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Lipopolysaccharide Effect on Vascular Endothelial Factor and Matrix Metalloproteinases in Leukemic Cell Lines In vitro.

Hajighasemi F, Gheini MH - Iran J Cancer Prev (2015)

Bottom Line: U937 cells have produced a large amount of VEGF without any stimulus and LPS has not shown any substantial effect on VEGF production by these cells.However THP1 cells have produced a small amount of VEGF without stimulation and LPS significantly has increased VEGF production in these cells dose-dependently.Our results have shown that LPS might be a potential inducer/enhancer of VEGF production and MMP-2/MMP-9 activity (angiogenic factors) in leukemia.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Shahed University, Tehran, IR Iran.

ABSTRACT

Background: Angiogenesis, the process of new vessels generation, plays a critical role in tumor invasion and metastasis. Vascular Endothelial Growth Factor (VEGF), as a cytokine, and Matrix Metalloproteinases (MMPs), has been the important factors that involved in angiogenesis. Lipopolysaccharide (LPS) has an essential effect on angiogenesis.

Objectives: In this study the effect of LPS on VEGF production and MMP-2/MMP-9 activity in two leukemic cell lines has been assessed in vitro.

Materials and methods: Human leukemic U937 and THP1 cells were cultured in complete RPMI medium. Then the cells at the exponential growth phase were incubated with different concentrations of LPS (0 - 4 μg/mL) for 48 hours. Then the level of VEGF production and MMP-2/MMP-9 activity in cell culture supernatants were evaluated with the ELISA standard kits and gelatin zymography respectively.

Results: U937 cells have produced a large amount of VEGF without any stimulus and LPS has not shown any substantial effect on VEGF production by these cells. However THP1 cells have produced a small amount of VEGF without stimulation and LPS significantly has increased VEGF production in these cells dose-dependently. Moreover LPS significantly has augmented the MMP-2/MMP-9 activity in the both leukemic cell lines in a dose-dependent manner.

Conclusions: Our results have shown that LPS might be a potential inducer/enhancer of VEGF production and MMP-2/MMP-9 activity (angiogenic factors) in leukemia. Moreover the LPS effect on angiogenesis might be in part, due to its stimulatory effects on VEGF and MMPs. Overall LPS-stimulated leukemic cells might be good models for study and planning the useful therapeutic approaches for angiogenesis- dependent diseases.

No MeSH data available.


Related in: MedlinePlus

Effect of LPS on MMP-9 Activity in U937 and THP1 Leukemic CellsThe THP1 leukemic cells (1 × 106 cells/mL) were cultured in complete RPMI-1640 medium and then were stimulated with different concentrations of lypopolysaccharide (LPS) (0 - 4 µg/mL) for 48 hours. At the end of treatment, MMP-9 activity in conditioned medium was measured by gelatin zymography. Data are Mean ± SEM of three independent experiments. *P < 0.05 was considered significant.
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A2327FIG3: Effect of LPS on MMP-9 Activity in U937 and THP1 Leukemic CellsThe THP1 leukemic cells (1 × 106 cells/mL) were cultured in complete RPMI-1640 medium and then were stimulated with different concentrations of lypopolysaccharide (LPS) (0 - 4 µg/mL) for 48 hours. At the end of treatment, MMP-9 activity in conditioned medium was measured by gelatin zymography. Data are Mean ± SEM of three independent experiments. *P < 0.05 was considered significant.

Mentions: When U937 cells cultured alone with no stimulus, faint bands related to MMP-9 activity have detected. LPS stimulation, significantly increased MMP-9 activity in U937 cells compared with untreated control cells. The LPS -increased MMP-9 activity in U937 cells was dose-dependent as illustrated in Table 3 and Figure 3 (P < 0.05).


Lipopolysaccharide Effect on Vascular Endothelial Factor and Matrix Metalloproteinases in Leukemic Cell Lines In vitro.

Hajighasemi F, Gheini MH - Iran J Cancer Prev (2015)

Effect of LPS on MMP-9 Activity in U937 and THP1 Leukemic CellsThe THP1 leukemic cells (1 × 106 cells/mL) were cultured in complete RPMI-1640 medium and then were stimulated with different concentrations of lypopolysaccharide (LPS) (0 - 4 µg/mL) for 48 hours. At the end of treatment, MMP-9 activity in conditioned medium was measured by gelatin zymography. Data are Mean ± SEM of three independent experiments. *P < 0.05 was considered significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581372&req=5

A2327FIG3: Effect of LPS on MMP-9 Activity in U937 and THP1 Leukemic CellsThe THP1 leukemic cells (1 × 106 cells/mL) were cultured in complete RPMI-1640 medium and then were stimulated with different concentrations of lypopolysaccharide (LPS) (0 - 4 µg/mL) for 48 hours. At the end of treatment, MMP-9 activity in conditioned medium was measured by gelatin zymography. Data are Mean ± SEM of three independent experiments. *P < 0.05 was considered significant.
Mentions: When U937 cells cultured alone with no stimulus, faint bands related to MMP-9 activity have detected. LPS stimulation, significantly increased MMP-9 activity in U937 cells compared with untreated control cells. The LPS -increased MMP-9 activity in U937 cells was dose-dependent as illustrated in Table 3 and Figure 3 (P < 0.05).

Bottom Line: U937 cells have produced a large amount of VEGF without any stimulus and LPS has not shown any substantial effect on VEGF production by these cells.However THP1 cells have produced a small amount of VEGF without stimulation and LPS significantly has increased VEGF production in these cells dose-dependently.Our results have shown that LPS might be a potential inducer/enhancer of VEGF production and MMP-2/MMP-9 activity (angiogenic factors) in leukemia.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Shahed University, Tehran, IR Iran.

ABSTRACT

Background: Angiogenesis, the process of new vessels generation, plays a critical role in tumor invasion and metastasis. Vascular Endothelial Growth Factor (VEGF), as a cytokine, and Matrix Metalloproteinases (MMPs), has been the important factors that involved in angiogenesis. Lipopolysaccharide (LPS) has an essential effect on angiogenesis.

Objectives: In this study the effect of LPS on VEGF production and MMP-2/MMP-9 activity in two leukemic cell lines has been assessed in vitro.

Materials and methods: Human leukemic U937 and THP1 cells were cultured in complete RPMI medium. Then the cells at the exponential growth phase were incubated with different concentrations of LPS (0 - 4 μg/mL) for 48 hours. Then the level of VEGF production and MMP-2/MMP-9 activity in cell culture supernatants were evaluated with the ELISA standard kits and gelatin zymography respectively.

Results: U937 cells have produced a large amount of VEGF without any stimulus and LPS has not shown any substantial effect on VEGF production by these cells. However THP1 cells have produced a small amount of VEGF without stimulation and LPS significantly has increased VEGF production in these cells dose-dependently. Moreover LPS significantly has augmented the MMP-2/MMP-9 activity in the both leukemic cell lines in a dose-dependent manner.

Conclusions: Our results have shown that LPS might be a potential inducer/enhancer of VEGF production and MMP-2/MMP-9 activity (angiogenic factors) in leukemia. Moreover the LPS effect on angiogenesis might be in part, due to its stimulatory effects on VEGF and MMPs. Overall LPS-stimulated leukemic cells might be good models for study and planning the useful therapeutic approaches for angiogenesis- dependent diseases.

No MeSH data available.


Related in: MedlinePlus