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Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.

Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V - PLoS ONE (2015)

Bottom Line: NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant.Pb-exposure significantly decreased in vivo NO level.The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.

ABSTRACT
Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

No MeSH data available.


Related in: MedlinePlus

In situ histochemical localization showing the effect of SNP (100 μM) on Pb-induced loss of membrane integrity in wheat roots, after 2, 4, 6 and 8 h of Pb treatment.At each time period, roots inlcude: control (a), 100 μM SNP (b),50 μM Pb (c), 50 μM Pb+SNP (d), 250 μM Pb (e) and 250 μM Pb+SNP (f).
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pone.0138713.g005: In situ histochemical localization showing the effect of SNP (100 μM) on Pb-induced loss of membrane integrity in wheat roots, after 2, 4, 6 and 8 h of Pb treatment.At each time period, roots inlcude: control (a), 100 μM SNP (b),50 μM Pb (c), 50 μM Pb+SNP (d), 250 μM Pb (e) and 250 μM Pb+SNP (f).

Mentions: REL increased by ~24–49% and 48–83% at 50 and 250 μM Pb-exposure, respectively, over that in the control, from 2–8 h (Fig 4b). SNP supplementation significantly ameliorated Pb-toxicity during initial hours, i.e. up to 6 h in 50 μM Pb and up to 2 h in 250 μM Pb-treatment (Fig 4b). The ameliorating effect of SNP on membrane damage was further confirmed by in vivo staining of roots with Evans blue. Roots from Pb+SNP treatments stained less than those from Pb-alone treatments indicating an ameliorating / protective effect of SNP (Fig 5).


Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.

Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V - PLoS ONE (2015)

In situ histochemical localization showing the effect of SNP (100 μM) on Pb-induced loss of membrane integrity in wheat roots, after 2, 4, 6 and 8 h of Pb treatment.At each time period, roots inlcude: control (a), 100 μM SNP (b),50 μM Pb (c), 50 μM Pb+SNP (d), 250 μM Pb (e) and 250 μM Pb+SNP (f).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581626&req=5

pone.0138713.g005: In situ histochemical localization showing the effect of SNP (100 μM) on Pb-induced loss of membrane integrity in wheat roots, after 2, 4, 6 and 8 h of Pb treatment.At each time period, roots inlcude: control (a), 100 μM SNP (b),50 μM Pb (c), 50 μM Pb+SNP (d), 250 μM Pb (e) and 250 μM Pb+SNP (f).
Mentions: REL increased by ~24–49% and 48–83% at 50 and 250 μM Pb-exposure, respectively, over that in the control, from 2–8 h (Fig 4b). SNP supplementation significantly ameliorated Pb-toxicity during initial hours, i.e. up to 6 h in 50 μM Pb and up to 2 h in 250 μM Pb-treatment (Fig 4b). The ameliorating effect of SNP on membrane damage was further confirmed by in vivo staining of roots with Evans blue. Roots from Pb+SNP treatments stained less than those from Pb-alone treatments indicating an ameliorating / protective effect of SNP (Fig 5).

Bottom Line: NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant.Pb-exposure significantly decreased in vivo NO level.The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.

ABSTRACT
Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

No MeSH data available.


Related in: MedlinePlus