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Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice.

Zhao Y, Liu F, Liu Y, Zhou D, Dai Q, Liu S - PLoS ONE (2015)

Bottom Line: To date, there are no effective therapies to slow the progress of this degenerative condition.Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion.Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate the anti-arthritic effect of chebulanin, an abundant anti-inflammatory agent isolated from Terminalia chebula, in collagen induced arthritis in DBA/1 mice by intragastric administration. Arthritic severity was scored by performing histopathological evaluation of the joints and measuring the expression of inflammatory cytokines and relative enzymes by immunohistochemical staining. In parallel, bone destruction and erosion were confirmed by micro-CT. Our data revealed that chebulanin significantly improved the severity of arthritis. Specifically, the histopathological characteristics of the tissues were improved and expression of TNF-α, IL-6, MMP-3 and COX-2 in the paws and joints of the treated mice decreased in a dose-dependent manner compared with control mice. Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion. Taken together, our findings suggest that chebulanin suppresses the expression of inflammatory mediators and prevents cartilage destruction and bone erosion in mice. Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

No MeSH data available.


Related in: MedlinePlus

Hind paws of chebulanin-treated mice show improved bone architecture.H&E stained histopathological images of the hind paws from chebulanin-treated and control mice (40x). Semi-quantitative analysis of histopathological findings of the inflamed joints. Data are expressed as mean ± standard deviation(n = 6). *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
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pone.0139052.g004: Hind paws of chebulanin-treated mice show improved bone architecture.H&E stained histopathological images of the hind paws from chebulanin-treated and control mice (40x). Semi-quantitative analysis of histopathological findings of the inflamed joints. Data are expressed as mean ± standard deviation(n = 6). *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.

Mentions: Histopathological assessment of paws and joints from treated and control mice revealed remarkable signs of cellular infiltration, synovial hyperplasia, pannus formation, partial cartilage and bone destruction in the untreated CIA mice. However, chebulanin-treated mice had significantly decreased levels of cellular infiltration, hyperplasia and bone destruction and this improved histopathology was dose-dependent. The semi-quantitative analysis of histopathological findings including infiltration and erosion scores is shown in Fig 4.


Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice.

Zhao Y, Liu F, Liu Y, Zhou D, Dai Q, Liu S - PLoS ONE (2015)

Hind paws of chebulanin-treated mice show improved bone architecture.H&E stained histopathological images of the hind paws from chebulanin-treated and control mice (40x). Semi-quantitative analysis of histopathological findings of the inflamed joints. Data are expressed as mean ± standard deviation(n = 6). *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4581703&req=5

pone.0139052.g004: Hind paws of chebulanin-treated mice show improved bone architecture.H&E stained histopathological images of the hind paws from chebulanin-treated and control mice (40x). Semi-quantitative analysis of histopathological findings of the inflamed joints. Data are expressed as mean ± standard deviation(n = 6). *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
Mentions: Histopathological assessment of paws and joints from treated and control mice revealed remarkable signs of cellular infiltration, synovial hyperplasia, pannus formation, partial cartilage and bone destruction in the untreated CIA mice. However, chebulanin-treated mice had significantly decreased levels of cellular infiltration, hyperplasia and bone destruction and this improved histopathology was dose-dependent. The semi-quantitative analysis of histopathological findings including infiltration and erosion scores is shown in Fig 4.

Bottom Line: To date, there are no effective therapies to slow the progress of this degenerative condition.Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion.Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate the anti-arthritic effect of chebulanin, an abundant anti-inflammatory agent isolated from Terminalia chebula, in collagen induced arthritis in DBA/1 mice by intragastric administration. Arthritic severity was scored by performing histopathological evaluation of the joints and measuring the expression of inflammatory cytokines and relative enzymes by immunohistochemical staining. In parallel, bone destruction and erosion were confirmed by micro-CT. Our data revealed that chebulanin significantly improved the severity of arthritis. Specifically, the histopathological characteristics of the tissues were improved and expression of TNF-α, IL-6, MMP-3 and COX-2 in the paws and joints of the treated mice decreased in a dose-dependent manner compared with control mice. Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion. Taken together, our findings suggest that chebulanin suppresses the expression of inflammatory mediators and prevents cartilage destruction and bone erosion in mice. Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

No MeSH data available.


Related in: MedlinePlus