Limits...
Electroacupuncture at Zusanli Prevents Severe Scalds-Induced Gut Ischemia and Paralysis by Activating the Cholinergic Pathway.

Wang H, Wang L, Shi X, Qi S, Hu S, Tong Z, Ma Z, Qian Y, Litscher D, Litscher G - Evid Based Complement Alternat Med (2015)

Bottom Line: Severe burn injuries may result in gastrointestinal paralysis, and barrier dysfunction due to gut ischemia and lowered vagus excitability.The plasma diamine oxidase (DAO) and intestinal permeability decreased significantly after scald injury in the EA group compared with others.However, EA after atropine injection or cervical vagotomy failed to improve intestinal motility and mucosa blood flow suggesting that the mechanism of EA may be related to the activation of the cholinergic nerve pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of TCM and Acupuncture, Chinese PLA General Hospital, Beijing 100853, China.

ABSTRACT
Severe burn injuries may result in gastrointestinal paralysis, and barrier dysfunction due to gut ischemia and lowered vagus excitability. In this study we investigate whether electroacupuncture (EA) at Zusanli (ST36) could prevent severe scalds-induced gut ischemia, paralysis, and barrier dysfunction and whether the protective role of EA at ST36 is related to the vagus nerve. 35% burn area rats were divided into six groups: (a) EAN: EA nonchannel acupoints followed by scald injury; (b) EA: EA at ST36 after scald injury; (c) VGX/EA: vagotomy (VGX) before EA at ST36 and scald injury; (d) VGX/EAN: VGX before EAN and scald injury; (e) atropine/EA: applying atropine before scald injury and then EA at ST36; (f) atropine/EAN: applying atropine before scald injury and then EA at nonchannel acupoints. EA at the Zusanli point significantly promoted the intestinal impelling ratio and increased the amount of mucosal blood flow after scald injury. The plasma diamine oxidase (DAO) and intestinal permeability decreased significantly after scald injury in the EA group compared with others. However, EA after atropine injection or cervical vagotomy failed to improve intestinal motility and mucosa blood flow suggesting that the mechanism of EA may be related to the activation of the cholinergic nerve pathway.

No MeSH data available.


Related in: MedlinePlus

The intestinal permeability was detected in an in vivo assay 6 h after the scalds injury. Data are expressed as means ± SD (n = five animals at every time point per group). # versus EA group, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4581501&req=5

fig3: The intestinal permeability was detected in an in vivo assay 6 h after the scalds injury. Data are expressed as means ± SD (n = five animals at every time point per group). # versus EA group, P < 0.05.

Mentions: The intestinal permeability was evaluated in an in vivo assay using FITC-dextran 6 h after the scald (Figure 3). Animals in the EA group had a significantly lower level of plasma FITC-dextran when compared with the EAN group (574.31 ng/mL ± 149.45 ng/mL versus 1671.26 ng/mL ± 315.13 ng/mL, P < 0.05). However, when abdominal vagotomy or atropine injection was performed before EA at ST36, the intestinal permeability was indistinguishable from animals in the EAN group, and animals in the VGX/EAN or atropine/EAN group also had no protection against reducing intestinal permeability compared with animals in the EAN group (1542.36 ng/mL ± 370.61 ng/mL and 1645.87 ng/mL ± 312.33 ng/mL versus 1671.26 ng/mL ± 315.13 ng/mL). These data indicate that EA at ST36 can only offer protection to the gut in the presence of intact neurenteric innervation and may exert its protective effects via the cholinergic nerve pathway.


Electroacupuncture at Zusanli Prevents Severe Scalds-Induced Gut Ischemia and Paralysis by Activating the Cholinergic Pathway.

Wang H, Wang L, Shi X, Qi S, Hu S, Tong Z, Ma Z, Qian Y, Litscher D, Litscher G - Evid Based Complement Alternat Med (2015)

The intestinal permeability was detected in an in vivo assay 6 h after the scalds injury. Data are expressed as means ± SD (n = five animals at every time point per group). # versus EA group, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4581501&req=5

fig3: The intestinal permeability was detected in an in vivo assay 6 h after the scalds injury. Data are expressed as means ± SD (n = five animals at every time point per group). # versus EA group, P < 0.05.
Mentions: The intestinal permeability was evaluated in an in vivo assay using FITC-dextran 6 h after the scald (Figure 3). Animals in the EA group had a significantly lower level of plasma FITC-dextran when compared with the EAN group (574.31 ng/mL ± 149.45 ng/mL versus 1671.26 ng/mL ± 315.13 ng/mL, P < 0.05). However, when abdominal vagotomy or atropine injection was performed before EA at ST36, the intestinal permeability was indistinguishable from animals in the EAN group, and animals in the VGX/EAN or atropine/EAN group also had no protection against reducing intestinal permeability compared with animals in the EAN group (1542.36 ng/mL ± 370.61 ng/mL and 1645.87 ng/mL ± 312.33 ng/mL versus 1671.26 ng/mL ± 315.13 ng/mL). These data indicate that EA at ST36 can only offer protection to the gut in the presence of intact neurenteric innervation and may exert its protective effects via the cholinergic nerve pathway.

Bottom Line: Severe burn injuries may result in gastrointestinal paralysis, and barrier dysfunction due to gut ischemia and lowered vagus excitability.The plasma diamine oxidase (DAO) and intestinal permeability decreased significantly after scald injury in the EA group compared with others.However, EA after atropine injection or cervical vagotomy failed to improve intestinal motility and mucosa blood flow suggesting that the mechanism of EA may be related to the activation of the cholinergic nerve pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of TCM and Acupuncture, Chinese PLA General Hospital, Beijing 100853, China.

ABSTRACT
Severe burn injuries may result in gastrointestinal paralysis, and barrier dysfunction due to gut ischemia and lowered vagus excitability. In this study we investigate whether electroacupuncture (EA) at Zusanli (ST36) could prevent severe scalds-induced gut ischemia, paralysis, and barrier dysfunction and whether the protective role of EA at ST36 is related to the vagus nerve. 35% burn area rats were divided into six groups: (a) EAN: EA nonchannel acupoints followed by scald injury; (b) EA: EA at ST36 after scald injury; (c) VGX/EA: vagotomy (VGX) before EA at ST36 and scald injury; (d) VGX/EAN: VGX before EAN and scald injury; (e) atropine/EA: applying atropine before scald injury and then EA at ST36; (f) atropine/EAN: applying atropine before scald injury and then EA at nonchannel acupoints. EA at the Zusanli point significantly promoted the intestinal impelling ratio and increased the amount of mucosal blood flow after scald injury. The plasma diamine oxidase (DAO) and intestinal permeability decreased significantly after scald injury in the EA group compared with others. However, EA after atropine injection or cervical vagotomy failed to improve intestinal motility and mucosa blood flow suggesting that the mechanism of EA may be related to the activation of the cholinergic nerve pathway.

No MeSH data available.


Related in: MedlinePlus