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Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus

Pathway studio analysis used for prediction of molecular network modulated by expression of CYP like gene Os08g01480 in Arabidopsis.Pathway studio is utilized for finding common molecular connections between the proteins/transcription factors encoded by the expressed probe sets. This software analyses through the ResNet database for all known interactions between genes/proteins such as regulation and their expression. Each arrow indicates interactions between genes and a cell process pathway.
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pone.0138574.g005: Pathway studio analysis used for prediction of molecular network modulated by expression of CYP like gene Os08g01480 in Arabidopsis.Pathway studio is utilized for finding common molecular connections between the proteins/transcription factors encoded by the expressed probe sets. This software analyses through the ResNet database for all known interactions between genes/proteins such as regulation and their expression. Each arrow indicates interactions between genes and a cell process pathway.

Mentions: The predicted network (Fig 5) shows that the genes involved in many cellular processes like defense response, leaf development, root growth, flower development, embyronal development, hypocotyls growth, stomatal movement, auxin metabolism, cell elongation, cytokinesis, apoptosis and light response etc. are associated by Os08g01480. Genes related to defense response such as COI1, BRI1 and AOS were observed in the networks which are modulated during expression of Os08g01480 in Arabidopsis. This suggests that these genes might be playing a role in improved defense response of transgenic lines against different abiotic stress. Auxin stimulus responsive genes (HY5 and GBF) and gene related to systemic acquired resistance (SAR), AOS, was also involved in network. These genes play important role in improving plant defense mechanism and help the plant to combat abiotic stress. EDS1, COI1, YUC4 and ER in flower development, COI1 and BRI1 in cell elongation, CYP707A1 in stomatal movement, EDS1 in apoptosis and AOS, CYP82C2, COI1 and HY5 in root growth are few genes predicted in pathway known to be involved in the growth and development of the plants.


Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Pathway studio analysis used for prediction of molecular network modulated by expression of CYP like gene Os08g01480 in Arabidopsis.Pathway studio is utilized for finding common molecular connections between the proteins/transcription factors encoded by the expressed probe sets. This software analyses through the ResNet database for all known interactions between genes/proteins such as regulation and their expression. Each arrow indicates interactions between genes and a cell process pathway.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581635&req=5

pone.0138574.g005: Pathway studio analysis used for prediction of molecular network modulated by expression of CYP like gene Os08g01480 in Arabidopsis.Pathway studio is utilized for finding common molecular connections between the proteins/transcription factors encoded by the expressed probe sets. This software analyses through the ResNet database for all known interactions between genes/proteins such as regulation and their expression. Each arrow indicates interactions between genes and a cell process pathway.
Mentions: The predicted network (Fig 5) shows that the genes involved in many cellular processes like defense response, leaf development, root growth, flower development, embyronal development, hypocotyls growth, stomatal movement, auxin metabolism, cell elongation, cytokinesis, apoptosis and light response etc. are associated by Os08g01480. Genes related to defense response such as COI1, BRI1 and AOS were observed in the networks which are modulated during expression of Os08g01480 in Arabidopsis. This suggests that these genes might be playing a role in improved defense response of transgenic lines against different abiotic stress. Auxin stimulus responsive genes (HY5 and GBF) and gene related to systemic acquired resistance (SAR), AOS, was also involved in network. These genes play important role in improving plant defense mechanism and help the plant to combat abiotic stress. EDS1, COI1, YUC4 and ER in flower development, COI1 and BRI1 in cell elongation, CYP707A1 in stomatal movement, EDS1 in apoptosis and AOS, CYP82C2, COI1 and HY5 in root growth are few genes predicted in pathway known to be involved in the growth and development of the plants.

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus