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Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.


Related in: MedlinePlus

Expression profiles of MaAQP genes in leaves of two cultivated banana varieties responding to cold, salt, and osmotic stresses. Log2-based FPKM values were used to create the heat-map with MeV software. The scale represents relative signal intensity of FPKM values.
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ijms-16-19728-f007: Expression profiles of MaAQP genes in leaves of two cultivated banana varieties responding to cold, salt, and osmotic stresses. Log2-based FPKM values were used to create the heat-map with MeV software. The scale represents relative signal intensity of FPKM values.

Mentions: Accumulated evidence has demonstrated that AQP genes play an important role in conferring plants resistance to abiotic stress, including drought, cold, and salt [3,22,23,24]. Thus, there is a need to investigate the transcriptional response of MaAQP genes to these abiotic stresses, which will benefit further functional characterization of AQP genes under stress conditions in banana. In the present study, a heat-map representing expression profiles of 40 MaAQP genes (except for MaPIP2-9, MaPIP1-9, MaPIP1-3, MaPIP1-2, MaTIP3-1, MaNIP1-2 and MaNIP2-5) was produced using transcriptomic data (Figure 7, Tables S10 and S11).


Genome-Wide Identification and Expression Analyses of Aquaporin Gene Family during Development and Abiotic Stress in Banana.

Hu W, Hou X, Huang C, Yan Y, Tie W, Ding Z, Wei Y, Liu J, Miao H, Lu Z, Li M, Xu B, Jin Z - Int J Mol Sci (2015)

Expression profiles of MaAQP genes in leaves of two cultivated banana varieties responding to cold, salt, and osmotic stresses. Log2-based FPKM values were used to create the heat-map with MeV software. The scale represents relative signal intensity of FPKM values.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4581322&req=5

ijms-16-19728-f007: Expression profiles of MaAQP genes in leaves of two cultivated banana varieties responding to cold, salt, and osmotic stresses. Log2-based FPKM values were used to create the heat-map with MeV software. The scale represents relative signal intensity of FPKM values.
Mentions: Accumulated evidence has demonstrated that AQP genes play an important role in conferring plants resistance to abiotic stress, including drought, cold, and salt [3,22,23,24]. Thus, there is a need to investigate the transcriptional response of MaAQP genes to these abiotic stresses, which will benefit further functional characterization of AQP genes under stress conditions in banana. In the present study, a heat-map representing expression profiles of 40 MaAQP genes (except for MaPIP2-9, MaPIP1-9, MaPIP1-3, MaPIP1-2, MaTIP3-1, MaNIP1-2 and MaNIP2-5) was produced using transcriptomic data (Figure 7, Tables S10 and S11).

Bottom Line: Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening.Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress.Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China. huwei2010916@126.com.

ABSTRACT
Aquaporins (AQPs) function to selectively control the flow of water and other small molecules through biological membranes, playing crucial roles in various biological processes. However, little information is available on the AQP gene family in bananas. In this study, we identified 47 banana AQP genes based on the banana genome sequence. Evolutionary analysis of AQPs from banana, Arabidopsis, poplar, and rice indicated that banana AQPs (MaAQPs) were clustered into four subfamilies. Conserved motif analysis showed that all banana AQPs contained the typical AQP-like or major intrinsic protein (MIP) domain. Gene structure analysis suggested the majority of MaAQPs had two to four introns with a highly specific number and length for each subfamily. Expression analysis of MaAQP genes during fruit development and postharvest ripening showed that some MaAQP genes exhibited high expression levels during these stages, indicating the involvement of MaAQP genes in banana fruit development and ripening. Additionally, some MaAQP genes showed strong induction after stress treatment and therefore, may represent potential candidates for improving banana resistance to abiotic stress. Taken together, this study identified some excellent tissue-specific, fruit development- and ripening-dependent, and abiotic stress-responsive candidate MaAQP genes, which could lay a solid foundation for genetic improvement of banana cultivars.

No MeSH data available.


Related in: MedlinePlus