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Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice.

Zhao Y, Liu F, Liu Y, Zhou D, Dai Q, Liu S - PLoS ONE (2015)

Bottom Line: To date, there are no effective therapies to slow the progress of this degenerative condition.Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion.Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate the anti-arthritic effect of chebulanin, an abundant anti-inflammatory agent isolated from Terminalia chebula, in collagen induced arthritis in DBA/1 mice by intragastric administration. Arthritic severity was scored by performing histopathological evaluation of the joints and measuring the expression of inflammatory cytokines and relative enzymes by immunohistochemical staining. In parallel, bone destruction and erosion were confirmed by micro-CT. Our data revealed that chebulanin significantly improved the severity of arthritis. Specifically, the histopathological characteristics of the tissues were improved and expression of TNF-α, IL-6, MMP-3 and COX-2 in the paws and joints of the treated mice decreased in a dose-dependent manner compared with control mice. Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion. Taken together, our findings suggest that chebulanin suppresses the expression of inflammatory mediators and prevents cartilage destruction and bone erosion in mice. Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

No MeSH data available.


Related in: MedlinePlus

3D reconstruction of micro CT images of the hind paws and relative parameters of bone integrity.A: Representative 3D-micro computed tomography images of the hind paws showing joints from normal mice having smooth bone surface with intact joint architecture, and joints from CIA mice having significant bone erosion. B: Parameters of bone integrity. BV: bone volume; BS/BV: bone surface area/bone volume; Tb.Th: trabecular thickness. Data are expressed as mean ±standard deviation(n = 6); *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
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pone.0139052.g003: 3D reconstruction of micro CT images of the hind paws and relative parameters of bone integrity.A: Representative 3D-micro computed tomography images of the hind paws showing joints from normal mice having smooth bone surface with intact joint architecture, and joints from CIA mice having significant bone erosion. B: Parameters of bone integrity. BV: bone volume; BS/BV: bone surface area/bone volume; Tb.Th: trabecular thickness. Data are expressed as mean ±standard deviation(n = 6); *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.

Mentions: The severity of joint destruction was assessed using 3D micro-CT (Fig 3). The paws of normal mice had a smooth bone surface, while those from untreated CIA mice had severe bone erosions. Importantly, chebulanin-treated CIA mice showed markedly less joint destruction, especially at the mid- and high-treatment doses of 80 mg/kg and 160 mg/kg.


Anti-Arthritic Effect of Chebulanin on Collagen-Induced Arthritis in Mice.

Zhao Y, Liu F, Liu Y, Zhou D, Dai Q, Liu S - PLoS ONE (2015)

3D reconstruction of micro CT images of the hind paws and relative parameters of bone integrity.A: Representative 3D-micro computed tomography images of the hind paws showing joints from normal mice having smooth bone surface with intact joint architecture, and joints from CIA mice having significant bone erosion. B: Parameters of bone integrity. BV: bone volume; BS/BV: bone surface area/bone volume; Tb.Th: trabecular thickness. Data are expressed as mean ±standard deviation(n = 6); *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581703&req=5

pone.0139052.g003: 3D reconstruction of micro CT images of the hind paws and relative parameters of bone integrity.A: Representative 3D-micro computed tomography images of the hind paws showing joints from normal mice having smooth bone surface with intact joint architecture, and joints from CIA mice having significant bone erosion. B: Parameters of bone integrity. BV: bone volume; BS/BV: bone surface area/bone volume; Tb.Th: trabecular thickness. Data are expressed as mean ±standard deviation(n = 6); *p <0.05 vs. untreated CIA mice, #p <0.05 vs. normal mice.
Mentions: The severity of joint destruction was assessed using 3D micro-CT (Fig 3). The paws of normal mice had a smooth bone surface, while those from untreated CIA mice had severe bone erosions. Importantly, chebulanin-treated CIA mice showed markedly less joint destruction, especially at the mid- and high-treatment doses of 80 mg/kg and 160 mg/kg.

Bottom Line: To date, there are no effective therapies to slow the progress of this degenerative condition.Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion.Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT
Rheumatoid arthritis is a chronic degenerative autoimmune disease characterized by persistent inflammation of synovial membranes, which leads to cartilage destruction and bone erosion. To date, there are no effective therapies to slow the progress of this degenerative condition. Here, we evaluate the anti-arthritic effect of chebulanin, an abundant anti-inflammatory agent isolated from Terminalia chebula, in collagen induced arthritis in DBA/1 mice by intragastric administration. Arthritic severity was scored by performing histopathological evaluation of the joints and measuring the expression of inflammatory cytokines and relative enzymes by immunohistochemical staining. In parallel, bone destruction and erosion were confirmed by micro-CT. Our data revealed that chebulanin significantly improved the severity of arthritis. Specifically, the histopathological characteristics of the tissues were improved and expression of TNF-α, IL-6, MMP-3 and COX-2 in the paws and joints of the treated mice decreased in a dose-dependent manner compared with control mice. Furthermore, micro-CT analysis revealed that chebulanin induced a dose-dependent reduction in cartilage destruction and bone erosion. Taken together, our findings suggest that chebulanin suppresses the expression of inflammatory mediators and prevents cartilage destruction and bone erosion in mice. Therefore, chebulanin is a strong therapeutic alternative for the treatment of RA.

No MeSH data available.


Related in: MedlinePlus