Limits...
Lipopolysaccharide Effect on Vascular Endothelial Factor and Matrix Metalloproteinases in Leukemic Cell Lines In vitro.

Hajighasemi F, Gheini MH - Iran J Cancer Prev (2015)

Bottom Line: U937 cells have produced a large amount of VEGF without any stimulus and LPS has not shown any substantial effect on VEGF production by these cells.However THP1 cells have produced a small amount of VEGF without stimulation and LPS significantly has increased VEGF production in these cells dose-dependently.Our results have shown that LPS might be a potential inducer/enhancer of VEGF production and MMP-2/MMP-9 activity (angiogenic factors) in leukemia.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Shahed University, Tehran, IR Iran.

ABSTRACT

Background: Angiogenesis, the process of new vessels generation, plays a critical role in tumor invasion and metastasis. Vascular Endothelial Growth Factor (VEGF), as a cytokine, and Matrix Metalloproteinases (MMPs), has been the important factors that involved in angiogenesis. Lipopolysaccharide (LPS) has an essential effect on angiogenesis.

Objectives: In this study the effect of LPS on VEGF production and MMP-2/MMP-9 activity in two leukemic cell lines has been assessed in vitro.

Materials and methods: Human leukemic U937 and THP1 cells were cultured in complete RPMI medium. Then the cells at the exponential growth phase were incubated with different concentrations of LPS (0 - 4 μg/mL) for 48 hours. Then the level of VEGF production and MMP-2/MMP-9 activity in cell culture supernatants were evaluated with the ELISA standard kits and gelatin zymography respectively.

Results: U937 cells have produced a large amount of VEGF without any stimulus and LPS has not shown any substantial effect on VEGF production by these cells. However THP1 cells have produced a small amount of VEGF without stimulation and LPS significantly has increased VEGF production in these cells dose-dependently. Moreover LPS significantly has augmented the MMP-2/MMP-9 activity in the both leukemic cell lines in a dose-dependent manner.

Conclusions: Our results have shown that LPS might be a potential inducer/enhancer of VEGF production and MMP-2/MMP-9 activity (angiogenic factors) in leukemia. Moreover the LPS effect on angiogenesis might be in part, due to its stimulatory effects on VEGF and MMPs. Overall LPS-stimulated leukemic cells might be good models for study and planning the useful therapeutic approaches for angiogenesis- dependent diseases.

No MeSH data available.


Related in: MedlinePlus

Effect of LPS on VEGF Production by U937 AND THP1 Leukemic CellsThe leukemic cells (1 × 106 cells/mL) were cultured in complete RPMI-1640 medium and then were stimulated with different concentrations of lypopolysaccharide (LPS) (0 - 4 µg/mL) for 48 hours. At the end of incubation, VEGF concentration in conditioned medium was quantified by ELISA. Data are Mean ± SEM of three independent experiments.*P < 0.05 was considered significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581372&req=5

A2327FIG1: Effect of LPS on VEGF Production by U937 AND THP1 Leukemic CellsThe leukemic cells (1 × 106 cells/mL) were cultured in complete RPMI-1640 medium and then were stimulated with different concentrations of lypopolysaccharide (LPS) (0 - 4 µg/mL) for 48 hours. At the end of incubation, VEGF concentration in conditioned medium was quantified by ELISA. Data are Mean ± SEM of three independent experiments.*P < 0.05 was considered significant.

Mentions: Results depicted in Figure 1, demonstrate that U937 cells produced a large amount of VEGF without any stimulation and LPS did not show any significant effect on VEGF production by these cells.


Lipopolysaccharide Effect on Vascular Endothelial Factor and Matrix Metalloproteinases in Leukemic Cell Lines In vitro.

Hajighasemi F, Gheini MH - Iran J Cancer Prev (2015)

Effect of LPS on VEGF Production by U937 AND THP1 Leukemic CellsThe leukemic cells (1 × 106 cells/mL) were cultured in complete RPMI-1640 medium and then were stimulated with different concentrations of lypopolysaccharide (LPS) (0 - 4 µg/mL) for 48 hours. At the end of incubation, VEGF concentration in conditioned medium was quantified by ELISA. Data are Mean ± SEM of three independent experiments.*P < 0.05 was considered significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581372&req=5

A2327FIG1: Effect of LPS on VEGF Production by U937 AND THP1 Leukemic CellsThe leukemic cells (1 × 106 cells/mL) were cultured in complete RPMI-1640 medium and then were stimulated with different concentrations of lypopolysaccharide (LPS) (0 - 4 µg/mL) for 48 hours. At the end of incubation, VEGF concentration in conditioned medium was quantified by ELISA. Data are Mean ± SEM of three independent experiments.*P < 0.05 was considered significant.
Mentions: Results depicted in Figure 1, demonstrate that U937 cells produced a large amount of VEGF without any stimulation and LPS did not show any significant effect on VEGF production by these cells.

Bottom Line: U937 cells have produced a large amount of VEGF without any stimulus and LPS has not shown any substantial effect on VEGF production by these cells.However THP1 cells have produced a small amount of VEGF without stimulation and LPS significantly has increased VEGF production in these cells dose-dependently.Our results have shown that LPS might be a potential inducer/enhancer of VEGF production and MMP-2/MMP-9 activity (angiogenic factors) in leukemia.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Shahed University, Tehran, IR Iran.

ABSTRACT

Background: Angiogenesis, the process of new vessels generation, plays a critical role in tumor invasion and metastasis. Vascular Endothelial Growth Factor (VEGF), as a cytokine, and Matrix Metalloproteinases (MMPs), has been the important factors that involved in angiogenesis. Lipopolysaccharide (LPS) has an essential effect on angiogenesis.

Objectives: In this study the effect of LPS on VEGF production and MMP-2/MMP-9 activity in two leukemic cell lines has been assessed in vitro.

Materials and methods: Human leukemic U937 and THP1 cells were cultured in complete RPMI medium. Then the cells at the exponential growth phase were incubated with different concentrations of LPS (0 - 4 μg/mL) for 48 hours. Then the level of VEGF production and MMP-2/MMP-9 activity in cell culture supernatants were evaluated with the ELISA standard kits and gelatin zymography respectively.

Results: U937 cells have produced a large amount of VEGF without any stimulus and LPS has not shown any substantial effect on VEGF production by these cells. However THP1 cells have produced a small amount of VEGF without stimulation and LPS significantly has increased VEGF production in these cells dose-dependently. Moreover LPS significantly has augmented the MMP-2/MMP-9 activity in the both leukemic cell lines in a dose-dependent manner.

Conclusions: Our results have shown that LPS might be a potential inducer/enhancer of VEGF production and MMP-2/MMP-9 activity (angiogenic factors) in leukemia. Moreover the LPS effect on angiogenesis might be in part, due to its stimulatory effects on VEGF and MMPs. Overall LPS-stimulated leukemic cells might be good models for study and planning the useful therapeutic approaches for angiogenesis- dependent diseases.

No MeSH data available.


Related in: MedlinePlus