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Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement.

Zhang J, Li G, Li H, Pu X, Jiang J, Chai L, Zheng B, Cui C, Yang Z, Zhu Y, Jiang L - Int J Genomics (2015)

Bottom Line: A total of 40,320 nonredundant unigenes were identified using B. rapa (AA genome) and B. oleracea (CC genome) as reference genomes.A total of 6,816 differentially expressed genes (DEGs) were mapped in the A and C genomes with 4,946 DEGs displayed nonadditively by comparing the gene expression patterns among the three samples.The present study could be helpful for the better understanding of the determination and regulation of mechanisms of heterosis to aid Brassica improvement.

View Article: PubMed Central - PubMed

Affiliation: Crop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, China.

ABSTRACT
The hybrid between Brassica napus and B. rapa displays obvious heterosis in both growth performance and stress tolerances. A comparative transcriptome analysis for B. napus (A(n)A(n)CC genome), B. rapa (A(r)A(r) genome), and its hybrid F1 (A(n)A(r)C genome) was carried out to reveal the possible molecular mechanisms of heterosis at the gene expression level. A total of 40,320 nonredundant unigenes were identified using B. rapa (AA genome) and B. oleracea (CC genome) as reference genomes. A total of 6,816 differentially expressed genes (DEGs) were mapped in the A and C genomes with 4,946 DEGs displayed nonadditively by comparing the gene expression patterns among the three samples. The coexistence of nonadditive DEGs including high-parent dominance, low-parent dominance, overdominance, and underdominance was observed in the gene action modes of F1 hybrid, which were potentially related to the heterosis. The coexistence of multiple gene actions in the hybrid was observed and provided a list of candidate genes and pathways for heterosis. The expression bias of transposable element-associated genes was also observed in the hybrid compared to their parents. The present study could be helpful for the better understanding of the determination and regulation of mechanisms of heterosis to aid Brassica improvement.

No MeSH data available.


Related in: MedlinePlus

Seed germination (a) and 2-week-old seedlings (b) of P1, P2, and F1 germplasm.
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fig2: Seed germination (a) and 2-week-old seedlings (b) of P1, P2, and F1 germplasm.

Mentions: The hybridization of B. napus cv. CWH-2 (P1) and B. rapa ssp. chinensis cv. Qianjin (P2) was made in 2010-2011 oilseed growing season. Root-tip chromosome assessments of F1 showed that all F1 plants had a chromosome number of 29 (Figure 1), suggesting that they were hybrids. The seeds' sizes of F1 hybrids were intermediate to those of the parents, but the seed color resembled that of B. napus (Figure 2(a)). Root growth of the F1 over the first 24 h was similar to P1. Leaf morphology of the F1 was more similar to B. rapa than to B. napus (Figure 2). In 4-week-old seedlings, the F1 plant displayed stronger roots than either of the parents (Figure 2(b)) while adult F1 plants displayed the similar heading time to B. napus.


Transcriptome Analysis of Interspecific Hybrid between Brassica napus and B. rapa Reveals Heterosis for Oil Rape Improvement.

Zhang J, Li G, Li H, Pu X, Jiang J, Chai L, Zheng B, Cui C, Yang Z, Zhu Y, Jiang L - Int J Genomics (2015)

Seed germination (a) and 2-week-old seedlings (b) of P1, P2, and F1 germplasm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4581553&req=5

fig2: Seed germination (a) and 2-week-old seedlings (b) of P1, P2, and F1 germplasm.
Mentions: The hybridization of B. napus cv. CWH-2 (P1) and B. rapa ssp. chinensis cv. Qianjin (P2) was made in 2010-2011 oilseed growing season. Root-tip chromosome assessments of F1 showed that all F1 plants had a chromosome number of 29 (Figure 1), suggesting that they were hybrids. The seeds' sizes of F1 hybrids were intermediate to those of the parents, but the seed color resembled that of B. napus (Figure 2(a)). Root growth of the F1 over the first 24 h was similar to P1. Leaf morphology of the F1 was more similar to B. rapa than to B. napus (Figure 2). In 4-week-old seedlings, the F1 plant displayed stronger roots than either of the parents (Figure 2(b)) while adult F1 plants displayed the similar heading time to B. napus.

Bottom Line: A total of 40,320 nonredundant unigenes were identified using B. rapa (AA genome) and B. oleracea (CC genome) as reference genomes.A total of 6,816 differentially expressed genes (DEGs) were mapped in the A and C genomes with 4,946 DEGs displayed nonadditively by comparing the gene expression patterns among the three samples.The present study could be helpful for the better understanding of the determination and regulation of mechanisms of heterosis to aid Brassica improvement.

View Article: PubMed Central - PubMed

Affiliation: Crop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6110066, China.

ABSTRACT
The hybrid between Brassica napus and B. rapa displays obvious heterosis in both growth performance and stress tolerances. A comparative transcriptome analysis for B. napus (A(n)A(n)CC genome), B. rapa (A(r)A(r) genome), and its hybrid F1 (A(n)A(r)C genome) was carried out to reveal the possible molecular mechanisms of heterosis at the gene expression level. A total of 40,320 nonredundant unigenes were identified using B. rapa (AA genome) and B. oleracea (CC genome) as reference genomes. A total of 6,816 differentially expressed genes (DEGs) were mapped in the A and C genomes with 4,946 DEGs displayed nonadditively by comparing the gene expression patterns among the three samples. The coexistence of nonadditive DEGs including high-parent dominance, low-parent dominance, overdominance, and underdominance was observed in the gene action modes of F1 hybrid, which were potentially related to the heterosis. The coexistence of multiple gene actions in the hybrid was observed and provided a list of candidate genes and pathways for heterosis. The expression bias of transposable element-associated genes was also observed in the hybrid compared to their parents. The present study could be helpful for the better understanding of the determination and regulation of mechanisms of heterosis to aid Brassica improvement.

No MeSH data available.


Related in: MedlinePlus