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Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.

Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V - PLoS ONE (2015)

Bottom Line: NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant.Pb-exposure significantly decreased in vivo NO level.The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.

ABSTRACT
Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

No MeSH data available.


Related in: MedlinePlus

Effect of SNP (100 μM) on the Pb-induced changes in the content of (a) superoxide anion (O2●−, μM g–1 FW), and (b) hydroxyl ion (●HO, nM g–1 FW) of wheat seedlings determined before and after 2, 4, 6 and 8 h of exposure to Pb (50 and 250 μM, as lead nitrate).Data presented as mean±SE. * and ** represent significant difference of various treatments at 2, 4, 6 and 8 h stage from 0 h stage at p<0.05 and at p<0.01, respectively, applying post hoc Dunnett’s test. Different letters within a particular stage (2, 4, 6 or 8 h) represent significant difference at p<0.05 applying post hoc Tukey’s test.
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pone.0138713.g002: Effect of SNP (100 μM) on the Pb-induced changes in the content of (a) superoxide anion (O2●−, μM g–1 FW), and (b) hydroxyl ion (●HO, nM g–1 FW) of wheat seedlings determined before and after 2, 4, 6 and 8 h of exposure to Pb (50 and 250 μM, as lead nitrate).Data presented as mean±SE. * and ** represent significant difference of various treatments at 2, 4, 6 and 8 h stage from 0 h stage at p<0.05 and at p<0.01, respectively, applying post hoc Dunnett’s test. Different letters within a particular stage (2, 4, 6 or 8 h) represent significant difference at p<0.05 applying post hoc Tukey’s test.

Mentions: O2●− content increased by ~ 80% and 206% over the untreated control in response to 50 and 250 μM Pb, respectively, after 8 h of treatment (Fig 2a). However, SNP supplementation reduced O2●− content by ~10% and 6.5% at 8 h compared to those treated with 50 μM and 250 μM Pb, respectively (Fig 2a).


Exogenous Nitric Oxide (NO) Interferes with Lead (Pb)-Induced Toxicity by Detoxifying Reactive Oxygen Species in Hydroponically Grown Wheat (Triticum aestivum) Roots.

Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V - PLoS ONE (2015)

Effect of SNP (100 μM) on the Pb-induced changes in the content of (a) superoxide anion (O2●−, μM g–1 FW), and (b) hydroxyl ion (●HO, nM g–1 FW) of wheat seedlings determined before and after 2, 4, 6 and 8 h of exposure to Pb (50 and 250 μM, as lead nitrate).Data presented as mean±SE. * and ** represent significant difference of various treatments at 2, 4, 6 and 8 h stage from 0 h stage at p<0.05 and at p<0.01, respectively, applying post hoc Dunnett’s test. Different letters within a particular stage (2, 4, 6 or 8 h) represent significant difference at p<0.05 applying post hoc Tukey’s test.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4581626&req=5

pone.0138713.g002: Effect of SNP (100 μM) on the Pb-induced changes in the content of (a) superoxide anion (O2●−, μM g–1 FW), and (b) hydroxyl ion (●HO, nM g–1 FW) of wheat seedlings determined before and after 2, 4, 6 and 8 h of exposure to Pb (50 and 250 μM, as lead nitrate).Data presented as mean±SE. * and ** represent significant difference of various treatments at 2, 4, 6 and 8 h stage from 0 h stage at p<0.05 and at p<0.01, respectively, applying post hoc Dunnett’s test. Different letters within a particular stage (2, 4, 6 or 8 h) represent significant difference at p<0.05 applying post hoc Tukey’s test.
Mentions: O2●− content increased by ~ 80% and 206% over the untreated control in response to 50 and 250 μM Pb, respectively, after 8 h of treatment (Fig 2a). However, SNP supplementation reduced O2●− content by ~10% and 6.5% at 8 h compared to those treated with 50 μM and 250 μM Pb, respectively (Fig 2a).

Bottom Line: NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant.Pb-exposure significantly decreased in vivo NO level.The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Environment Studies, Panjab University, Chandigarh, 160 014, India.

ABSTRACT
Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 μM) alone and in combination with SNP (100 μM) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure.

No MeSH data available.


Related in: MedlinePlus