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Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.

Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V - PLoS ONE (2015)

Bottom Line: NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant.Pb-exposure significantly decreased in vivo NO level.The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.

ABSTRACT
Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

No MeSH data available.


Related in: MedlinePlus

Effect of SNP (100 μM) on the Pb-induced changes in the (a) concentration of hydrogen peroxide (H2O2, nM g–1 FW) and (b) relative electrolyte leakage (REL, %) of wheat seedlings determined before and after 2, 4, 6 and 8 h of exposure to Pb (50 and 250 μM, as lead nitrate).Data presented as mean±SE. * and ** represent significant difference of various treatments at 2, 4, 6 and 8 h stage from 0 h stage at p<0.05 and at p<0.01, respectively, applying post hoc Dunnett’s test. Different letters within a particular stage (2, 4, 6 or 8 h) represent significant difference at p<0.05 applying post hoc Tukey’s test.
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pone.0138713.g004: Effect of SNP (100 μM) on the Pb-induced changes in the (a) concentration of hydrogen peroxide (H2O2, nM g–1 FW) and (b) relative electrolyte leakage (REL, %) of wheat seedlings determined before and after 2, 4, 6 and 8 h of exposure to Pb (50 and 250 μM, as lead nitrate).Data presented as mean±SE. * and ** represent significant difference of various treatments at 2, 4, 6 and 8 h stage from 0 h stage at p<0.05 and at p<0.01, respectively, applying post hoc Dunnett’s test. Different letters within a particular stage (2, 4, 6 or 8 h) represent significant difference at p<0.05 applying post hoc Tukey’s test.

Mentions: ●HO content was enhanced by 205–253% and 470–507% during 2–8 h of exposure to 50 and 250 μM Pb, respectively, over that in the control. However, SNP reduced ●HO generation after 8 h by 10% and 7% in 50 and 250 μM Pb+SNP treatment, respectively, over Pb-alone treatments (Fig 2b). Likewise, H2O2 content increased by ~2.73–4.87 times and 3.73–6.06 times at 50 and 250 μM Pb-exposure, respectively, over that in the control, during 2–8 h of treatment (Fig 4a). Further, SNP supplementation ameliorated Pb (250 μM)-induced H2O2 accumulation by ~21.43% and 3.29% after 2 and 8 h of exposure, respectively as compared to Pb alone.


Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.

Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V - PLoS ONE (2015)

Effect of SNP (100 μM) on the Pb-induced changes in the (a) concentration of hydrogen peroxide (H2O2, nM g–1 FW) and (b) relative electrolyte leakage (REL, %) of wheat seedlings determined before and after 2, 4, 6 and 8 h of exposure to Pb (50 and 250 μM, as lead nitrate).Data presented as mean±SE. * and ** represent significant difference of various treatments at 2, 4, 6 and 8 h stage from 0 h stage at p<0.05 and at p<0.01, respectively, applying post hoc Dunnett’s test. Different letters within a particular stage (2, 4, 6 or 8 h) represent significant difference at p<0.05 applying post hoc Tukey’s test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581626&req=5

pone.0138713.g004: Effect of SNP (100 μM) on the Pb-induced changes in the (a) concentration of hydrogen peroxide (H2O2, nM g–1 FW) and (b) relative electrolyte leakage (REL, %) of wheat seedlings determined before and after 2, 4, 6 and 8 h of exposure to Pb (50 and 250 μM, as lead nitrate).Data presented as mean±SE. * and ** represent significant difference of various treatments at 2, 4, 6 and 8 h stage from 0 h stage at p<0.05 and at p<0.01, respectively, applying post hoc Dunnett’s test. Different letters within a particular stage (2, 4, 6 or 8 h) represent significant difference at p<0.05 applying post hoc Tukey’s test.
Mentions: ●HO content was enhanced by 205–253% and 470–507% during 2–8 h of exposure to 50 and 250 μM Pb, respectively, over that in the control. However, SNP reduced ●HO generation after 8 h by 10% and 7% in 50 and 250 μM Pb+SNP treatment, respectively, over Pb-alone treatments (Fig 2b). Likewise, H2O2 content increased by ~2.73–4.87 times and 3.73–6.06 times at 50 and 250 μM Pb-exposure, respectively, over that in the control, during 2–8 h of treatment (Fig 4a). Further, SNP supplementation ameliorated Pb (250 μM)-induced H2O2 accumulation by ~21.43% and 3.29% after 2 and 8 h of exposure, respectively as compared to Pb alone.

Bottom Line: NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant.Pb-exposure significantly decreased in vivo NO level.The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.

ABSTRACT
Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

No MeSH data available.


Related in: MedlinePlus