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Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus

Expression analysis of co-regulated genes related to network modulated by expression of Os08g01480 in Arabidopsis.qRT-PCR analysis was carried out in two week old seedlings of WT and transgenic lines grown in ½ MS media.
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pone.0138574.g006: Expression analysis of co-regulated genes related to network modulated by expression of Os08g01480 in Arabidopsis.qRT-PCR analysis was carried out in two week old seedlings of WT and transgenic lines grown in ½ MS media.

Mentions: To elucidate the rationale behind tolerance of transgenic lines to different abiotic stresses, expression of co-regulated genes which are known to help in defense, growth and development of plants were examined. An enhanced expression of HY5, GBF3, EDS1, COI1, YUC4, CYP707A1, CYP72C1, AOS, PAD4, BRI1, CYP82C2 and ER was observed in transgenic lines in comparison to WT (Fig 6). Enhanced expression of these genes seems to be possible reason for tolerance of transgenic lines against different abiotic stresses.


Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Expression analysis of co-regulated genes related to network modulated by expression of Os08g01480 in Arabidopsis.qRT-PCR analysis was carried out in two week old seedlings of WT and transgenic lines grown in ½ MS media.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581635&req=5

pone.0138574.g006: Expression analysis of co-regulated genes related to network modulated by expression of Os08g01480 in Arabidopsis.qRT-PCR analysis was carried out in two week old seedlings of WT and transgenic lines grown in ½ MS media.
Mentions: To elucidate the rationale behind tolerance of transgenic lines to different abiotic stresses, expression of co-regulated genes which are known to help in defense, growth and development of plants were examined. An enhanced expression of HY5, GBF3, EDS1, COI1, YUC4, CYP707A1, CYP72C1, AOS, PAD4, BRI1, CYP82C2 and ER was observed in transgenic lines in comparison to WT (Fig 6). Enhanced expression of these genes seems to be possible reason for tolerance of transgenic lines against different abiotic stresses.

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus