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Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus

Expression of Os08g01480 in Arabidopsis confers tolerance to temperature stress.(A) Relative root length of the WT (Col-0) and transgenic plants after vertical growth of 11 d on ½ MS media plates after exposure at -20°C and 37°C for 4h in comparison to WT and transgenic lines grown in non treated media. (B) Phenotypic changes of WT and transgenic lines grown after exposure at -20°C and 37°C All values are the mean of triplicates (±SD) Values marked with similar letters are not significantly (Duncan’s test: p<0.05) different.
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pone.0138574.g003: Expression of Os08g01480 in Arabidopsis confers tolerance to temperature stress.(A) Relative root length of the WT (Col-0) and transgenic plants after vertical growth of 11 d on ½ MS media plates after exposure at -20°C and 37°C for 4h in comparison to WT and transgenic lines grown in non treated media. (B) Phenotypic changes of WT and transgenic lines grown after exposure at -20°C and 37°C All values are the mean of triplicates (±SD) Values marked with similar letters are not significantly (Duncan’s test: p<0.05) different.

Mentions: For studying effect of cold and heat, after stratification seeds were kept at -20°C and 37°C respectively for 4 h and then transferred to control conditions. There was ~60% and 40% decrease in root length of WT during cold and heat stress, respectively in comparison to non treated WT. On the other hand only ~25% and ~15% decrease of root length was recorded in transgenic lines at time of heat and cold stress, respectively when compared to NT transgenic plants. This result indicated ~40% and ~20% reduction in root length of WT compared to transgenic lines subjected to cold and heat stresses (Fig 3). We also observed increased lateral roots in transgenic lines especially in Line 1 and 3 (as expression level of Os08g01480 is higher in these lines) under different abiotic stress (Figs 1–3). These results indicate that Os08g01480 promotes lateral root development in transgenic lines which is an adoptive mechanism to various stress conditions. All these studies suggest that Arabidopsis expressing Os08g01480 exhibit tolerance against different environmental stresses.


Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Expression of Os08g01480 in Arabidopsis confers tolerance to temperature stress.(A) Relative root length of the WT (Col-0) and transgenic plants after vertical growth of 11 d on ½ MS media plates after exposure at -20°C and 37°C for 4h in comparison to WT and transgenic lines grown in non treated media. (B) Phenotypic changes of WT and transgenic lines grown after exposure at -20°C and 37°C All values are the mean of triplicates (±SD) Values marked with similar letters are not significantly (Duncan’s test: p<0.05) different.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581635&req=5

pone.0138574.g003: Expression of Os08g01480 in Arabidopsis confers tolerance to temperature stress.(A) Relative root length of the WT (Col-0) and transgenic plants after vertical growth of 11 d on ½ MS media plates after exposure at -20°C and 37°C for 4h in comparison to WT and transgenic lines grown in non treated media. (B) Phenotypic changes of WT and transgenic lines grown after exposure at -20°C and 37°C All values are the mean of triplicates (±SD) Values marked with similar letters are not significantly (Duncan’s test: p<0.05) different.
Mentions: For studying effect of cold and heat, after stratification seeds were kept at -20°C and 37°C respectively for 4 h and then transferred to control conditions. There was ~60% and 40% decrease in root length of WT during cold and heat stress, respectively in comparison to non treated WT. On the other hand only ~25% and ~15% decrease of root length was recorded in transgenic lines at time of heat and cold stress, respectively when compared to NT transgenic plants. This result indicated ~40% and ~20% reduction in root length of WT compared to transgenic lines subjected to cold and heat stresses (Fig 3). We also observed increased lateral roots in transgenic lines especially in Line 1 and 3 (as expression level of Os08g01480 is higher in these lines) under different abiotic stress (Figs 1–3). These results indicate that Os08g01480 promotes lateral root development in transgenic lines which is an adoptive mechanism to various stress conditions. All these studies suggest that Arabidopsis expressing Os08g01480 exhibit tolerance against different environmental stresses.

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus