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Differences of IL-1β Receptors Expression by Immunocompetent Cells Subsets in Rheumatoid Arthritis.

Alshevskaya AA, Lopatnikova JA, Shkaruba NS, Chumasova OA, Sizikov AE, Karaulov AV, Kozlov VA, Sennikov SV - Mediators Inflamm. (2015)

Bottom Line: The effectiveness of the cytokine depends not only on the percentage of receptor-positive cells in an immunocompetent subset but also on the density of receptor expression.The objective of this study was to investigate the expression of IL-1β membrane-bound receptors (IL-1R1 and IL-1R2) in terms of the percentage of receptor-positive cells and the number of receptors per cell in different subsets of immune cells in RA patients before and after a course of basic (excluding anticytokine) therapy and in healthy individuals.The importance of determining both the relative percentage of cells expressing receptors to immunomodulatory cytokines and the number of membrane-bound receptors per cell is highlighted by evidence of unidirectional or multidirectional changing of these parameters according to cell subset and health status.

View Article: PubMed Central - PubMed

Affiliation: Federal State Budgetary Scientific Institution "Research Institute of Fundamental and Clinical Immunology", Yadrintsevskaya Street 14, Novosibirsk 630099, Russia.

ABSTRACT
IL-1β is involved in the induction and maintenance of chronic inflammation in rheumatoid arthritis (RA). Its activity is regulated and induced by soluble and membrane-bound receptors, respectively. The effectiveness of the cytokine depends not only on the percentage of receptor-positive cells in an immunocompetent subset but also on the density of receptor expression. The objective of this study was to investigate the expression of IL-1β membrane-bound receptors (IL-1R1 and IL-1R2) in terms of the percentage of receptor-positive cells and the number of receptors per cell in different subsets of immune cells in RA patients before and after a course of basic (excluding anticytokine) therapy and in healthy individuals. The resulting data indicate differences in the expression of IL-1β receptors among T cells, B cells, and monocytes in healthy volunteers and in rheumatoid arthritis patients. The importance of determining both the relative percentage of cells expressing receptors to immunomodulatory cytokines and the number of membrane-bound receptors per cell is highlighted by evidence of unidirectional or multidirectional changing of these parameters according to cell subset and health status.

No MeSH data available.


Related in: MedlinePlus

(a) Serum level of IL-1β in healthy individuals (n = 150) and RA patients in the acute phase (n = 28) and after response to treatment (n = 25), in pg/mL. The data are presented as the mean ± standard deviation of the mean. The arrows denote significance of intergroup differences, p < 0.05.
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fig5: (a) Serum level of IL-1β in healthy individuals (n = 150) and RA patients in the acute phase (n = 28) and after response to treatment (n = 25), in pg/mL. The data are presented as the mean ± standard deviation of the mean. The arrows denote significance of intergroup differences, p < 0.05.

Mentions: Because the effect of IL-1 on cells depends significantly on the relative serum concentration of soluble IL-1β receptors and cytokine, the serum contents of IL-1β (Figure 5) and soluble IL-1β receptors types 1 and 2 (Figures 6 and 7) were studied by ELISA in RA patients in the acute phase and RA patients who responded to treatment.


Differences of IL-1β Receptors Expression by Immunocompetent Cells Subsets in Rheumatoid Arthritis.

Alshevskaya AA, Lopatnikova JA, Shkaruba NS, Chumasova OA, Sizikov AE, Karaulov AV, Kozlov VA, Sennikov SV - Mediators Inflamm. (2015)

(a) Serum level of IL-1β in healthy individuals (n = 150) and RA patients in the acute phase (n = 28) and after response to treatment (n = 25), in pg/mL. The data are presented as the mean ± standard deviation of the mean. The arrows denote significance of intergroup differences, p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4581579&req=5

fig5: (a) Serum level of IL-1β in healthy individuals (n = 150) and RA patients in the acute phase (n = 28) and after response to treatment (n = 25), in pg/mL. The data are presented as the mean ± standard deviation of the mean. The arrows denote significance of intergroup differences, p < 0.05.
Mentions: Because the effect of IL-1 on cells depends significantly on the relative serum concentration of soluble IL-1β receptors and cytokine, the serum contents of IL-1β (Figure 5) and soluble IL-1β receptors types 1 and 2 (Figures 6 and 7) were studied by ELISA in RA patients in the acute phase and RA patients who responded to treatment.

Bottom Line: The effectiveness of the cytokine depends not only on the percentage of receptor-positive cells in an immunocompetent subset but also on the density of receptor expression.The objective of this study was to investigate the expression of IL-1β membrane-bound receptors (IL-1R1 and IL-1R2) in terms of the percentage of receptor-positive cells and the number of receptors per cell in different subsets of immune cells in RA patients before and after a course of basic (excluding anticytokine) therapy and in healthy individuals.The importance of determining both the relative percentage of cells expressing receptors to immunomodulatory cytokines and the number of membrane-bound receptors per cell is highlighted by evidence of unidirectional or multidirectional changing of these parameters according to cell subset and health status.

View Article: PubMed Central - PubMed

Affiliation: Federal State Budgetary Scientific Institution "Research Institute of Fundamental and Clinical Immunology", Yadrintsevskaya Street 14, Novosibirsk 630099, Russia.

ABSTRACT
IL-1β is involved in the induction and maintenance of chronic inflammation in rheumatoid arthritis (RA). Its activity is regulated and induced by soluble and membrane-bound receptors, respectively. The effectiveness of the cytokine depends not only on the percentage of receptor-positive cells in an immunocompetent subset but also on the density of receptor expression. The objective of this study was to investigate the expression of IL-1β membrane-bound receptors (IL-1R1 and IL-1R2) in terms of the percentage of receptor-positive cells and the number of receptors per cell in different subsets of immune cells in RA patients before and after a course of basic (excluding anticytokine) therapy and in healthy individuals. The resulting data indicate differences in the expression of IL-1β receptors among T cells, B cells, and monocytes in healthy volunteers and in rheumatoid arthritis patients. The importance of determining both the relative percentage of cells expressing receptors to immunomodulatory cytokines and the number of membrane-bound receptors per cell is highlighted by evidence of unidirectional or multidirectional changing of these parameters according to cell subset and health status.

No MeSH data available.


Related in: MedlinePlus