Limits...
Reproducibility of Retinol Binding Protein 4 and Omentin-1 Measurements over a Four Months Period: A Reliability Study in a Cohort of 207 Apparently Healthy Participants.

Wittenbecher C, di Giuseppe R, Biemann R, Menzel J, Arregui M, Hoffmann J, Aleksandrova K, Boeing H, Isermann B, Schulze MB, Weikert C - PLoS ONE (2015)

Bottom Line: The present study aimed to assess the amount of biological variation of these two adipokines within individuals.The study sample comprised 207 participants (124 women and 83 men) from Potsdam (Germany) and surrounding areas, with an average age of 56.5 years (SD 4.2).Blood samples were collected from each participant twice, approximately four months apart.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; German Center for Diabetes Research (DZD), Neuherberg, Germany.

ABSTRACT
The reliability of single time point measurements of the novel adipokines retinol-binding protein 4 and omentin-1 in the blood has not been evaluated in large samples yet. The present study aimed to assess the amount of biological variation of these two adipokines within individuals. The study sample comprised 207 participants (124 women and 83 men) from Potsdam (Germany) and surrounding areas, with an average age of 56.5 years (SD 4.2). Blood samples were collected from each participant twice, approximately four months apart. Using enzyme linked immunosorbent assays, the concentrations of retinol-binding protein 4 and omentin-1 were determined in EDTA plasma. As indicators of reliability, intraclass correlation coefficients (ICCs) were calculated from the repeated biomarker measurements. The ICCs for repeated retinol-binding protein 4 and omentin-1 measurements were 0.77 (95% CI 0.71, 0.82) and 0.83 (95% CI 0.78, 0.87), respectively, indicating for both adipokines excellent reliability. ICCs were stable across strata according to sex, age, BMI, and blood pressure. Thus, for epidemiological studies it seems reasonable to rely on concentrations of retinol-binding protein 4 and omentin-1 in samples from a single time point if repeated measurements are not available.

No MeSH data available.


Related in: MedlinePlus

Bland-Altman-Plot.Agreement of repeated biomarker measurements in individuals in relation to the overall variability of measurements; agreement was calculated as difference in plasma-concentrations between the two blood-sampling occasions (t2-t1) within individuals; range of agreement was defined as mean ± 1.96 SD and is marked by the dashed lines. A: retinol-binding protein 4 (RBP4); B: omentin-1.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581620&req=5

pone.0138480.g001: Bland-Altman-Plot.Agreement of repeated biomarker measurements in individuals in relation to the overall variability of measurements; agreement was calculated as difference in plasma-concentrations between the two blood-sampling occasions (t2-t1) within individuals; range of agreement was defined as mean ± 1.96 SD and is marked by the dashed lines. A: retinol-binding protein 4 (RBP4); B: omentin-1.

Mentions: The agreement between repeated adipokine measurements within individuals in relation to individual means was visualized in Bland-Altman-Plots (Fig 1). The Bland-Altman-Plots do not show clustering of differences at certain ranges of the adipokine concentrations.


Reproducibility of Retinol Binding Protein 4 and Omentin-1 Measurements over a Four Months Period: A Reliability Study in a Cohort of 207 Apparently Healthy Participants.

Wittenbecher C, di Giuseppe R, Biemann R, Menzel J, Arregui M, Hoffmann J, Aleksandrova K, Boeing H, Isermann B, Schulze MB, Weikert C - PLoS ONE (2015)

Bland-Altman-Plot.Agreement of repeated biomarker measurements in individuals in relation to the overall variability of measurements; agreement was calculated as difference in plasma-concentrations between the two blood-sampling occasions (t2-t1) within individuals; range of agreement was defined as mean ± 1.96 SD and is marked by the dashed lines. A: retinol-binding protein 4 (RBP4); B: omentin-1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581620&req=5

pone.0138480.g001: Bland-Altman-Plot.Agreement of repeated biomarker measurements in individuals in relation to the overall variability of measurements; agreement was calculated as difference in plasma-concentrations between the two blood-sampling occasions (t2-t1) within individuals; range of agreement was defined as mean ± 1.96 SD and is marked by the dashed lines. A: retinol-binding protein 4 (RBP4); B: omentin-1.
Mentions: The agreement between repeated adipokine measurements within individuals in relation to individual means was visualized in Bland-Altman-Plots (Fig 1). The Bland-Altman-Plots do not show clustering of differences at certain ranges of the adipokine concentrations.

Bottom Line: The present study aimed to assess the amount of biological variation of these two adipokines within individuals.The study sample comprised 207 participants (124 women and 83 men) from Potsdam (Germany) and surrounding areas, with an average age of 56.5 years (SD 4.2).Blood samples were collected from each participant twice, approximately four months apart.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; German Center for Diabetes Research (DZD), Neuherberg, Germany.

ABSTRACT
The reliability of single time point measurements of the novel adipokines retinol-binding protein 4 and omentin-1 in the blood has not been evaluated in large samples yet. The present study aimed to assess the amount of biological variation of these two adipokines within individuals. The study sample comprised 207 participants (124 women and 83 men) from Potsdam (Germany) and surrounding areas, with an average age of 56.5 years (SD 4.2). Blood samples were collected from each participant twice, approximately four months apart. Using enzyme linked immunosorbent assays, the concentrations of retinol-binding protein 4 and omentin-1 were determined in EDTA plasma. As indicators of reliability, intraclass correlation coefficients (ICCs) were calculated from the repeated biomarker measurements. The ICCs for repeated retinol-binding protein 4 and omentin-1 measurements were 0.77 (95% CI 0.71, 0.82) and 0.83 (95% CI 0.78, 0.87), respectively, indicating for both adipokines excellent reliability. ICCs were stable across strata according to sex, age, BMI, and blood pressure. Thus, for epidemiological studies it seems reasonable to rely on concentrations of retinol-binding protein 4 and omentin-1 in samples from a single time point if repeated measurements are not available.

No MeSH data available.


Related in: MedlinePlus