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Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.

Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V - PLoS ONE (2015)

Bottom Line: NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant.Pb-exposure significantly decreased in vivo NO level.The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.

ABSTRACT
Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

No MeSH data available.


Related in: MedlinePlus

NO production in wheat roots after 8 h of treatment with Pb and SNP. Root samples were stained for NO with DAF-2DA and were investigated under fluorescence microscope. (a) Control, (b) 100 μM SNP, (c) 50 μM Pb, (d) 50 μM Pb+ SNP, (e) 250 μM Pb, and (f) 250 μM Pb+ SNP.
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pone.0138713.g006: NO production in wheat roots after 8 h of treatment with Pb and SNP. Root samples were stained for NO with DAF-2DA and were investigated under fluorescence microscope. (a) Control, (b) 100 μM SNP, (c) 50 μM Pb, (d) 50 μM Pb+ SNP, (e) 250 μM Pb, and (f) 250 μM Pb+ SNP.

Mentions: To explore whether Pb-induced oxidative damage was associated with any alteration in the level of NO, intracellular nitrite (NO2−) content was measured. It was observed that compared to control, Pb-exposure significantly (p<0.05) decreased NO level in wheat roots in a concentration-dependent manner over 2–8 h of exposure period (Table 2). After 4 h, NO levels decreased by nearly 4% and 33% at 50 and 250 μM Pb exposure, respectively, over the control. The intracellular nitrite content declined further and ~15% and 44% decrease was noticed after 8 h of exposure to 50 and 250 μM Pb, respectively, as compared to the control. However, after SNP supplementation, there was a significant increase in intracellular nitrite content in all the treatments including control (Table 2). Enhanced NO generation upon SNP supplementation was confirmed by in situ NO detection using DAF-2DA dye, wherein roots from Pb+SNP treatments exhibited greater fluorescence (Fig 6).


Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.

Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V - PLoS ONE (2015)

NO production in wheat roots after 8 h of treatment with Pb and SNP. Root samples were stained for NO with DAF-2DA and were investigated under fluorescence microscope. (a) Control, (b) 100 μM SNP, (c) 50 μM Pb, (d) 50 μM Pb+ SNP, (e) 250 μM Pb, and (f) 250 μM Pb+ SNP.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581626&req=5

pone.0138713.g006: NO production in wheat roots after 8 h of treatment with Pb and SNP. Root samples were stained for NO with DAF-2DA and were investigated under fluorescence microscope. (a) Control, (b) 100 μM SNP, (c) 50 μM Pb, (d) 50 μM Pb+ SNP, (e) 250 μM Pb, and (f) 250 μM Pb+ SNP.
Mentions: To explore whether Pb-induced oxidative damage was associated with any alteration in the level of NO, intracellular nitrite (NO2−) content was measured. It was observed that compared to control, Pb-exposure significantly (p<0.05) decreased NO level in wheat roots in a concentration-dependent manner over 2–8 h of exposure period (Table 2). After 4 h, NO levels decreased by nearly 4% and 33% at 50 and 250 μM Pb exposure, respectively, over the control. The intracellular nitrite content declined further and ~15% and 44% decrease was noticed after 8 h of exposure to 50 and 250 μM Pb, respectively, as compared to the control. However, after SNP supplementation, there was a significant increase in intracellular nitrite content in all the treatments including control (Table 2). Enhanced NO generation upon SNP supplementation was confirmed by in situ NO detection using DAF-2DA dye, wherein roots from Pb+SNP treatments exhibited greater fluorescence (Fig 6).

Bottom Line: NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant.Pb-exposure significantly decreased in vivo NO level.The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.

ABSTRACT
Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

No MeSH data available.


Related in: MedlinePlus