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17β-Estradiol Stimulates Generation of Reactive Species Oxygen and Nitric Oxide in Ovarian Adenocarcinoma Cells (OVCAR 3).

Maleki J, Nourbakhsh M, Shabani M, Korani M, Nourazarian SM, Ostadali Dahaghi MR, Moghadasi MH - Iran J Cancer Prev (2015)

Bottom Line: Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.Progesterone was also effective in reducing ROS and NO generation.NO and ROS are important molecules in signaling networks in cell.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Iran University of Medical Sciences, Tehran, IR Iran.

ABSTRACT

Background: Experimental and epidemiological evidence supports a role for steroid hormones in the pathogenesis of ovarian cancer. Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.

Objectives: In the present study, we investigated the effect of E2 on production of ROS and NO in ovarian cancer cells.

Materials and methods: Ovarian adenocarcinoma cell line (OVCAR-3) was cultured and treated with various concentrations of E2, antioxidants (N-acetyle cysteine and Ebselen) and ICI182780 as an estrogen receptor antagonist. MTT test was performed to evaluate cell viability. NO and ROS levels were measured by Griess and DCFH-DA methods, respectively.

Results: ROS levels as well as NO levels were increased in OVCAR-3 cells treated with E2. The increase in ROS production was in parallel with increased cell viability which indicates that estrogen-induced ROS can participate in cancer progression. ICI182780 abolished E2-induced ROS production. Progesterone was also effective in reducing ROS and NO generation.

Conclusions: NO and ROS are important molecules in signaling networks in cell. These molecules can be used as therapeutic targets for prevention and treatment of ovary cancer and other estrogen-induced malignancies.

No MeSH data available.


Related in: MedlinePlus

17β-Estradiol Increased Cell Viability of Ovarian Cancer CellsOVCAR3 Cells Were Seeded in 96-Well Plates in Complete Medium. After 24 Hours Of Incubation With Phenol Red-Free Medium Supplemented With Dextran-Coated Charcoal-Stripped Serum, Cells Were Treated With The Indicated Concentrations of E2 or Vehicle Alone (C) For 48 Hours. MTT Assay Was Used to Assess Cell Viability. The Data Shown is The Mean ± SE From at Least Three Separate Experiments. *P < 0.05; **P < 0.01; ***P < 0.001.
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A2332FIG1: 17β-Estradiol Increased Cell Viability of Ovarian Cancer CellsOVCAR3 Cells Were Seeded in 96-Well Plates in Complete Medium. After 24 Hours Of Incubation With Phenol Red-Free Medium Supplemented With Dextran-Coated Charcoal-Stripped Serum, Cells Were Treated With The Indicated Concentrations of E2 or Vehicle Alone (C) For 48 Hours. MTT Assay Was Used to Assess Cell Viability. The Data Shown is The Mean ± SE From at Least Three Separate Experiments. *P < 0.05; **P < 0.01; ***P < 0.001.

Mentions: Human OVCAR-3 adenocarcinoma cells have been shown to express estrogen receptors. In this study, cell proliferation was significantly induced by E2 characterized by increased cell viability (Figure 1).


17β-Estradiol Stimulates Generation of Reactive Species Oxygen and Nitric Oxide in Ovarian Adenocarcinoma Cells (OVCAR 3).

Maleki J, Nourbakhsh M, Shabani M, Korani M, Nourazarian SM, Ostadali Dahaghi MR, Moghadasi MH - Iran J Cancer Prev (2015)

17β-Estradiol Increased Cell Viability of Ovarian Cancer CellsOVCAR3 Cells Were Seeded in 96-Well Plates in Complete Medium. After 24 Hours Of Incubation With Phenol Red-Free Medium Supplemented With Dextran-Coated Charcoal-Stripped Serum, Cells Were Treated With The Indicated Concentrations of E2 or Vehicle Alone (C) For 48 Hours. MTT Assay Was Used to Assess Cell Viability. The Data Shown is The Mean ± SE From at Least Three Separate Experiments. *P < 0.05; **P < 0.01; ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581366&req=5

A2332FIG1: 17β-Estradiol Increased Cell Viability of Ovarian Cancer CellsOVCAR3 Cells Were Seeded in 96-Well Plates in Complete Medium. After 24 Hours Of Incubation With Phenol Red-Free Medium Supplemented With Dextran-Coated Charcoal-Stripped Serum, Cells Were Treated With The Indicated Concentrations of E2 or Vehicle Alone (C) For 48 Hours. MTT Assay Was Used to Assess Cell Viability. The Data Shown is The Mean ± SE From at Least Three Separate Experiments. *P < 0.05; **P < 0.01; ***P < 0.001.
Mentions: Human OVCAR-3 adenocarcinoma cells have been shown to express estrogen receptors. In this study, cell proliferation was significantly induced by E2 characterized by increased cell viability (Figure 1).

Bottom Line: Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.Progesterone was also effective in reducing ROS and NO generation.NO and ROS are important molecules in signaling networks in cell.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Iran University of Medical Sciences, Tehran, IR Iran.

ABSTRACT

Background: Experimental and epidemiological evidence supports a role for steroid hormones in the pathogenesis of ovarian cancer. Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis.

Objectives: In the present study, we investigated the effect of E2 on production of ROS and NO in ovarian cancer cells.

Materials and methods: Ovarian adenocarcinoma cell line (OVCAR-3) was cultured and treated with various concentrations of E2, antioxidants (N-acetyle cysteine and Ebselen) and ICI182780 as an estrogen receptor antagonist. MTT test was performed to evaluate cell viability. NO and ROS levels were measured by Griess and DCFH-DA methods, respectively.

Results: ROS levels as well as NO levels were increased in OVCAR-3 cells treated with E2. The increase in ROS production was in parallel with increased cell viability which indicates that estrogen-induced ROS can participate in cancer progression. ICI182780 abolished E2-induced ROS production. Progesterone was also effective in reducing ROS and NO generation.

Conclusions: NO and ROS are important molecules in signaling networks in cell. These molecules can be used as therapeutic targets for prevention and treatment of ovary cancer and other estrogen-induced malignancies.

No MeSH data available.


Related in: MedlinePlus