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Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus

Expression of Os08g01480 in Arabidopsis confers tolerance for salt and osmotic stress.(A) Relative root length of the WT (Col-0) and transgenic plants after vertical growth of 11 d on ½ MS media plates supplemented with NaCl and Mannitol in comparison to WT and transgenic lines grown in non treated media. All values are the mean of triplicates (±SD) Values marked with similar letters are not significantly (Duncan’s test: p<0.05) different. (B) Phenotypic changes of WT and transgenic lines grown in ½ MS medium supplemented with NaCl and Mannitol.
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pone.0138574.g002: Expression of Os08g01480 in Arabidopsis confers tolerance for salt and osmotic stress.(A) Relative root length of the WT (Col-0) and transgenic plants after vertical growth of 11 d on ½ MS media plates supplemented with NaCl and Mannitol in comparison to WT and transgenic lines grown in non treated media. All values are the mean of triplicates (±SD) Values marked with similar letters are not significantly (Duncan’s test: p<0.05) different. (B) Phenotypic changes of WT and transgenic lines grown in ½ MS medium supplemented with NaCl and Mannitol.

Mentions: For salt and osmotic stress, WT and transgenic line were grown in NaCl (50 mM) and mannitol (150 mM) for 11 d. Root length of WT showed significant decrease (70%) as compared to transgenic line (30%-50%) during salt stress as compared to NT; WT and transgenic seedlings, whereas in osmotic stress, ~50% and up to 40% decrease was observed in WT and transgenic lines compared to NT; WT and transgenic plants. It is concluded that in WT there was ~40% and 20% more reduction in case of salt stress and osmotic stress respectively (Fig 2).


Expression of Rice CYP450-Like Gene (Os08g01480) in Arabidopsis Modulates Regulatory Network Leading to Heavy Metal and Other Abiotic Stress Tolerance.

Rai A, Singh R, Shirke PA, Tripathi RD, Trivedi PK, Chakrabarty D - PLoS ONE (2015)

Expression of Os08g01480 in Arabidopsis confers tolerance for salt and osmotic stress.(A) Relative root length of the WT (Col-0) and transgenic plants after vertical growth of 11 d on ½ MS media plates supplemented with NaCl and Mannitol in comparison to WT and transgenic lines grown in non treated media. All values are the mean of triplicates (±SD) Values marked with similar letters are not significantly (Duncan’s test: p<0.05) different. (B) Phenotypic changes of WT and transgenic lines grown in ½ MS medium supplemented with NaCl and Mannitol.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581635&req=5

pone.0138574.g002: Expression of Os08g01480 in Arabidopsis confers tolerance for salt and osmotic stress.(A) Relative root length of the WT (Col-0) and transgenic plants after vertical growth of 11 d on ½ MS media plates supplemented with NaCl and Mannitol in comparison to WT and transgenic lines grown in non treated media. All values are the mean of triplicates (±SD) Values marked with similar letters are not significantly (Duncan’s test: p<0.05) different. (B) Phenotypic changes of WT and transgenic lines grown in ½ MS medium supplemented with NaCl and Mannitol.
Mentions: For salt and osmotic stress, WT and transgenic line were grown in NaCl (50 mM) and mannitol (150 mM) for 11 d. Root length of WT showed significant decrease (70%) as compared to transgenic line (30%-50%) during salt stress as compared to NT; WT and transgenic seedlings, whereas in osmotic stress, ~50% and up to 40% decrease was observed in WT and transgenic lines compared to NT; WT and transgenic plants. It is concluded that in WT there was ~40% and 20% more reduction in case of salt stress and osmotic stress respectively (Fig 2).

Bottom Line: Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses.Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses.These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

View Article: PubMed Central - PubMed

Affiliation: Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110 001, India.

ABSTRACT
Heavy metal (HM) toxicity has become a grave problem in the world since it leads to hazardous effects on living organisms. Transcriptomic/proteomic studies in plants have identified a large number of metal-responsive gene families. Of these, cytochrome-P450 (CYPs) family members are composed of enzymes carrying out detoxification of exogenous molecules. Here, we report a CYP-like protein encoded by Os08g01480 locus in rice that helps the plant to combat HM and other abiotic stresses. To functionally characterize CYP-like gene, cDNA and promoter were isolated from rice to develop Arabidopsis transgenic lines. Heterologous expression of Os08g01480 in Arabidopsis provided significant tolerance towards abiotic stresses. In silico analysis reveals that Os08g01480 might help plants to combat environmental stress via modulating auxin metabolism. Transgenic lines expressing reporter gene under control of Os08g01480 promoter demonstrated differential promoter activity in different tissues during environmental stresses. These studies indicated that differential expression of Os08g01480 might be modulating response of plants towards environmental stresses as well as in different developmental stages.

No MeSH data available.


Related in: MedlinePlus