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Exploiting Size-Dependent Drag and Magnetic Forces for Size-Specific Separation of Magnetic Nanoparticles.

Rogers HB, Anani T, Choi YS, Beyers RJ, David AE - Int J Mol Sci (2015)

Bottom Line: Magnetic field-flow fractionation, however, was found to be an effective method for the separation of polydisperse suspensions of iron oxide nanoparticles with diameters greater than 20 nm.Both transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis were used to confirm the size of the MNPs.Further development of this work could lead to MNPs with the narrow size distributions necessary for their in vitro and in vivo optimization.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, USA. hunterrogers2014@u.northwestern.edu.

ABSTRACT
Realizing the full potential of magnetic nanoparticles (MNPs) in nanomedicine requires the optimization of their physical and chemical properties. Elucidation of the effects of these properties on clinical diagnostic or therapeutic properties, however, requires the synthesis or purification of homogenous samples, which has proved to be difficult. While initial simulations indicated that size-selective separation could be achieved by flowing magnetic nanoparticles through a magnetic field, subsequent in vitro experiments were unable to reproduce the predicted results. Magnetic field-flow fractionation, however, was found to be an effective method for the separation of polydisperse suspensions of iron oxide nanoparticles with diameters greater than 20 nm. While similar methods have been used to separate magnetic nanoparticles before, no previous work has been done with magnetic nanoparticles between 20 and 200 nm. Both transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis were used to confirm the size of the MNPs. Further development of this work could lead to MNPs with the narrow size distributions necessary for their in vitro and in vivo optimization.

No MeSH data available.


Related in: MedlinePlus

(a) Digital image of magnetic separation prototype developed to replicate the conditions of the MATLAB simulation; (b) Digital image of Fraction 1 (left) and Fraction 2 (right) samples collected from the magnet distances of, from top to bottom, 11.5, 10, and 7.5 mm; (c–e) Size distributions of the obtained samples using distances of 7.5, 10, and 11.5 mm compared to that of the original MNP suspension.
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ijms-16-20001-f003: (a) Digital image of magnetic separation prototype developed to replicate the conditions of the MATLAB simulation; (b) Digital image of Fraction 1 (left) and Fraction 2 (right) samples collected from the magnet distances of, from top to bottom, 11.5, 10, and 7.5 mm; (c–e) Size distributions of the obtained samples using distances of 7.5, 10, and 11.5 mm compared to that of the original MNP suspension.

Mentions: A magnetic separation prototype, as seen in Figure 3a, was developed to reproduce the conditions of the simulation. As with the mathematical model, 1.6 mm I.D. tubing was used to replicate the 2D channel and 100 μm I.D. tubing was used to inject MNPs into the mobile phase at the wall opposite the magnets. Similar to conditions used in the simulation, the magnetic block was placed at distances of 7.5, 10, and 11.5 mm away from the center of the tubing, and a steady fluid flow velocity of 0.018 m/s was maintained using two syringe pumps.


Exploiting Size-Dependent Drag and Magnetic Forces for Size-Specific Separation of Magnetic Nanoparticles.

Rogers HB, Anani T, Choi YS, Beyers RJ, David AE - Int J Mol Sci (2015)

(a) Digital image of magnetic separation prototype developed to replicate the conditions of the MATLAB simulation; (b) Digital image of Fraction 1 (left) and Fraction 2 (right) samples collected from the magnet distances of, from top to bottom, 11.5, 10, and 7.5 mm; (c–e) Size distributions of the obtained samples using distances of 7.5, 10, and 11.5 mm compared to that of the original MNP suspension.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581337&req=5

ijms-16-20001-f003: (a) Digital image of magnetic separation prototype developed to replicate the conditions of the MATLAB simulation; (b) Digital image of Fraction 1 (left) and Fraction 2 (right) samples collected from the magnet distances of, from top to bottom, 11.5, 10, and 7.5 mm; (c–e) Size distributions of the obtained samples using distances of 7.5, 10, and 11.5 mm compared to that of the original MNP suspension.
Mentions: A magnetic separation prototype, as seen in Figure 3a, was developed to reproduce the conditions of the simulation. As with the mathematical model, 1.6 mm I.D. tubing was used to replicate the 2D channel and 100 μm I.D. tubing was used to inject MNPs into the mobile phase at the wall opposite the magnets. Similar to conditions used in the simulation, the magnetic block was placed at distances of 7.5, 10, and 11.5 mm away from the center of the tubing, and a steady fluid flow velocity of 0.018 m/s was maintained using two syringe pumps.

Bottom Line: Magnetic field-flow fractionation, however, was found to be an effective method for the separation of polydisperse suspensions of iron oxide nanoparticles with diameters greater than 20 nm.Both transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis were used to confirm the size of the MNPs.Further development of this work could lead to MNPs with the narrow size distributions necessary for their in vitro and in vivo optimization.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, USA. hunterrogers2014@u.northwestern.edu.

ABSTRACT
Realizing the full potential of magnetic nanoparticles (MNPs) in nanomedicine requires the optimization of their physical and chemical properties. Elucidation of the effects of these properties on clinical diagnostic or therapeutic properties, however, requires the synthesis or purification of homogenous samples, which has proved to be difficult. While initial simulations indicated that size-selective separation could be achieved by flowing magnetic nanoparticles through a magnetic field, subsequent in vitro experiments were unable to reproduce the predicted results. Magnetic field-flow fractionation, however, was found to be an effective method for the separation of polydisperse suspensions of iron oxide nanoparticles with diameters greater than 20 nm. While similar methods have been used to separate magnetic nanoparticles before, no previous work has been done with magnetic nanoparticles between 20 and 200 nm. Both transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis were used to confirm the size of the MNPs. Further development of this work could lead to MNPs with the narrow size distributions necessary for their in vitro and in vivo optimization.

No MeSH data available.


Related in: MedlinePlus