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Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus

Chlorophyll fluorescence imaging (Fv/Fm) of WT and transgenic line (L1) was performed during control and stress conditions.Fv/Fm of non-treated WT and transgenic was carried out after 14 days of germination. Fv/Fm was carried out for WT and transgenic line after they were exposed to 37°C and 0°C for 4 h and again after10 and 15d recovery from heat and cold stress, respectively. For salt and different HM stress WT and transgenic line were watered with nutrient media containing NaCl (300 mM), 100 μM As(III), 200 μM As(V), 200 μM Cd and 400 μM Cr(VI) for two weeks and then Fv/Fm were measured. For osmotic stress WT and transgenic line were withheld from water for 14 d, and then rewatered for 7 d before Fv/Fm was measured. The false color code depicted on the right side of images ranges from 0 (black) to 1 (pink) Level of significance is considered as P≤0.05.
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pone.0138574.g004: Chlorophyll fluorescence imaging (Fv/Fm) of WT and transgenic line (L1) was performed during control and stress conditions.Fv/Fm of non-treated WT and transgenic was carried out after 14 days of germination. Fv/Fm was carried out for WT and transgenic line after they were exposed to 37°C and 0°C for 4 h and again after10 and 15d recovery from heat and cold stress, respectively. For salt and different HM stress WT and transgenic line were watered with nutrient media containing NaCl (300 mM), 100 μM As(III), 200 μM As(V), 200 μM Cd and 400 μM Cr(VI) for two weeks and then Fv/Fm were measured. For osmotic stress WT and transgenic line were withheld from water for 14 d, and then rewatered for 7 d before Fv/Fm was measured. The false color code depicted on the right side of images ranges from 0 (black) to 1 (pink) Level of significance is considered as P≤0.05.

Mentions: Fluorescence dynamics for cold and heat stress were performed immediately after WT and transgenic lines were kept at 0°C and 37°C for 4 h and then after 10 d and 15 d of recovery under simultaneous pot conditions. After recovery period, no significant improvement in fluorescence dynamics of WT was observed although transgenic plants recovered from deformation. Spider plot of WT plants show significant deviation in dynamics under cold, heat, osmotic and salt stress conditions while transgenic lines did not show much deviation (Fig H in S1 File). Photosynthesis efficiency is maximal photochemical quantum yield of plants is represented in pictorial form as chlorophyll fluorescence imaging during control and different stress conditions for WT and transgenic line (Fig 4). The false color code depicted on the right side of images ranges from 0 (black) to 1 (pink) Level of significance is considered as P≤0.05. These results suggested that transgenic plants are easily adaptive to stress conditions as their fluorescence dynamics did not change significantly.


Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Chlorophyll fluorescence imaging (Fv/Fm) of WT and transgenic line (L1) was performed during control and stress conditions.Fv/Fm of non-treated WT and transgenic was carried out after 14 days of germination. Fv/Fm was carried out for WT and transgenic line after they were exposed to 37°C and 0°C for 4 h and again after10 and 15d recovery from heat and cold stress, respectively. For salt and different HM stress WT and transgenic line were watered with nutrient media containing NaCl (300 mM), 100 μM As(III), 200 μM As(V), 200 μM Cd and 400 μM Cr(VI) for two weeks and then Fv/Fm were measured. For osmotic stress WT and transgenic line were withheld from water for 14 d, and then rewatered for 7 d before Fv/Fm was measured. The false color code depicted on the right side of images ranges from 0 (black) to 1 (pink) Level of significance is considered as P≤0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581635&req=5

pone.0138574.g004: Chlorophyll fluorescence imaging (Fv/Fm) of WT and transgenic line (L1) was performed during control and stress conditions.Fv/Fm of non-treated WT and transgenic was carried out after 14 days of germination. Fv/Fm was carried out for WT and transgenic line after they were exposed to 37°C and 0°C for 4 h and again after10 and 15d recovery from heat and cold stress, respectively. For salt and different HM stress WT and transgenic line were watered with nutrient media containing NaCl (300 mM), 100 μM As(III), 200 μM As(V), 200 μM Cd and 400 μM Cr(VI) for two weeks and then Fv/Fm were measured. For osmotic stress WT and transgenic line were withheld from water for 14 d, and then rewatered for 7 d before Fv/Fm was measured. The false color code depicted on the right side of images ranges from 0 (black) to 1 (pink) Level of significance is considered as P≤0.05.
Mentions: Fluorescence dynamics for cold and heat stress were performed immediately after WT and transgenic lines were kept at 0°C and 37°C for 4 h and then after 10 d and 15 d of recovery under simultaneous pot conditions. After recovery period, no significant improvement in fluorescence dynamics of WT was observed although transgenic plants recovered from deformation. Spider plot of WT plants show significant deviation in dynamics under cold, heat, osmotic and salt stress conditions while transgenic lines did not show much deviation (Fig H in S1 File). Photosynthesis efficiency is maximal photochemical quantum yield of plants is represented in pictorial form as chlorophyll fluorescence imaging during control and different stress conditions for WT and transgenic line (Fig 4). The false color code depicted on the right side of images ranges from 0 (black) to 1 (pink) Level of significance is considered as P≤0.05. These results suggested that transgenic plants are easily adaptive to stress conditions as their fluorescence dynamics did not change significantly.

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus