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MicroRNAs in urine are not biomarkers of multiple myeloma.

Sedlaříková L, Bešše L, Novosadová S, Kubaczková V, Radová L, Staník M, Krejčí M, Hájek R, Ševčíková S - J Negat Results Biomed (2015)

Bottom Line: We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them.Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.

View Article: PubMed Central - PubMed

Affiliation: Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. lena.sedlarikova@gmail.com.

ABSTRACT

Background: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.

Results: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.

Conclusions: Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.

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Related in: MedlinePlus

Hierarchical clustering of differentially expressed miRNAs from urine between MM patients (yellow) and HD (blue) using heatmaps. Different expression is expressed by range of red (overexpression) vs green (low expression) colors
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Fig1: Hierarchical clustering of differentially expressed miRNAs from urine between MM patients (yellow) and HD (blue) using heatmaps. Different expression is expressed by range of red (overexpression) vs green (low expression) colors

Mentions: Screening of miRNA in urine revealed 20 deregulated miRNAs (p < 0.05 for all miRNAs) between MM and HD (Fig. 1). For further validation, we chose 8 most deregulated miRNAs between MM and HD: miR-200c-3p, miR-29b-3p, miR-29c-3p, miR-22-3p, miR-29a-3p, miR-25-3p, miR-106b-5p, miR-18a-3p (Table 1). Expression levels of these miRNAs were further verified on a larger cohort of MM patients, RCC patients and HD. Results from validation revealed that expression levels of chosen miRNAs from urine of MM patients are not statistically different (p = 0.05) from HD and RCC patients (Fig. 2). Results from validation did not confirm statistical significance from the screening. Only differences in expression levels of miR-22-3p between MM patients and HD in urine (p = 0.090) remained close to statistical significance (Table 2).Fig. 1


MicroRNAs in urine are not biomarkers of multiple myeloma.

Sedlaříková L, Bešše L, Novosadová S, Kubaczková V, Radová L, Staník M, Krejčí M, Hájek R, Ševčíková S - J Negat Results Biomed (2015)

Hierarchical clustering of differentially expressed miRNAs from urine between MM patients (yellow) and HD (blue) using heatmaps. Different expression is expressed by range of red (overexpression) vs green (low expression) colors
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4581458&req=5

Fig1: Hierarchical clustering of differentially expressed miRNAs from urine between MM patients (yellow) and HD (blue) using heatmaps. Different expression is expressed by range of red (overexpression) vs green (low expression) colors
Mentions: Screening of miRNA in urine revealed 20 deregulated miRNAs (p < 0.05 for all miRNAs) between MM and HD (Fig. 1). For further validation, we chose 8 most deregulated miRNAs between MM and HD: miR-200c-3p, miR-29b-3p, miR-29c-3p, miR-22-3p, miR-29a-3p, miR-25-3p, miR-106b-5p, miR-18a-3p (Table 1). Expression levels of these miRNAs were further verified on a larger cohort of MM patients, RCC patients and HD. Results from validation revealed that expression levels of chosen miRNAs from urine of MM patients are not statistically different (p = 0.05) from HD and RCC patients (Fig. 2). Results from validation did not confirm statistical significance from the screening. Only differences in expression levels of miR-22-3p between MM patients and HD in urine (p = 0.090) remained close to statistical significance (Table 2).Fig. 1

Bottom Line: We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them.Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.

View Article: PubMed Central - PubMed

Affiliation: Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. lena.sedlarikova@gmail.com.

ABSTRACT

Background: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.

Results: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.

Conclusions: Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.

Show MeSH
Related in: MedlinePlus