Limits...
Extraction of Peptidoglycan from L. paracasei subp. Paracasei X12 and Its Preliminary Mechanisms of Inducing Immunogenic Cell Death in HT-29 Cells.

Tian PJ, Li BL, Shan YJ, Zhang JN, Chen JY, Yu M, Zhang LW - Int J Mol Sci (2015)

Bottom Line: X12-PG could induce the production of apoptotic bodies observed by transmission electron microscopy (TEM).X12-PG could significantly induced the translocation of calreticulin (CRT) and the release of high mobility group box 1 protein (HMGB1), the two notable hallmarks of immunogenic cell death (ICD), with the endoplastic reticulum (ER) damaged and subsequently intracellular [Ca(2+)] elevated.Our findings implied that X12-PG could induce the ICD of HT-29 cells through targeting at the ER.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, No. 73 Huanghe Road, Harbin 150000, China. tianpei0202@gmail.com.

ABSTRACT
L. paracasei subp. paracasei X12 was previously isolated from a Chinese traditional fermented cheese with anticancer activities and probiotic potential. Herein, the integral peptidoglycan (X12-PG) was extracted by a modified trichloroacetic acid (TCA) method. X12-PG contained the four representative amino acids Asp, Glu, Ala and Lys, and displayed the similar lysozyme sensitivity, UV-visible scanning spectrum and molecular weight as the peptidoglycan standard. X12-PG could induce the production of apoptotic bodies observed by transmission electron microscopy (TEM). X12-PG could significantly induced the translocation of calreticulin (CRT) and the release of high mobility group box 1 protein (HMGB1), the two notable hallmarks of immunogenic cell death (ICD), with the endoplastic reticulum (ER) damaged and subsequently intracellular [Ca(2+)] elevated. Our findings implied that X12-PG could induce the ICD of HT-29 cells through targeting at the ER. The present results may enlighten the prospect of probiotics in the prevention of colon cancer.

No MeSH data available.


Related in: MedlinePlus

Structural features of the integral peptidoglycan isolated from L. paracasei subp. paracasei X12 (X12-PG) and the L. paracasei subp. paracasei X12 bacteria (X12) observed by transmission electron microscope (TEM). (A,B) The structure of X12 (×20,000, ×40,000); (C,D) The cylindrical structure of internal X12-PG (×20,000, ×40,000).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4581339&req=5

ijms-16-20033-f001: Structural features of the integral peptidoglycan isolated from L. paracasei subp. paracasei X12 (X12-PG) and the L. paracasei subp. paracasei X12 bacteria (X12) observed by transmission electron microscope (TEM). (A,B) The structure of X12 (×20,000, ×40,000); (C,D) The cylindrical structure of internal X12-PG (×20,000, ×40,000).

Mentions: Extraction of X12-PG was processed according to the modified trichloroacetic acid (TCA) method. To maintain the physical integrity of the cell wall structure, this isolation procedure did not result in any physical destructive processes. Also this extraction removed virtually all the teichoic acid from the cell wall. The extraction yield of X12-PG is 6.79%, which was calculated by the dry weight of extracts/bacterial sludge ratio. The results of electron microscopic observation are shown in Figure 1, where a cystic structure and extremely neat edges are seen in X12-PG. In sharp contrast, the X12 bacterial cells are more distinct with dark interior regions.


Extraction of Peptidoglycan from L. paracasei subp. Paracasei X12 and Its Preliminary Mechanisms of Inducing Immunogenic Cell Death in HT-29 Cells.

Tian PJ, Li BL, Shan YJ, Zhang JN, Chen JY, Yu M, Zhang LW - Int J Mol Sci (2015)

Structural features of the integral peptidoglycan isolated from L. paracasei subp. paracasei X12 (X12-PG) and the L. paracasei subp. paracasei X12 bacteria (X12) observed by transmission electron microscope (TEM). (A,B) The structure of X12 (×20,000, ×40,000); (C,D) The cylindrical structure of internal X12-PG (×20,000, ×40,000).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581339&req=5

ijms-16-20033-f001: Structural features of the integral peptidoglycan isolated from L. paracasei subp. paracasei X12 (X12-PG) and the L. paracasei subp. paracasei X12 bacteria (X12) observed by transmission electron microscope (TEM). (A,B) The structure of X12 (×20,000, ×40,000); (C,D) The cylindrical structure of internal X12-PG (×20,000, ×40,000).
Mentions: Extraction of X12-PG was processed according to the modified trichloroacetic acid (TCA) method. To maintain the physical integrity of the cell wall structure, this isolation procedure did not result in any physical destructive processes. Also this extraction removed virtually all the teichoic acid from the cell wall. The extraction yield of X12-PG is 6.79%, which was calculated by the dry weight of extracts/bacterial sludge ratio. The results of electron microscopic observation are shown in Figure 1, where a cystic structure and extremely neat edges are seen in X12-PG. In sharp contrast, the X12 bacterial cells are more distinct with dark interior regions.

Bottom Line: X12-PG could induce the production of apoptotic bodies observed by transmission electron microscopy (TEM).X12-PG could significantly induced the translocation of calreticulin (CRT) and the release of high mobility group box 1 protein (HMGB1), the two notable hallmarks of immunogenic cell death (ICD), with the endoplastic reticulum (ER) damaged and subsequently intracellular [Ca(2+)] elevated.Our findings implied that X12-PG could induce the ICD of HT-29 cells through targeting at the ER.

View Article: PubMed Central - PubMed

Affiliation: School of Food Science and Engineering, Harbin Institute of Technology, No. 73 Huanghe Road, Harbin 150000, China. tianpei0202@gmail.com.

ABSTRACT
L. paracasei subp. paracasei X12 was previously isolated from a Chinese traditional fermented cheese with anticancer activities and probiotic potential. Herein, the integral peptidoglycan (X12-PG) was extracted by a modified trichloroacetic acid (TCA) method. X12-PG contained the four representative amino acids Asp, Glu, Ala and Lys, and displayed the similar lysozyme sensitivity, UV-visible scanning spectrum and molecular weight as the peptidoglycan standard. X12-PG could induce the production of apoptotic bodies observed by transmission electron microscopy (TEM). X12-PG could significantly induced the translocation of calreticulin (CRT) and the release of high mobility group box 1 protein (HMGB1), the two notable hallmarks of immunogenic cell death (ICD), with the endoplastic reticulum (ER) damaged and subsequently intracellular [Ca(2+)] elevated. Our findings implied that X12-PG could induce the ICD of HT-29 cells through targeting at the ER. The present results may enlighten the prospect of probiotics in the prevention of colon cancer.

No MeSH data available.


Related in: MedlinePlus