.


Related in: MedlinePlus