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Development of a New Monomer for the Synthesis of Intrinsic Antimicrobial Polymers with Enhanced Material Properties.

Brodkorb F, Fischer B, Kalbfleisch K, Robers O, Braun C, Dohlen S, Kreyenschmidt J, Lorenz R, Kreyenschmidt M - Int J Mol Sci (2015)

Bottom Line: The use of biocidal compounds in polymers is steadily increasing because it is one solution to the need for safety and hygiene.The biocidal action results from contact of the polymer to the microorganisms, with no release of active molecules.The antimicrobial activity was analyzed by the test method JIS Z 2801:2000.

View Article: PubMed Central - PubMed

Affiliation: Institute of Construction and Functional Materials, University of Applied Sciences Münster, Stegerwaldstraße 39, 48565 Steinfurt, Germany. f.brodkorb@fh-muenster.de.

ABSTRACT
The use of biocidal compounds in polymers is steadily increasing because it is one solution to the need for safety and hygiene. It is possible to incorporate an antimicrobial moiety to a polymer. These polymers are referred to as intrinsic antimicrobial. The biocidal action results from contact of the polymer to the microorganisms, with no release of active molecules. This is particularly important in critical fields like food technology, medicine and ventilation technology, where migration or leaching is crucial and undesirable. The isomers N-(1,1-dimethylethyl)-4-ethenyl-benzenamine and N-(1,1-dimethyl-ethyl)-3-ethenyl-benzenamine (TBAMS) are novel (Co-)Monomers for intrinsic anti-microbial polymers. The secondary amines were prepared and polymerized to the corresponding water insoluble polymer. The antimicrobial activity was analyzed by the test method JIS Z 2801:2000. Investigations revealed a high antimicrobial activity against Staphylococcus aureus and Escherichia coli with a reduction level of >4.5 log10 units. Furthermore, scanning electron microscopy (SEM) of E. coli. in contact with the polymer indicates a bactericidal action which is caused by disruption of the bacteria cell membranes, leading to lysis of the cells.

No MeSH data available.


Related in: MedlinePlus

(A) E. coli after 12 h incubation in blank petri dish; (B) E. coli after 12 h incubation in petri dish coated with poly(TBAMS).
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ijms-16-20050-f008: (A) E. coli after 12 h incubation in blank petri dish; (B) E. coli after 12 h incubation in petri dish coated with poly(TBAMS).

Mentions: SEM was applied in order to investigate morphological changes of bacteria in contact with poly(TBAMS). In the case of the gram negative E. coli after 12 h of contact pronounced morphological changes were observed, whereas cells in the blank petri dishes remained unchanged. Furthermore the cells show a exudate of fibrous and granular material after incubation with poly(TBAMS). The surface of treated bacteria appears to be wrinkled and rough if compared with untreated cells (Figure 8). Additionally, a lot of debris was observed in the treated E. coli. Most likely the debris was formed due the lysis of cells.


Development of a New Monomer for the Synthesis of Intrinsic Antimicrobial Polymers with Enhanced Material Properties.

Brodkorb F, Fischer B, Kalbfleisch K, Robers O, Braun C, Dohlen S, Kreyenschmidt J, Lorenz R, Kreyenschmidt M - Int J Mol Sci (2015)

(A) E. coli after 12 h incubation in blank petri dish; (B) E. coli after 12 h incubation in petri dish coated with poly(TBAMS).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581340&req=5

ijms-16-20050-f008: (A) E. coli after 12 h incubation in blank petri dish; (B) E. coli after 12 h incubation in petri dish coated with poly(TBAMS).
Mentions: SEM was applied in order to investigate morphological changes of bacteria in contact with poly(TBAMS). In the case of the gram negative E. coli after 12 h of contact pronounced morphological changes were observed, whereas cells in the blank petri dishes remained unchanged. Furthermore the cells show a exudate of fibrous and granular material after incubation with poly(TBAMS). The surface of treated bacteria appears to be wrinkled and rough if compared with untreated cells (Figure 8). Additionally, a lot of debris was observed in the treated E. coli. Most likely the debris was formed due the lysis of cells.

Bottom Line: The use of biocidal compounds in polymers is steadily increasing because it is one solution to the need for safety and hygiene.The biocidal action results from contact of the polymer to the microorganisms, with no release of active molecules.The antimicrobial activity was analyzed by the test method JIS Z 2801:2000.

View Article: PubMed Central - PubMed

Affiliation: Institute of Construction and Functional Materials, University of Applied Sciences Münster, Stegerwaldstraße 39, 48565 Steinfurt, Germany. f.brodkorb@fh-muenster.de.

ABSTRACT
The use of biocidal compounds in polymers is steadily increasing because it is one solution to the need for safety and hygiene. It is possible to incorporate an antimicrobial moiety to a polymer. These polymers are referred to as intrinsic antimicrobial. The biocidal action results from contact of the polymer to the microorganisms, with no release of active molecules. This is particularly important in critical fields like food technology, medicine and ventilation technology, where migration or leaching is crucial and undesirable. The isomers N-(1,1-dimethylethyl)-4-ethenyl-benzenamine and N-(1,1-dimethyl-ethyl)-3-ethenyl-benzenamine (TBAMS) are novel (Co-)Monomers for intrinsic anti-microbial polymers. The secondary amines were prepared and polymerized to the corresponding water insoluble polymer. The antimicrobial activity was analyzed by the test method JIS Z 2801:2000. Investigations revealed a high antimicrobial activity against Staphylococcus aureus and Escherichia coli with a reduction level of >4.5 log10 units. Furthermore, scanning electron microscopy (SEM) of E. coli. in contact with the polymer indicates a bactericidal action which is caused by disruption of the bacteria cell membranes, leading to lysis of the cells.

No MeSH data available.


Related in: MedlinePlus