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17β-Estradiol Stimulates Generation of Reactive Species Oxygen and Nitric Oxide in Ovarian Adenocarcinoma Cells (OVCAR 3).

Maleki J, Nourbakhsh M, Shabani M, Korani M, Nourazarian SM, Ostadali Dahaghi MR, Moghadasi MH - Iran J Cancer Prev (2015)

Bottom Line: Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.Progesterone was also effective in reducing ROS and NO generation.NO and ROS are important molecules in signaling networks in cell.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Iran University of Medical Sciences, Tehran, IR Iran.

ABSTRACT

Background: Experimental and epidemiological evidence supports a role for steroid hormones in the pathogenesis of ovarian cancer. Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.

Objectives: In the present study, we investigated the effect of E2 on production of ROS and NO in ovarian cancer cells.

Materials and methods: Ovarian adenocarcinoma cell line (OVCAR-3) was cultured and treated with various concentrations of E2, antioxidants (N-acetyle cysteine and Ebselen) and ICI182780 as an estrogen receptor antagonist. MTT test was performed to evaluate cell viability. NO and ROS levels were measured by Griess and DCFH-DA methods, respectively.

Results: ROS levels as well as NO levels were increased in OVCAR-3 cells treated with E2. The increase in ROS production was in parallel with increased cell viability which indicates that estrogen-induced ROS can participate in cancer progression. ICI182780 abolished E2-induced ROS production. Progesterone was also effective in reducing ROS and NO generation.

Conclusions: NO and ROS are important molecules in signaling networks in cell. These molecules can be used as therapeutic targets for prevention and treatment of ovary cancer and other estrogen-induced malignancies.

No MeSH data available.


Related in: MedlinePlus

(A) E2 Induced ROS Production in OVCAR-3 Cells in a Dose Dependent MannerCells Were Seeded at 3 × 105 Cells Per Well. After Serum Starvation for 24 hours Cells Were Treated With Various Concentrations of E2 for 24 hours. (B) Cells Were Pre-Treated With 1.0 Mm NAC, Ph 7.0 or 40 µm Ebselen For 4 H Before DCF Assay. Cells Were Also Treated With E2 (100 Nm) Together With Progesterone (P4) (10 - 6 M). And ICI182780 (ICI) (10 - 5M). The Data Shown is the Mean ± SE From at Least Three Separate Experiments. *P < 0.05; **P < 0.01; *** P < 0.001.
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A2332FIG2: (A) E2 Induced ROS Production in OVCAR-3 Cells in a Dose Dependent MannerCells Were Seeded at 3 × 105 Cells Per Well. After Serum Starvation for 24 hours Cells Were Treated With Various Concentrations of E2 for 24 hours. (B) Cells Were Pre-Treated With 1.0 Mm NAC, Ph 7.0 or 40 µm Ebselen For 4 H Before DCF Assay. Cells Were Also Treated With E2 (100 Nm) Together With Progesterone (P4) (10 - 6 M). And ICI182780 (ICI) (10 - 5M). The Data Shown is the Mean ± SE From at Least Three Separate Experiments. *P < 0.05; **P < 0.01; *** P < 0.001.

Mentions: The ability of various concentrations of E2 on rate of reactive oxygen species (ROS) generation was investigated in our study. An increase in intracellular peroxide levels was detected in E2-treated cells, and the 100 nM dose of E2 induced the highest levels of ROS production (Figure 2).


17β-Estradiol Stimulates Generation of Reactive Species Oxygen and Nitric Oxide in Ovarian Adenocarcinoma Cells (OVCAR 3).

Maleki J, Nourbakhsh M, Shabani M, Korani M, Nourazarian SM, Ostadali Dahaghi MR, Moghadasi MH - Iran J Cancer Prev (2015)

(A) E2 Induced ROS Production in OVCAR-3 Cells in a Dose Dependent MannerCells Were Seeded at 3 × 105 Cells Per Well. After Serum Starvation for 24 hours Cells Were Treated With Various Concentrations of E2 for 24 hours. (B) Cells Were Pre-Treated With 1.0 Mm NAC, Ph 7.0 or 40 µm Ebselen For 4 H Before DCF Assay. Cells Were Also Treated With E2 (100 Nm) Together With Progesterone (P4) (10 - 6 M). And ICI182780 (ICI) (10 - 5M). The Data Shown is the Mean ± SE From at Least Three Separate Experiments. *P < 0.05; **P < 0.01; *** P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581366&req=5

A2332FIG2: (A) E2 Induced ROS Production in OVCAR-3 Cells in a Dose Dependent MannerCells Were Seeded at 3 × 105 Cells Per Well. After Serum Starvation for 24 hours Cells Were Treated With Various Concentrations of E2 for 24 hours. (B) Cells Were Pre-Treated With 1.0 Mm NAC, Ph 7.0 or 40 µm Ebselen For 4 H Before DCF Assay. Cells Were Also Treated With E2 (100 Nm) Together With Progesterone (P4) (10 - 6 M). And ICI182780 (ICI) (10 - 5M). The Data Shown is the Mean ± SE From at Least Three Separate Experiments. *P < 0.05; **P < 0.01; *** P < 0.001.
Mentions: The ability of various concentrations of E2 on rate of reactive oxygen species (ROS) generation was investigated in our study. An increase in intracellular peroxide levels was detected in E2-treated cells, and the 100 nM dose of E2 induced the highest levels of ROS production (Figure 2).

Bottom Line: Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.Progesterone was also effective in reducing ROS and NO generation.NO and ROS are important molecules in signaling networks in cell.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Iran University of Medical Sciences, Tehran, IR Iran.

ABSTRACT

Background: Experimental and epidemiological evidence supports a role for steroid hormones in the pathogenesis of ovarian cancer. Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.

Objectives: In the present study, we investigated the effect of E2 on production of ROS and NO in ovarian cancer cells.

Materials and methods: Ovarian adenocarcinoma cell line (OVCAR-3) was cultured and treated with various concentrations of E2, antioxidants (N-acetyle cysteine and Ebselen) and ICI182780 as an estrogen receptor antagonist. MTT test was performed to evaluate cell viability. NO and ROS levels were measured by Griess and DCFH-DA methods, respectively.

Results: ROS levels as well as NO levels were increased in OVCAR-3 cells treated with E2. The increase in ROS production was in parallel with increased cell viability which indicates that estrogen-induced ROS can participate in cancer progression. ICI182780 abolished E2-induced ROS production. Progesterone was also effective in reducing ROS and NO generation.

Conclusions: NO and ROS are important molecules in signaling networks in cell. These molecules can be used as therapeutic targets for prevention and treatment of ovary cancer and other estrogen-induced malignancies.

No MeSH data available.


Related in: MedlinePlus